Survey of duckweed diversity in Lake Chao and total fatty acid, triacylglycerol, profiles of representative strains

Tang, Jie; Li, Y.; Ma, Junjun; Ji, Cheng

doi:10.1111/plb.12345

Cited by 29 publications

(25 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed that strains representing L. aequinoctialis and S. polyrhiza were predominant, which was similar to duckweed diversity in Lake Chao (Tang et al . ). Subsequently, 20 duckweed strains were further investigated to assess best growth performance (Table ).…”

Section: Resultsmentioning

confidence: 99%

Large‐scale screening and characterisation of Lemna aequinoctialis and Spirodela polyrhiza strains for starch production

Zhu

et al. 2018

Plant Biol J

View full text Add to dashboard Cite

Duckweed is considered a promising feedstock for bioethanol production due to its high biomass and starch production. Selection of duckweed strains with high starch accumulation is essential for application of duckweeds to bioethanol production. Geographic differentiation had a large influence on genetic diversity of duckweeds. Biomass production, starch content and starch amount in geographically isolated strains of 20 Lemna aequinoctialis and Spirodela polyrhiza were calculated to evaluate their potential for bioethanol production. The influence of different collection time, culture medium and NaCl concentration on starch accumulation of the best strains were analysed. The results showed that biomass production, starch content and starch production of duckweeds demonstrated clonal dependency. The best strain was L. aequinoctialis 6000, with biomass production of 15.38 ± 1.47 g m , starch content of 28.68 ± 1.10% and starch production of 4.39 ± 0.25 g m . Furthermore, starch content of L. aequinoctialis 6000 was highest after 8 h of light, tap water was the best medium for starch induction, and NaCl did not induce starch accumulation. This study suggests duckweed biomass production and starch production demonstrate clonal dependency, indicating that extensive clonal comparisons will be required to identify the most suitable isolates for duckweed selective breeding for bioethanol.

show abstract

Section: Resultsmentioning

confidence: 99%

Large‐scale screening and characterisation of Lemna aequinoctialis and Spirodela polyrhiza strains for starch production

Zhu

et al. 2018

Plant Biol J

View full text Add to dashboard Cite

show abstract

“…In the present study, we found that the contribution of PUFA was 60% or higher for all analyzed Wolffia species, except for W. globosa 9498 (~54.5% PUFA). Tang et al ( 2015 ) investigated the fatty acid contents of the four species, Spirodela polyrhiza, Landoltia punctata, Lemna aequinoctialis , and W. globosa isolated from the lake Chao, China. Yan et al ( 2013 ) reported the most comprehensive survey of fatty acids in duckweeds by investigating 30 species (one clone per species) including eight species of Wolffia .…”

Section: Discussionmentioning

confidence: 99%

Nutritional Value of the Duckweed Species of the Genus Wolffia (Lemnaceae) as Human Food

et al. 2018

View full text Add to dashboard Cite

Species of the genus Wolffia are traditionally used as human food in some of the Asian countries. Therefore, all 11 species of this genus, identified by molecular barcoding, were investigated for ingredients relevant to human nutrition. The total protein content varied between 20 and 30% of the freeze-dry weight, the starch content between 10 and 20%, the fat content between 1 and 5%, and the fiber content was ~25%. The essential amino acid content was higher or close to the requirements of preschool-aged children according to standards of the World Health Organization. The fat content was low, but the fraction of polyunsaturated fatty acids was above 60% of total fat and the content of n-3 polyunsaturated fatty acids was higher than that of n-6 polyunsaturated fatty acids in most species. The content of macro- and microelements (minerals) not only depended on the cultivation conditions but also on the genetic background of the species. This holds true also for the content of tocopherols, several carotenoids and phytosterols in different species and even intraspecific, clonal differences were detected in Wolffia globosa and Wolffia arrhiza. Thus, the selection of suitable clones for further applications is important. Due to the very fast growth and the highest yield in most of the nutrients, Wolffia microscopica has a high potential for practical applications in human nutrition.

show abstract

“…Samples isolated from four locations were genotyped using two chloroplast markers and nuclear ribosomal DNA markers. The chloroplast DNA markers identified the samples as the greater duckweed, Spirodela polyrhiza , a dominant duckweed species in southern and middle China (Tang et al, 2015 ). The chloroplast ATP barcode showed some variability between samples, characteristic for the sequence variation between ecotypes of the same species (Supplement Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

Structural and Biochemical Properties of Duckweed Surface Cuticle

Borisjuk

Peterson

et al. 2018

Front. Chem.

View full text Add to dashboard Cite

The plant cuticle, which consists of cutin and waxes, forms a hydrophobic coating covering the aerial surfaces of all plants. It acts as an interface between plants and their surrounding environment whilst also protecting them against biotic and abiotic stresses. In this research, we have investigated the biodiversity and cuticle properties of aquatic plant duckweed, using samples isolated from four different locations around Hongze lake in Jiangsu province, China. The samples were genotyped using two chloroplast markers and nuclear ribosomal DNA markers, which revealed them as ecotypes of the larger duckweed, Spirodela polyrhiza. Duckweed cuticle properties were investigated by compositional analysis using Gas Chromatography coupled with Mass Spectroscopy (GC-MS) Flame Ionization Detector (GC-FID), and ultrastructural observation by cryo-Scanning Electron Microscopy (cryo-SEM). Cuticle compositional analysis indicated that fatty acids and primary alcohols, the two typical constituents found in many land plant cuticle, are the major duckweed wax components. A large portion of the duckweed wax fraction is composed of phytosterols, represented by campesterol, stigmasterol, sitosterol and their common precursor squalene. The cryo-SEM observation uncovered significant differences between the surface structures of the top air-facing and bottom water-facing sides of the plant fronds. The top side of the fronds, containing multiple stomata complexes, appeared to be represented by a rather flat waxy film sporadically covered with wax crystals. Underneath the waxy film was detected a barely distinguished nanoridge net, which became distinctly noticeable after chloroform treatment. On the bottom side of the fronds, the large epidermal cells were covered by the well-structured net, whose sections became narrower and sharper under cryo-SEM following chloroform treatment. These structural differences between the abaxial and adaxial sides of the fronds evidently relate to their distinct physiological roles in interacting with the contrasting environments of sunlight/air and nutrients/water. The unique structural and biochemical features of Spirodela frond surfaces with their rapid reproductive cycle and readily availability genome sequence, make duckweed an attractive monocot model for studying the fundamental processes related to plant protection against ultraviolet irradiation, pathogens and other environmental stresses.

show abstract

Survey of duckweed diversity in Lake Chao and total fatty acid, triacylglycerol, profiles of representative strains

Cited by 29 publications

References 45 publications

Large‐scale screening and characterisation of Lemna aequinoctialis and Spirodela polyrhiza strains for starch production

Large‐scale screening and characterisation of Lemna aequinoctialis and Spirodela polyrhiza strains for starch production

Nutritional Value of the Duckweed Species of the Genus Wolffia (Lemnaceae) as Human Food

Structural and Biochemical Properties of Duckweed Surface Cuticle

Contact Info

Product

Resources

About