Survey of Shiga toxin-producing<i>Escherichia coli</i>O157:H7 in urban pigeons(<i>Columba livia</i>) in the city of Napoli, Italy

Santaniello, Antonio; Gargiulo, Antonio R.; Borrelli, Luca; Dipineto, Ludovico; Cuomo, Alessandra; Sensale, Mariangela; Fontanella, Marzia; Calabria, Mariarosaria; Musella, Vincenzo; Menna, Lucia Francesca; Fioretti, Alessandro

doi:10.4081/ijas.2007.313

Cited by 12 publications

(8 citation statements)

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“…In Italy, stx genes were detected in 10.8% of the stool enrichment cultures collected from feral pigeons [ 42 ]. In another study in Italy [ 9 ], four E. coli O157:H7 strains from pigeons were isolated; all strains carried eae and stx 2 genes, whereas only one strain carried the stx 1 gene. In Germany, 67% of the examined pigeon feces harbored stx genes [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…When infecting humans, they often cause bloody diarrhea, hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), and thrombotic thrombocytopenia purpura [ 4 , 5 , 8 ]. The most renowned example is E. coli O157:H7 which has been incriminated in human outbreaks since the 1980’s [ 9 - 12 ]. On the other hand, the recovery rate of non-O157:H7 STEC is the same or even exceeds that of O157:H7 [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…[ 16 , 17 ] lent support to the claim that wild birds are vectors and reservoirs for the maintenance and spread of STEC infections. The interactions between humans and wild birds are obvious; they reside in human habitats, migrate between waste collection areas, cattle, pig and poultry farms and deposit their droppings in soil and water and hence allowing the transmission of these zoonoses to man and animals [ 9 , 11 , 13 - 20 ]. The long survival time of STEC in soil (for up to 7 months) may give the opportunity for such transmission [ 10 , 21 - 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species

2017

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Aim:Wild birds are considered silent vectors of some zoonotic water and food borne pathogens of public health significance. Owing to the importance of Shiga toxin producing Escherichia coli (STEC) as the most pathogenic among the emerging diarrheagenic E. coli groups that can infect man; the present study was designed to detect the occurrence of STEC among wild birds in Egypt.Materials and Methods:A total of 177 intestinal content swab samples originating from five wild bird species were investigated for the presence of E. coli and STEC by standard culture methods. Suspect STEC isolates were further characterized by serotyping, random amplified polymorphic DNA polymerase chain reaction (RAPD PCR), antimicrobial resistance pattern and PCR detection of stx1, stx2, and eae genes.Results:A total of 30 suspect STEC isolates from 30 positive birds’ samples were detected and identified on STEC CHROMagar (semi-captive pigeons, 15; house crows, 8; cattle egrets, 3; moorhens, 2; and house teals, 2). 25 isolates were grouped into 13 serogroups (O:20, O:25, O:26, O:27, O:63, O:78, O:111, O:114, O:125, O:128, O:142, O:153, and O:158), while five were rough strains. The distribution of STEC virulence genes among wild birds was as follows: 16 birds carried stx1 gene only (nine pigeons [28.1%], six crows [7.1%], and one cattle egret [5.6%]). Stx1 and stx2 genes together were detected in four birds (one cattle egret [5.6%], two moorhens [6.1%], and one house teal, [10%]). Only one pigeon (3.1%) possessed the three alleles. Disk diffusion test results showed that cefixime was the most effective against STEC serotypes with (93.3%) sensitivity, followed by gentamycin (56.7%), and amoxicillin (50%). On the other hand, all the recovered STEC isolates were resistant to cefotaxime, doxycycline, cephalothin, and sulfisoxazole. RAPD fingerprinting using primers OPA-2 and OPA-9 showed that STEC isolates were heterogeneous; they yielded 30 and 27 different clusters, respectively.Conclusions:Wild birds carry STEC and may add to the contamination of the surrounding environment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species

2017

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“…Some studies show that enterohemorrhagic serotype O157:H7 of E . coli can be present in feral pigeons 15 , 28 . Moreover, in Peru there have been reported outbreaks of human enterohaemorrhagic colibacilosis from unknown sources of infection 13 , although prevalence of STEC in children is up to 9% while EPEC is higher 21 .…”

Section: Introductionmentioning

confidence: 99%

ISOLATION AND MOLECULAR IDENTIFICATION OF POTENTIALLY PATHOGENIC Escherichia coli AND Campylobacter jejuni IN FERAL PIGEONS FROM AN URBAN AREA IN THE CITY OF LIMA, PERU

Caballero

Rivera

Jara

et al. 2015

Rev. Inst. Med. trop. S. Paulo

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SUMMARYFeral pigeons (Columbia livia) live in close contact with humans and other animals. They can transmit potentially pathogenic and zoonotic agents. The objective of this study was to isolate and detect strains of diarrheagenic Escherichia coli and Campylobacter jejuni of urban feral pigeons from an area of Lima, Peru. Fresh dropping samples from urban parks were collected for microbiological isolation of E. coli strains in selective agar, and Campylobacter by filtration method. Molecular identification of diarrheagenic pathotypes of E.coli and Campylobacter jejuni was performed by PCR. Twenty-two parks were sampled and 16 colonies of Campylobacter spp. were isolated. The 100% of isolates were identified as Campylobacter jejuni. Furthermore, 102 colonies of E. coliwere isolated and the 5.88% resulted as Enteropathogenic (EPEC) type and 0.98% as Shiga toxin-producing E. coli (STEC). The urban feral pigeons of Lima in Peru can act as a reservoir or carriers of zoonotic potentially pathogenic enteric agents.

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“…coli is found naturally as intestinal microflora of many species including pigeons. Pigeons are extremely effective transmitters of E. coli to humans because their feces is a source of E. coli O157:H7 for birds, mammals and humans (Santaniello et al, 2007). E. coli is a Gram-negative, rod-shaped, flagellated, motile, oxidase negative, facultative anaerobic bacteria under the family Enterobacteriaceae (Buxton and Fraser, 1977).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

et al. 2014

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A B S T R A C TTo determine prevalence of antimicrobial resistant Escherichia coli in pigeon a total of 112 samples such as cloacal swabs (n=36), foot pads (n=36) and feces (n=40) were collected from pigeon aseptically. Samples were enriched in nutrient broth and then streaked onto eosine methylene blue agar, Salmonella-Shigella agar, MacConkey agar and blood agar. The cultural and biochemical characteristics of bacterial isolates of pigeon were indicative of E. coli. The prevalence of E. coli in cloacal swabs, foot pads and feces samples were 86.11%, 44.44% and 77.50%, respectively. The overall prevalence of E. coli in pigeon was 69.64% (78 of 122 samples were found positive for E. coli). The antibiotic sensitivity pattern showed that E. coli isolates were sensitive to erythromycin, ciprofloxacin, kanamycin, nalidixic acid and resistant to amoxicillin, tetracycline and sulphamethaxazole. It may be concluded that pigeons from Mymensingh locality in Bangladesh carry multidrug resistant E. coli. Data of this study will be helpful in creating public health awareness among people and help controlling transmission of drug resistant E. coli by undertaking effective sanitary and hygienic measures.

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Survey of Shiga toxin-producingEscherichia coliO157:H7 in urban pigeons(Columba livia) in the city of Napoli, Italy

Cited by 12 publications

References 3 publications

Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species

Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species

ISOLATION AND MOLECULAR IDENTIFICATION OF POTENTIALLY PATHOGENIC Escherichia coli AND Campylobacter jejuni IN FERAL PIGEONS FROM AN URBAN AREA IN THE CITY OF LIMA, PERU

Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

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