Survival advantage depends on cecal volume rather than cecal length in a mouse model of cecal ligation and puncture

Song, Tian-Zhang; Yin, Hongling; Chen, Jintao; Huang, Lilin; José, Juán; He, Tailong; Huang, Huaiqiu; Hu, Xuchu

doi:10.1016/j.jss.2016.03.019

Cited by 9 publications

(11 citation statements)

References 18 publications

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“…Despite these limitations, this work demonstrates that the microbiome independently alters mortality and immune response following sepsis. Controlling for the microbiome [similar to controlling for age, gender, genetics, and surgical details such as needle gauge and length of cecum ligated (44,45)] should be considered when performing preclinical studies on sepsis in order to maximize the translation of findings. Further work needs to be done to understand the mechanisms underlying the role of the microbiome in mortality that can potentially be manipulated for therapeutic gain in patients with sepsis.…”

Section: Discussionmentioning

confidence: 99%

The gut microbiome alters immunophenotype and survival from sepsis

Fay¹,

Klingensmith²,

Chen³

et al. 2019

FASEB j.

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The microbiome is increasingly implicated in immune regulation and mortality from sepsis. Mice with identical genetic backgrounds but distinct microbiomes were obtained from different vendors and analyzed following cecal ligation and puncture (CLP), β diversity of the microbiome measured from feces demonstrated significant differences between The Jackson Laboratory (Jax; Bar Harbor, ME, USA) and Charles River Laboratories (CR; Wilmington, MA, USA) C57/B6 mice. Jax mice had 7‐d mortality of 90% following CLP, whereas CR mice had a mortality of 53%. Differences in vendor were associated with altered immunophenotype with increased splenic IFN‐γ+CD4+ T cells, effector memory CD4+ T cells, and central memory CD4+ T cells and increased Peyer's patch effector memory CD4+ T cells in septic CR mice. To determine whether differences in the microbiome were responsible for these differences, Jax and CR mice were cohoused for 3 wk, after which they assumed a similar microbiota composition. Cohoused mice had improved survival following CLP compared to Jax mice and had similar survival regardless of their vendor of origin. All differences in immunophenotype between septic Jax and CR mice disappeared following cohousing. These findings suggest that the microbiome plays a crucial role in survival and the host immune response from sepsis and represents a potential target for therapeutic intervention.—Fay, K. T., Klingensmith, N. J., Chen, C.‐W., Zhang, W., Sun, Y., Morrow, K. N., Liang, Z., Burd, E. M., Ford, M. L., Coopersmith, C. M. The gut microbiome alters immunophenotype and survival from sepsis. FASEB J. 33, 11258–11269 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 99%

The gut microbiome alters immunophenotype and survival from sepsis

Fay¹,

Klingensmith²,

Chen³

et al. 2019

FASEB j.

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“…Our results were in agreement with other studies, which showed that MPO activity as neutrophil accumulations was elevated by sepsis, while pretreatment with selenium, n-acetylcysteine, and simvastatin decreased MPO activity by scavenging free radical generation, which suppressed the severity of sepsis. 10,29,30 One of the consequences of sepsis is an event called lipid peroxidation, which is considered as the main oxidative stress parameter and which was increased by the impact of free radicals and MPO increment. 10 Lipid peroxidation can cause cell and mitochondria damage, which initiate protein degeneration, cell lysis, and necrosis.…”

Section: Discussionmentioning

confidence: 99%

“…9 All of them try to simulate pathophysiological changes, which are very similar to septic shock. 10 Among them, CLP is the most accepted method to imitate human sepsis; it is considered a gold standard model. For the first time, Chaudhry in 1970 developed CLP among others.…”

Section: Introductionmentioning

confidence: 99%

Considering the Effect of <i>Rosa damascena</i> Mill. Essential Oil on Oxidative Stress and <i>COX-2</i> Gene Expression in the Liver of Septic Rats

Dadkhah

Fatemi

Malayeri

et al. 2019

tjps

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Sepsis, tüm dünyada yüksek ölüm oranına sahip klinik bir hastalıktır. Oksidatif stres sepsiste ortaya çıkan önemli bir olgu olarak kabul edilir. Rosa damascena Mill., farmakolojik aktiviteleri yüksek olan eski bir bitkidir. Gereç ve Yöntemler:Standart bir model olarak çekal ligasyon ve ponksiyon (CLP), sıçanlarda sepsis oluşturmak için kullanılmıştır. Erkek yetişkin sıçanlar rastgele 5 gruba ayrılmıştır. R. damascena uçucu yağları (50 ve 100 mg/kg) 14 gün boyunca oral yolla verilmiş ve 15. günde CLP uygulanmıştır. Yirmi dört saat sonra, plazma plazma prostaglandin E 2 (PGE 2 ) ve COX-2 ekspresyonu ile birlikte oksidatif stres (MPO, MDA, GSH, GST ve FRAP) ve biyokimyasal parametreleri [alkalen fosfataz, aspartate transaminase (AST), alanin transaminaz (ALT) ve bilirubin] ölçmek için kan örnekleri ve karaciğer dokuları çıkarılmıştır. ÖZObjectives: Sepsis is a clinical illness with a high rate of mortality all over the world. Oxidative stress is considered the main phenomenon that occurs in sepsis. Rosa damascena Mill. is an ancient herbal plant with high pharmacological activities. Materials and Methods: Cecal ligation and puncture (CLP) as a standard model was used to induce sepsis in rats. Male adult rats were randomly divided into 5 groups. Different doses of R. damascena essential oil (50 and 100 mg/kg.bw) were gavaged orally for 14 days and on day 15 CLP was performed. After 24 h, blood samples and liver tissues were removed in order to measure oxidative stress [myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH), glutathione-S-transferase, and ferric reducing ability of plasma (FRAP)] and biochemical parameters [alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT), and bilirubin] together with plasma prostaglandin E 2 (PGE 2 ) and COX-2 expression. Results: The essential oil was capable of modulating all of the oxidative stress, antioxidant, and anti-inflammatory parameters induced by CLP as characterized by elevations in MPO and MDA levels as well as increases in AST and ALT concentrations concomitant with PGE 2 and COX-2 increments. The antioxidant defense system such as GSH and FRAP was also increased in the essential oil treated groups. Conclusion: Our results showed that the essential oil has antioxidative and hepatoprotective activities through reducing the oxidative injury in sepsis caused by CLP.

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“…aegypti protects mice against oxidative stress in a CLP model, which is considered the gold standard sepsis model [ 31 ] and most closely represents the clinical scenario with progression of sepsis [ 32 ]. In this model, the severity of sepsis can be adjusted by increasing the needle puncture size, number of punctures, or length of cecal ligation [ 31 , 33 , 34 ]. Thus, we performed a moderate CLP (50%, two punctures with a 22G needle) so that the animals died in the 6 days after CLP [ 21 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Salivary Gland Extract from Aedes aegypti Improves Survival in Murine Polymicrobial Sepsis through Oxidative Mechanisms

Gomes

Navegantes-Lima

Monteiro

et al. 2018

Cells

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Sepsis is a systemic disease with life-threatening potential and is characterized by a dysregulated immune response from the host to an infection. The organic dysfunction in sepsis is associated with the production of inflammatory cascades and oxidative stress. Previous studies showed that Aedes aegypti saliva has anti-inflammatory, immunomodulatory, and antioxidant properties. Considering inflammation and the role of oxidative stress in sepsis, we investigated the effect of pretreatment with salivary gland extract (SGE) from Ae. aegypti in the induction of inflammatory and oxidative processes in a murine cecum ligation and puncture (CLP) model. Here, we evaluated animal survival for 16 days, as well as bacterial load, leukocyte migration, and oxidative parameters. We found that the SGE pretreatment improved the survival of septic mice, reduced bacterial load and neutrophil influx, and increased nitric oxide (NO) production in the peritoneal cavity. With regard to oxidative status, SGE increased antioxidant defenses as measured by Trolox equivalent antioxidant capacity (TEAC) and glutathione (GSH), while reducing levels of the oxidative stress marker malondialdehyde (MDA). Altogether, these data suggest that SGE plays a protective role in septic animals, contributing to oxidative and inflammatory balance during sepsis. Therefore, Ae. aegypti SGE is a potential source for new therapeutic molecule(s) in polymicrobial sepsis, and this effect seems to be mediated by the control of inflammation and oxidative damage.

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Survival advantage depends on cecal volume rather than cecal length in a mouse model of cecal ligation and puncture

Cited by 9 publications

References 18 publications

The gut microbiome alters immunophenotype and survival from sepsis

The gut microbiome alters immunophenotype and survival from sepsis

Considering the Effect of <i>Rosa damascena</i> Mill. Essential Oil on Oxidative Stress and <i>COX-2</i> Gene Expression in the Liver of Septic Rats

Salivary Gland Extract from Aedes aegypti Improves Survival in Murine Polymicrobial Sepsis through Oxidative Mechanisms

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