Survey on Antibiotic Residues in Egg Samples in Italy

Saluti, Giorgio; Colagrande, Maria Novella; Castellani, Federica; Ricci, Matteo; Diletti, Gianfranco; Scortichini, Giampiero

doi:10.3390/separations8090148

Cited by 6 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on these ndings, the developed method was applied to real samples, which were obtained from supermarkets. Sulfamethoxazole (SMX) was detected in all analyzed samples in the range of 9.56-46.3 µg kg − 1 with the corresponding %RSD values ranging from 8.8 to 15.2% indicating non-compliance as sulfonamides are prohibited in laying hens (Saluti et al 2021). Since there is a small peak present at the same retention time as #11 in the blank egg sample, additional con rmation of the presence of SMX (by MS) would be recommended for future work.…”

Section: Discussionmentioning

confidence: 99%

“…Many countries and organizations have established maximum residue limits (MRLs) for sulfonamides in foods of animal origin; for example, the European Union (EU) has established a maximum residue limit (MRL) of 100 µg kg -1 for total sulfonamides in foods of animal origin (EC regulation 37/2010).However, for eggs, where no residue limits have been set for sulfonamides there is "zero tolerance" limit, meaning that no residues should be permitted (Forti and Scortichini 2009;Frenich et al 2010;Saluti et al 2021).Therefore, monitoring of these compounds at a trace level is very important to comply with the above requirement, especially if the animal product is intended for human consumption.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dispersive Liquid - Liquid Microextraction for the Determination of Sulfonamide Residues i n Egg Samples

Bekele

Dube

Nindi

2022

Preprint

View full text Add to dashboard Cite

A dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-diode array detection was developed for the extraction and determination of 15 sulfonamide residues in the egg matrix. The effects of various parameters such as the type, volume, and composition of extraction solvent for sample treatment procedure, the type and volume of disperser solvent, centrifugation time, salting-out effect and solution pH were studied, and optimum conditions were established. Linearity was found in the range of 5.4- 1 000 µg kg-1 with regression coefficients ranging from 0.9918–0.9987. The limit of detection (LOD) and limit of quantification (LOQ) values of the proposed method were in the range of 4.3–8.0 µg kg-1 and 12.9–24.0 µg kg-1, respectively. Satisfactory intra-day and inter-day precision results in the range of 6.3–17.5% and 4.8–16.8%, respectively, were achieved. The accuracy (percentage recovery) and precision (relative standard deviation (RSD)) of the method were also evaluated. The accuracy of the method was acceptable with percentage recovery in the range of 73–108% and %RSD values in the range of 1.1–16.5%. The proposed method was applied in chicken egg samples obtained from supermarkets, and findings confirmed that the method is feasible to be used for extraction and determination sulfonamide residues in egg and related complex biological matrices.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dispersive Liquid - Liquid Microextraction for the Determination of Sulfonamide Residues i n Egg Samples

Bekele

Dube

Nindi

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Based on these findings, the developed method was applied to real samples, which were obtained from supermarkets. Sulfamethoxazole (SMX) was detected in all analyzed samples in the range of 9.56 -46.3 µg kg-1 with the corresponding %RSD values ranging from 8.8 to 15.2% indicating non-compliance as sulfonamides are prohibited in laying hens [3]. Since there is a small peak present at the same retention time as #11 in the blank egg sample, additional confirmation of the presence of SMX (by MS) would be recommended for future work.…”

Section: Discussionmentioning

confidence: 99%

“…Many countries and organizations have established maximum residue limits (MRLs) for sulfonamides in foods of animal origin; for example, the European Union (EU) has established a maximum residue limit (MRL) of 100 µg kg-1 for total sulfonamides in foods of animal origin (EC regulation 37/2010). However, for eggs, where no residue limits have been set for sulfonamides there is "zero tolerance" limit, meaning that no residues should be permitted [1][2][3]. Therefore, monitoring of these compounds at a trace level is very important to comply with the above requirement, especially if the animal product is intended for human consumption.…”

Section: Introductionmentioning

confidence: 99%

Dispersive Liquid - Liquid Microextraction for the Determination of Sulfonamide Residues in Egg Samples

2023

BSCR

View full text Add to dashboard Cite

A dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-diode array detection was developed for the extraction and determination of 15 sulfonamide residues in the egg matrix. The effects of various parameters such as the type, volume, and composition of extraction solvent for sample treatment procedure, the type and volume of disperser solvent, centrifugation time, salting-out effect and solution pH were studied, and optimum conditions were established. Linearity was found in the range of 5.4 – 1 000 μg kg-1 with regression coefficients ranging from 0.9918 - 0.9987. The limit of detection (LOD) and limit of quantification (LOQ) values of the proposed method were in the range of 4.3 - 8.0 μg kg-1 and 12.9 - 24.0 μg kg-1, respectively. Satisfactory intra-day and inter-day precision results in the range of 6.3 - 17.5% and 4.8 - 16.8%, respectively, were achieved. The accuracy of the method was acceptable with percentage recovery in the range of 73 - 108% and %RSD values in the range of 1.1 - 16.5%. The proposed method was applied in chicken egg samples obtained from supermarkets, and findings confirmed that the method is feasible to be used for extraction and determination sulfonamide residues in egg and related complex biological matrices.

show abstract

“…However, HPLC methods, although more laborious, are more accurate, reproducible and precise than the aforementioned, being appropriate for pharmacokinetic studies. Therefore, liquid chromatography coupled with mass spectrometry (HPLC/MS) techniques have been widely used in previous studies to quantify TV in feed, milk, honey, tissues and eggs [ 8 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Quantification and Determination of Stability of Tylvalosin in Pig Plasma by Ultra-High Liquid Chromatography with Ultraviolet Detection

Hernandis

Escudero

Galecio

et al. 2022

Animals

View full text Add to dashboard Cite

Tylvalosin (TV) is a macrolide antibiotic that is used for treating respiratory and enteric bacterial infections in swine and in poultry. In the coming years, the use of this drug will probably be widely studied in different species, but before its use in each veterinary species, macrolide analytical determination in various biological fluids is a pre-requisite step for the rational dose calculation of TV based on specific pharmacokinetic information. Its quantification is essential for detecting and avoiding the appearance of residues in animal products intended for human consumption. Therefore, a robust chromatographic method coupled with an ultraviolet detector was fully validated for the quantification of TV in pig plasma. A mixture (78:22) of (A) 0.3% formic acid in water and (B) acetonitrile was used as the mobile phase. TV and enrofloxacin (internal standard) were eluted at 14.1 and 5.9 min, respectively. Calibration curves ranged from 0.1 to 5 μg/mL. The accuracy and precision parameters for the quality controls were always <13.0%. Recovery ranged from 89.66 to 96.92%. The detection and quantification limits were found to be 0.05 μg/mL and 0.1 μg/mL, respectively. This method could be applied to develop pharmacokinetic studies.

show abstract

Survey on Antibiotic Residues in Egg Samples in Italy

Cited by 6 publications

References 24 publications

Dispersive Liquid - Liquid Microextraction for the Determination of Sulfonamide Residues i n Egg Samples

Dispersive Liquid - Liquid Microextraction for the Determination of Sulfonamide Residues i n Egg Samples

Dispersive Liquid - Liquid Microextraction for the Determination of Sulfonamide Residues in Egg Samples

Quantification and Determination of Stability of Tylvalosin in Pig Plasma by Ultra-High Liquid Chromatography with Ultraviolet Detection

Contact Info

Product

Resources

About