Survey of the susceptibility of urinary isolates to antibacterial agents in 2003

Ishikawa, Kiyohito; Hayakawa, Satoshi; Miyakawa, Shinzaburo; Kusaka, Mamoru; Shiroki, Ryoichi; Hoshinaga, Kiyotaka

doi:10.1007/s10156-004-0356-9

Cited by 16 publications

(21 citation statements)

References 11 publications

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“…Humans (Ishikawa et al, 2005), food animals (Asai et al, 2005) and wildlife (Gilliver et al, 1999) may act as reservoirs of drug-resistant bacteria through the food chain. The widespread occurrence of antimicrobial resistance in bacteria in wildlife populations may be caused by environmental pollution of antimicrobials or resistant bacteria through human activities such as antimicrobial use in medicine and agriculture.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

et al. 2014

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A B S T R A C TTo determine prevalence of antimicrobial resistant Escherichia coli in pigeon a total of 112 samples such as cloacal swabs (n=36), foot pads (n=36) and feces (n=40) were collected from pigeon aseptically. Samples were enriched in nutrient broth and then streaked onto eosine methylene blue agar, Salmonella-Shigella agar, MacConkey agar and blood agar. The cultural and biochemical characteristics of bacterial isolates of pigeon were indicative of E. coli. The prevalence of E. coli in cloacal swabs, foot pads and feces samples were 86.11%, 44.44% and 77.50%, respectively. The overall prevalence of E. coli in pigeon was 69.64% (78 of 122 samples were found positive for E. coli). The antibiotic sensitivity pattern showed that E. coli isolates were sensitive to erythromycin, ciprofloxacin, kanamycin, nalidixic acid and resistant to amoxicillin, tetracycline and sulphamethaxazole. It may be concluded that pigeons from Mymensingh locality in Bangladesh carry multidrug resistant E. coli. Data of this study will be helpful in creating public health awareness among people and help controlling transmission of drug resistant E. coli by undertaking effective sanitary and hygienic measures.

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Section: Introductionmentioning

confidence: 99%

Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

et al. 2014

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“…If left untreated, these infections can lead to more serious conditions including acute pyelonephritis, bacteremia, and renal scarring. Furthermore, increasing rates of antimicrobial resistance among uropathogens will likely complicate future treatment of these infections (13,21). Consequently, there is an urgent public health need to develop an efficacious vaccine to prevent UTI.…”

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confidence: 99%

Uropathogenic Escherichia coli Outer Membrane Antigens Expressed during Urinary Tract Infection

2007

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Uncomplicated urinary tract infection (UTI) caused by uropathogenic Escherichia coli (UPEC) represents a prevalent and potentially severe infectious disease. In this study, we describe the application of an immunoproteomics approach to vaccine development that has been used successfully to identify vaccine targets in other pathogenic bacteria. Outer membranes were isolated from pyelonephritis strain E. coli CFT073 cultured under conditions that mimic the urinary tract environment, including iron limitation, osmotic stress, human urine, and exposure to uroepithelial cells. To identify antigens that elicit a humoral response during experimental UTI, outer membrane proteins were separated by two-dimensional gel electrophoresis and probed using pooled antisera from 20 CBA/J mice chronically infected with E. coli CFT073. In total, 23 outer membrane antigens, including a novel iron compound receptor, reacted with the antisera and were identified by mass spectrometry. These antigens also included proteins with known roles in UPEC pathogenesis, such as ChuA, IroN, IreA, Iha, IutA, and FliC. These data demonstrate that an antibody response is directed against these virulenceassociated factors during UTI. We also show that the genes encoding ChuA, IroN, hypothetical protein c2482, and IutA are significantly more prevalent (P < 0.01) among UPEC strains than among fecal-commensal E. coli isolates. Thus, we suggest that the conserved outer membrane antigens identified in this study could be rational candidates for a UTI vaccine designed to elicit protective immunity against UPEC infection.

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“…Most of the resistant E. coli isolates from food animals were found to be sulfadimethoxine-resistant, oxytetracycline-resistant, and dihydrostreptomycin-resistant (Kijima-Tanaka et al, 2003). Cephem-resistant bacteria were rarely detected in Japanese livestock (Asai et al, 2005), but they have been found in companion animals (Pedersen et al, 2007) and humans (Ishikawa et al, 2005). In this study, all four resistant E. coli isolates from wild macaques were cephalothin-resistant.…”

Section: Discussionmentioning

confidence: 48%

“…Humans (Ishikawa et al, 2005), food animals (Asai et al, 2005), and wildlife (Gilliver et al, 1999) may act as reservoirs of drug-resistant bacteria through the food chain. Antimicrobial resistance was found to be widespread in enterobacteria from wild rodents in England (Gilliver et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Genetic Characteristics and Antimicrobial Resistance of Escherichia Coli From Japanese Macaques (Macaca Fuscata) in Rural Japan

Ogawa

Yamaguchi

Suzuki

et al. 2011

Journal of Wildlife Diseases

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ABSTRACT:Escherichia coli was isolated from wild and captive Japanese macaques (Macaca fuscata) to investigate the risk of zoonotic infections and the prevalence of antimicrobial-resistant Escherichia coli in the wild macaque population in Shimokita Peninsula, a rural area of Japan. We collected 265 fresh fecal samples from wild macaques and 20 samples from captive macaques in 2005 and 2006 for E. coli isolation. The predominant isolates were characterized by serotyping, virulence gene profiling, plasmid profiling, pulsed-field gel electrophoresis (PFGE), and microbial sensitivity tests. In total, 248 E. coli strains were isolated from 159 fecal samples from wild macaques, and 42 E. coli were isolated from 17 samples from captive macaques. None of the virulence genes eae, stx, elt, and est were detected in any of the isolates. The relatedness between wild-and captive-derived isolates was low by serotyping, PFGE, and plasmid profiling. Serotypes O8:H6, O8:H34, O8:H42, O8:HUT, O103:H27, O103:HNM, and OUT:H27 were found in wild macaque feces; serotypes O157:H42 and O119:H21 were recovered from captive macaques. Oand H-serotypes of the 26 isolates were not typed by commercial typing antisera and were named OUT and HUT, respectively. Twenty-eight isolates had no flagellar antigen, and their H-serotypes were named HNM. Similarity of PFGE patterns between wild-derived isolates and captivederived isolates was ,70%. No plasmid profile was shared between wild-derived and captivederived isolates. The prevalence of antimicrobial-resistant E. coli was 6.5% (n562) in wild macaques, and these isolates were resistant to cephalothin. We conclude that wild Japanese macaques in Shimokita Peninsula were unlikely to act as a reservoir of pathogenic E. coli for humans and that antimicrobial-resistant E. coli in wild macaques may be derived from humans.

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Survey of the susceptibility of urinary isolates to antibacterial agents in 2003

Cited by 16 publications

References 11 publications

Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

Uropathogenic Escherichia coli Outer Membrane Antigens Expressed during Urinary Tract Infection

Genetic Characteristics and Antimicrobial Resistance of Escherichia Coli From Japanese Macaques (Macaca Fuscata) in Rural Japan

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