Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

Abstract

Background: Chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. These bacterial populations cooperate to promote their own survival and the chronic nature of the infection. Few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. The use of 3 separate16S-based molecular amplifications followed by pyrosequencing, shotgun Sanger sequencing, and denaturing gradient gel electrophoresis were utilized to survey the major populations of bacteria that occur in the pathogenic biofilms of three types of chronic wound types: diabetic foot ulcers (D), venous leg ulcers (V), and pressure ulcers (P).

A systematic literature review of the human skin microbiome as biomarker for dermatological drug development

“…The role of the skin microbiome in UC was explored in 10 different studies, Table 1 37, 38, 39, 40, 41, 42, 43, 44, 45, 46. Current research into UC microbiome, comprises larger, longitudinal studies, compared to those in PV and HS.…”

Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair

“…Although chronic wounds contain both Gram-positive and Gram-negative species, the latter predominate, particularly in venous leg ulcers. 54 At the molecular level, an organism senses and responds to wound bacteria (ie, infection) via PRRs, initiating a highly orchestrated host immune response. LPS, the major component of all Trichrome-stained wound sections from mice subjected to LPS or PBS, collected 3 or 7 days after wounding.…”

Traumatic Wound Microbiome Workshop

“…Repeated debridement alone has a significant positive impact on healing, particularly when combined with the correction of systemic host factors (such as blood glucose management). Besides this universal approach to wounds, the clinic and diagnostic laboratories use molecular methods to determine the microbiota of each wound [33], which inhabits the wound in a structured community [34]. The therapeutic process that derives from the molecular diagnostics includes local antibiotics applied within a 3-day window of the debridement [35] to prevent reestablishment of the community.…”

“…Molecular methods change the diagnosis of many infections [33]. Wounds that provided no culturable bacteria often present numerous bacteria, including some expected to be culturable (such as Klebsiella).…”