Survey for Tick-Borne Zoonoses in the State of Espirito Santo, Southeastern Brazil

Spolidorio, Mariana Granziera; Labruna, Marcelo B.; Machado, Rosângela Zacarias; Moraes‐Filho, Jonas; Zago, Augusto M.; Donatele, Dirlei Molinari; Pinheiro, Sônia Regina; Silveira, Iara; Caliari, Késia M.; Nh, Yoshinari

doi:10.4269/ajtmh.2010.09-0595

Cited by 45 publications

(42 citation statements)

References 41 publications

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“…(4.4%) when compared to previous serological studies performed in dogs from endemic areas in the State of São Paulo that reported a seropositivity between 31.3 and 69.6% (LEMOS et al, 1996;HORTA et al, 2004HORTA et al, , 2007PINTER et al, 2008;MORAES-FILHO et al, 2008), from endemic areas of the State of Minas Gerais (13.7 and 81.3%) (LEMOS et al, 1994;VIANNA et al, 2008) and from endemic areas of the State of Espírito Santo (7.6%) (SPOLIDORIO et al, 2010). The seroprevalence found in the present study was also lower than that reported in dogs from non-endemic areas, 12.9% (LEMOS et al, 1996) and 20% (HORTA et al, 2007) in the State of São Paulo, and 11 to 23% in Northern Paraná (TAMEKUNI, 2009).…”

Section: Discussionmentioning

confidence: 80%

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

Fortes

Silveira

Moraes‐Filho

et al. 2010

Rev. Bras. Parasitol. Vet.

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Seroprevalence of Rickettsia bellii and AbstractBrazilian spotted fever (BSF) is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (IFA) against R. rickettsii and R. parkeri. All sera that reacted at least to one of Rickettsia species were tested against the six main Rickettsia species identified in Brazil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis. Sixteen samples (4.4%) reacted to at least one Rickettsia species. Among positive animals, two dogs (15.5%) showed suggestive titers for R. bellii exposure. One sample had a homologous reaction to R. felis, a confirmed human pathogen. Although Rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to R. bellii and R. felis in Southern Brazil.Keywords: Brazilian spotted fever, Rickettsia rickettsii, sentinels, indirect immunofluorescence assay. ResumoA febre maculosa brasileira (FMB) é uma zoonose veiculada por carrapatos e causada pela bactéria Rickettsia rickettsii, podendo os cães ser hospedeiros sentinelas para essa bactéria. O objetivo do estudo foi determinar a presença de anticorpos contra Rickettsia spp. em cães de São José dos Pinhais, estado do Paraná, Sul do Brasil. Entre fevereiro de 2006 e julho de 2007, amostras séricas de 364 cães foram coletadas e testadas na diluição de 1:64 por Reação de Imunofluorescência Indireta (RIFI) contra R. rickettsii e R. parkeri. Todos os soros reagentes para pelo menos uma espécie de Rickettsia foram testados contra as seis principais espécies de Rickettsia identificadas no Brasil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii e R. felis. Dezesseis amostras (4,4%) reagiram para pelo menos uma espécie de Rickettsia. Dos animais positivos, dois cães (15,5%) apresentaram títulos sugestivos de exposição a R. bellii. Uma amostra apresentou reação homóloga frente à R. felis, um agente patogênico confirmado para seres humanos. Muito embora os resultados demonstrem uma baixa prevalência de Rickettsia spp. em cães, sugerindo um baixo risco de infecção humana, este estudo relatou pela primeira vez a evidência de exposição a R. bellii e R. felis em cães no Sul do Brasil.Palavras-chave: Febre maculosa brasileira, Rickettsia rickettsii, sentinelas, reação de imunofluorescência indireta.

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Section: Discussionmentioning

confidence: 80%

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

Fortes

Silveira

Moraes‐Filho

et al. 2010

Rev. Bras. Parasitol. Vet.

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“…For these reasons, physicians must be aware to distinguish BYS from autoimmnune diseases. Additionally, we have found anemia, leucopenia, thrombocytopenia, transaminases increase and bilirrubinemia, mainly in BYS patients suspected of co-infections with other tick-borne diseases like babesiosis, ehrlichiosis, bartonellosis and rickettsiosis 7,35 .…”

Section: Discussionmentioning

confidence: 99%

Profile of patients with Baggio-Yoshinari Syndrome admitted at "Instituto de Infectologia Emilio Ribas"

Gouveia

Alves

Mantovani

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe aim of this study was to evaluate the epidemiological, clinical and laboratorial profile of patients with Baggio-Yoshinari Syndrome (BYS), who underwent internment at the Instituto de Infectologia Emilio Ribas in São Paulo, Brazil, during the period from July 1990 to July 2006. BYS is a new Brazilian tick-borne disease caused by Borrelia burgdorferi sensu lato microorganisms that resembles features of Lyme disease (LD), except for its epidemiological, clinical and laboratorial particularities. From 60 patients' records with positive serology to B. burgdorferi done by ELISA and Western-blotting methods, 19 cases were diagnosed as having BYS, according to criteria adopted at LIM-17 HCFMUSP, the Brazilian Reference Laboratory for the research of BYS. The other 41 remaining patients displayed miscellaneous infections or auto-immune processes. The beginning of symptoms in BYS group varied from one day to six years, from the onset of the disease. Four of 19 patients were included in acute disease stage, and 15 in latent. General unspecific symptoms were identified in almost all cases, with high frequencies of fever (78.9%) and lymphadenomegaly (36.8%). Six patients had skin lesions (31.5%); six arthralgia or arthritis (31.5%) and eight neurological symptoms (42%). Interestingly, two patients showed antibodies directed to B. burgdorferi exclusively in cerebrospinal fluid. Since BYS is a new emergent Brazilian zoonosis and its diagnosis is sometimes complex, all the new knowledge about BYS must be scattered to Brazilian Medical specialists, aiming to teach them how to diagnose this amazing tick-borne disease and to avoid its progression to chronic irreversible sequels.

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“…Although antibody detection may be of limited value for early serologic diagnosis (van ANDEL et al 1998), strong serological cross-reactions between all members of the A. phagocytophilum have been communicated (DUMLER et al, 1995). Recently, Spolidorio et al (2010) showed that 25.9% horses from the State of Espirito Santo, Brazil were reactive to at least one of the six Rickettsia species tested, and in six horses PCR Theileria were detected. It is important to note that no ticks of Ixodes ricinus, a common transmitter of EGA, have been described in Brazil.…”

Section: Discussionmentioning

confidence: 99%

Serologic evidence of equine granulocytic anaplasmosis in horses from central West Brazil

Salvagni

Dagnone

Gomes³

et al. 2010

Rev. Bras. Parasitol. Vet.

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Ehrlichiosis is a zoonotic disease caused by gram-negative and intracellular obligatory bacterial organisms. Equine Granulocytic Anaplasmosis - EGA (formerly Equine Granulocytic Ehrlichiosis, EGE) is a seasonal disease, normally self-limited in horses. There are few reports in Brazil about this ehrlichial agent, as well as its natural vectors. Nowadays, veterinarians are considering the suspicion of EGA in horses with suggestive symptoms of ehrlichiosis and which do not respond to piroplasmosis treatment. The aim of the present study was to identify horses exposed to the agent A. phagocytophilum by serological and molecular techniques. Twenty equine blood and serum samples from the central West region of Brazil were evaluated by microscopic examination of buffy coat smear, enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR). Additionally, the serodiagnosis of Theileria equi by IFA and ELISA were carried out, as well as molecular diagnosis by nPCR. Thirteen (65%) serum samples were positive for A. phagocytophilum by ELISA, but none of them were positive by buffy-coat smear examination or nPCR. Antibodies IgG anti-T. equi were detected in 18 (90%) and 17 (85%) horses by IFA and ELISA, respectively and the agent was detected in 9 (45%) animals by nPCR. Our data may be considered as important information to understanding the occurrence of EGA and equine piroplasmosis in central West Brazil.

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Survey for Tick-Borne Zoonoses in the State of Espirito Santo, Southeastern Brazil

Cited by 45 publications

References 41 publications

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

Profile of patients with Baggio-Yoshinari Syndrome admitted at "Instituto de Infectologia Emilio Ribas"

Serologic evidence of equine granulocytic anaplasmosis in horses from central West Brazil

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