Survival in the soil of the ectomycorrhizal fungus Laccaria bicolor and the effects of a mycorrhiza helper Pseudomonas fluorescens

Brulé, C.; Frey‐Klett, Pascale; Pierrat, Jean-Claude; Courrier, Sébastien; Gérard, Frédéric; Lemoine, Michel; Rousselet, J.L; Sommer, Georges; Garbaye, Jean

doi:10.1016/s0038-0717(01)00090-6

Cited by 74 publications

(53 citation statements)

References 25 publications

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“…The reasons are decreased photosynthetic activity of needles under lower illumination and utilization of almost all available assimilates for plant regeneration. ECM formation can be stimulated by soil bacteria -plant growth promoting rhizobacteria (höf-lich et al 2001), especially in adverse conditions for the development of fungi (Brulé et al 2001). The rooting substrate amended with bacteria in our experiment (product BactoFil B) did not affect the ECM development; the result coincides with findings of Shishido et al (1996).…”

Section: Discussionmentioning

confidence: 99%

Testing of microbial additives in the rooting of Norway spruce (Picea abies [L.] Karst.) stem cuttings

Repáč¹,

Vencurik²,

Balanda³

2011

J. For. Sci.

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Laboratory-produced alginate-bead inoculum of ectomycorrhizal (ECM) fungi Cortinarius sp. and Gomphidius glutinosus, fungal commercial products ECtovit® and Trichomil®, bacterial commercial product BactoFil B®, and commercial rooting stimulator Vetozen® were applied to a peat-perlite (1:2, v:v) rooting substrate of Norway spruce stem cuttings collected from 4-year-old nursery-grown seedlings immediately before the insertion of cuttings into the substrate. The application of beads free of fungi and the substrate without an additive were the other treatments. The cuttings were rooted in vessels (60 cuttings per vessel, 180 per treatment) placed in a glasshouse and arranged in a randomized complete block design. The cuttings were estimated for one growing season (approximately for 26 weeks) after their insertion into the rooting substrate. Rooting % of the cuttings ranged from 45 (mycelium-free beads) to 75 (control) according to treatments, 64 on average. No significant differences among treatments were found in % of ECM morphotypes, total ECM colonization of roots (%), and growth parameters of shoots and roots of the cuttings. The applied microbial additives were not sufficiently efficient to form treatment-related ectomycorrhizas that were formed by naturally occurring ECM fungi. Inoculation by the ECM fungus Cortinarius sp. and application of Trichomil had a partial stimulative effect on the shoot growth of cuttings. Shoot and root growth parameters were not significantly correlated with total ECM colonization, except for a negative dependence of the root number in Trichomil treatment. A higher concentration of K but lower concentrations of Ca and Mg in Ectovit treatment than in the other treatments were detected.

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Section: Discussionmentioning

confidence: 99%

Testing of microbial additives in the rooting of Norway spruce (Picea abies [L.] Karst.) stem cuttings

Repáč¹,

Vencurik²,

Balanda³

2011

J. For. Sci.

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“…If MHB inoculation leads to increased mycelial biomass in the soil, the occurrence of rootfungus encounters should increase too, resulting in faster mycorrhization (Brulé et al 2001). In line with this hypothesis, a significant correlation has been shown to exist between improved mycelial extension and promoted mycorrhiza establishment (Garbaye and Bowen 1989;Garbaye and Duponnois 1992;Gryndler and Vosatka 1996;Founoune et al 2002;Schrey et al 2005;Riedlinger et al 2006).…”

Section: Promoted Mycelial Growthmentioning

confidence: 82%

“…In a long-term survey, Brulé et al (2001) trapped fungal mycelium with Douglas fir seedlings, with and without the influence of P. fluorescens BBc6. The authors suggested that BBc6 promotes the survival of the fungal inoculum in the soil, since they observed a significant positive bacterial influence on fungal biomass only after autoclaving the nursery soil prior to adding bacteria and fungal inoculum, e.g., under adverse conditions for fungal development (Brulé et al 2001).…”

Section: Modification Of the Mycorrhizosphere Soilmentioning

confidence: 99%

Mycorrhiza Helper Bacteria

Tarkka

Frey‐Klett

2008

Mycorrhiza

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“…According to Garbaye & Duponnois (1993), MHB are not plant-specific, but they are clearly selective in their interactions with various fungal species, being therefore fungus-specific. Because P. fluorescens CECT 844 may be a MHB not specific to Pinus sp., we hypothesize that P. fluorescens CECT 844 as MHB could significantly increase the colonization by T. melanosporum only when the environment (soil) is unsuitable for fungal growth, as suggested by Brule et al (2001) for the fungus Laccaria bicolor. Under unfavorable conditions, it may be also hypothesized that the fungus is not able to prepare a suitable environment to promote mycorrhization, e.g., potentially increasing the mycorrhizal root tips or creating nutritional stress to promote the fungus-plant symbiosis.…”

Section: Seedling Growth and Mycorrhizal Colonizationmentioning

confidence: 91%

The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings

Núñez¹,

Medina²,

Berrocal-Lobo³

et al. 2015

iForest

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© iForest -Biogeosciences and Forestry IntroductionWater and nutrient availability are the main constraints on plant productivity in semi-arid Mediterranean ecosystems. The preservation of mycoflora diversity depends on the status of plant roots (Marulanda et al. 2006). Forest species in these areas often develop specific strategies to improve their water usage in response to drought (Martínez-Ferri et al. 2000).Several studies have been conducted to improve the quality of seedlings produced in nurseries (Caravaca et al. 2005). Several authors reported that soil amendment with ectomycorrhizal fungi and plant-growth-promoting rhizobacteria (PGPR) increased plant survival and seedling quality, especially in soils with low microbial activity (Chanway 1997, Probanza et al. 2001.Pseudomonas fluorescens generally shows several characteristics of an effective PGPR. It is easily cultivated in vitro, and it colonizes a wide range of ecological niches, including plant rhizospheres (Bolton et al. 1993). Additionally, P. fluorescens genomes are highly diverse, which most likely increases the P. fluorescens survival (Silby et al. 2009). The ecological flexibility of such bacteria allows them to exploit a wide variety of nutrients to adapt to environmental changes for survival. P. fluorescens also improves plant growth by producing phytohormones such as auxins (e.g., IAA -Karabaghli et al. 1998). It also has a high capacity for phosphorus solubilization and can produce siderophores (Matthijs et al. 2007). Despite the very well-known positive effects of P. fluorescens on plant survival, only a few studies have been conducted to study its influence on the growth of forest species (Rincón et al. 2008, Ouahmane et al. 2009). We recently demonstrated that the inoculation of Aleppo pine (Pinus halepensis Mill.) with P. fluorescens CECT 844 improved the vegetative growth and N absorption of the P. halepensis seedlings (Dominguez et al. 2012).The use of environmental-friendly natural microbial inocula, such as PGPR or mycorrhizal fungi, is presented in this study as a potential alternative fertilizers. These microorganisms are also beneficial for the maintenance of pre-existing soil microflora, thus contributing to the conservation of soil biodiversity. The amended soil in the nursey increases the vegetative vigor and morphophysiological quality of forest species growth for reforestation purposes (Chanway 1997).Information regarding the productivity of ectomycorrhizal fungi, their ecological functions and their contributions to the productivity and recovery of altered agroecosystems is increasingly valuable in agroforestry. In Spain, the black truffle (Tuber melanosporum Vitt.) is of substantial economic and social value in rural areas of the Mediterranean (Reyna 2007), although studies on the contributions of T. melanosporum to the growth and physiology of forest plants are scarce (Domínguez-Nuñez 2002). Moreover, the ecological value of such symbiosis in the recovery of Mediterranean ecosystems has not been well characterized.The...

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Survival in the soil of the ectomycorrhizal fungus Laccaria bicolor and the effects of a mycorrhiza helper Pseudomonas fluorescens

Cited by 74 publications

References 25 publications

Testing of microbial additives in the rooting of Norway spruce (Picea abies [L.] Karst.) stem cuttings

Testing of microbial additives in the rooting of Norway spruce (Picea abies [L.] Karst.) stem cuttings

Mycorrhiza Helper Bacteria

The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings

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