Survey of Human Rotavirus Serotypes in Different Locales in Japan by Enzyme-Linked Immunosorbent Assay with Monoclonal Antibodies

Abstract

To investigate the relative frequency of individual human rotavirus serotypes prevailing in Japan, 562 stool specimens collected from patients with rotavirus gastroenteritis between November 1986 and March 1988 in seven districts were examined by an enzyme-linked immunosorbent assay (ELISA) with serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies. Serotype 1 was the predominant serotype in the winter of 1986-1987; however, both serotypes 1 and 2 were detected frequently in the winter of 1987-1988. The results showed the relative frequency of individual serotypes by locale and the yearly change in the prevalence of each serotype in the same area. The result of subgroup specificity of rotavirus obtained by using ELISA with subgroup I- and II-specific monoclonal antibodies confirmed the general finding that rotavirus strains having subgroup I specificity are serotype 2 and those having subgroup II specificity are either serotype 1, 3, or 4. Unusual strains having both subgroup I and II specificity or neither specificity and strains presumed to represent new serotypes were also found.

Molecular Characterization in the VP7, VP4 and NSP4 Genes of Human Rotavirus Serotype 4 (G4) Isolated in Japan and Kenya

“…At present, the genus Rotaviridae is classified into seven groups (groups A to G), based on group specific antigen. Group A rotavirus is the most prevalent (18,22). The genome of rotavirus comprises of 11 segments of double-stranded RNA, each of which encodes a distinct viral protein.…”

Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR

“…Since the serotype specificity has been determined mainly with regard to the outer capsid protein VP7, an enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies directed to VP7 can be used for serotype determination [5,6]. ELISA-serotyping has been widely and successfully employed to survey serotype distribution [7][8][9][10][11][12][13][14][15]. In addition, a polymerase chain reaction (PCR) to identify serotypes of rotavirus was also developed to assign rotavirus serotypes which could not been determined by ELISA-serotyping [16,17].…”

Elisas for Rotavirus Diagnosis, Typing, and Analysis of Antibody Response

“…Further development of Mabs that specifically neutralize other RVA serotypes, like G6, G8, G9, and G10, have been useful to increase the coverage of serotyping ELISAs to detect emerging or zoonotic RVA infections (Coulson et al, 1999;Keklar & Ayachit, 2000). Serotype-specific ELISAs have been used in numerous studies to determine the prevalence of different G serotypes in different parts of the world (Beards et al, 1989;Keklar & Ayachit, 2000;Urasawa et al, 1989). Serotype G1 is the most important serotype in humans throughout the world, accounting for more than 50% of all infections, and other serotypes of the Wa-like family, i. e. G3, G4, and G9, are also important, together with the single G2 serotype of the DS1-like family (Santos & Hoshino, 2005).…”