Survival of Azospirillum Brasilense Under Different Moisture Content Levels

Belaid, Fadel M.; Abubaker, Nagah S.A.; El-Komy, Hesham M. A.

doi:10.53555/eijas.v5i4.111

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…Therefore, an increase in the Azospirillum population with an increase in moisture content (maintained through supplemental irrigation prior to collection of rhizosphere soil samples) was observed in the present study. The same results were reported by Belaid et al [93], who observed that when the moisture content increased, the Azospirillum population increased over time.…”

Section: Microbial Populationsupporting

confidence: 89%

Cumulative Impact of Herbicides and Tillage on the Soil Microbiome, Fungal Diversity and Crop Productivity under Conservation Agriculture

Nthebere,

Tata,

Bhimireddy

et al. 2024

Preprint

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In a diversified cropping system, the tillage methods and weed management practices significantly influence the soil microbiome, which affects crop productivity. The synergetic impacts of such practices on the soil microbiome in association with yield under cotton–maize–Sesbania rostrata rotation with CA have not been extensively explored thus far in southern India. Therefore, a 4-year CA experiment was undertaken to investigate the impact of tillage and weed management on the soil microbiome and fungal diversity at 30 DAS and on the tasselling of maize and crop yield and to identify sustainable tillage and weed management practices that can provide nature-based solutions. The three tillage practices used were T1: CT(C)-CT(M)-fallow (NSr), T2: CT(C)-ZT(M)-ZT(Sr) and T3: ZT+R(C)-ZT+R(M)-ZT+R(Sr), and the following weed control tactics were used: W1-chemical weed control, W2-chemical (herbicide) rotation, W3- integrated weed management (IWM) and the W4-non-weeded control. Rhizosphere soil and rhizoplane samples were collected from the respective plots at 30 DAS after herbicide application and tasselling. Analysis of the microbial population and enzyme and microbial activities, viz., soil basal respiration (SBR), metabolic quotient (qCO2), microbial quotient (qMB), and soil microbial biomass carbon (SMBC) and nitrogen (SMBN), was performed following standard procedures. rRNA gene sequencing of 18S rRNA was performed with rhizosphere soil and rhizoplane fungi isolated at tasselling. The yield was recorded at harvest. The salient findings indicated a decrease in enzyme activity, microbial population, and microbial activity at the initial stage (30 DAS) due to the impact of herbicides, which subsequently increased in response to tasselling, except for qCO2, which decreased. These biological properties were greater in the T3 treatment and nonweeded control followed by IWM, except for qCO2, which showed a decreasing trend relative to T1 and T2 and W1 and W2 at both sampling stages of maize. K yield (KY) and system yield (SY) were greater in the T3, IWM, and herbicide-treated plots (W1 and W2) than in the T1, T2 and nonweeded control plots. Talaromyces flavus, a beneficial rhizosphere soil inhabitant, was identified in T3 in combination with the IWM. Considering both crop productivity and soil biological assessment, T3 and IWM were considered the best treatment combinations among all the other treatments with SY (4453 kg ha-1). These findings signify the importance of adopting reduced tillage (T3) and IWM for farmers while striving for nature-based solutions.

show abstract

Section: Microbial Populationsupporting

confidence: 89%

Cumulative Impact of Herbicides and Tillage on the Soil Microbiome, Fungal Diversity and Crop Productivity under Conservation Agriculture

Nthebere,

Tata,

Bhimireddy

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…So, an increase in the Azospirlium population with an increase in moisture content (maintained through supplemental irrigation prior collection of rhizosphere soil samples) was noticed in the current experiment. The same results were reported by Belaid et al [93] who observed that when moisture content was increased, there was an increase in the Azospirillum population along with time.…”

Section: Microbial Populationsupporting

confidence: 88%

Cumulative Impact of Herbicides and Tillage on Soil Microbiome, Fungal Diversity and Crop Productivity under Conservation Agriculture

Nthebere,

Tata,

Bhimireddy

et al. 2024

Preprint

View full text Add to dashboard Cite

In a diversified cropping system, the kinds of tillage methods and weed management choices adopted exert a significant influence on soil microbiome which has a bearing on crop productivity. The synergetic impacts of such practices on soil microbiome in association with yield under cotton-maize-Sesbania rostrata rotation with CA have not been extensively explored thus far in Southern India. Therefore, a 4-years CA experiment was undertaken to investigate the impact of tillage and weed management on soil microbiome and fungal diversity at 30 DAS and tasselling of maize, crop yield and identify a sustainable tillage and weed management which can provide nature-based solution. Three tillage practices; T1:CT(C)-CT(M)-fallow (NSr), T2:CT(C)-ZT(M)-ZT(Sr) and T3:ZT + R(C)-ZT + R(M)-ZT + R(Sr) and weed control tactics involved; W1-chemical weed control, W2-chemical (herbicide) rotation, W3- integrated weed management (IWM) and W4-non-weeded control laid out in split-plot design. Rhizosphere soil and rhizoplane samples were collected from the respective plots at 30 DAS after herbicides application and tasselling. Analysis for microbial population, enzyme and microbial activities viz., soil basal respiration (SBR), metabolic quotient (qCO2), microbial quotient (qMB), soil microbial biomass carbon (SMBC) and nitrogen (SMBN) was done duly following standard procedures. The rRNA gene sequencing with 18s was performed with rhizosphere soil and rhizoplane fungi isolated at tasselling. Yield was recorded at harvest. The salient findings indicated; a decline in enzyme activities, microbial population, microbial activities at initial stages (30 DAS) due to impact of herbicides which later on increased by tasseling except qCO2 which decreased. These biological properties were higher under T3 and non-weeded control followed by IWM except qCO2 which showed a decreasing trend relative to T1, T2 and W1, W2 at both sampling stages of maize. Kernel yield (KY) and System yield (SY) were enhanced by T3 and IWM, herbicides treated plots (W1 and W2) compared to T1, T2 and non-weeded control. Talaromyces flavus, beneficially rhizosphere soil inhabitant was identified in T3 in combination with IWM. Considering both crop productivity and soil biological assessment, T3 and IWM was considered as best treatment combination among all others with SY (4453 kg ha− 1). These findings signify the importance of adopting reduced tillage (T3) and IWM for the farmer while striving for Nature-based solution.

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“…It was found that the PIA formulation had a higher moisture content than the alginate sample, likely due to PIA being more hydrophilic. There have been several reports which support the impact of residual moisture in improving microbial survival in microcapsules. − …”

Section: Results and Discussionmentioning

confidence: 99%

Encapsulation of Plant Growth-Promoting Bacteria in Poly(itaconic acid) Microspheres by Spray Drying

Kapishon,

Lorrain,

Leung

et al. 2023

ACS Agric. Sci. Technol.

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Poly(itaconic acid) (PIA) is a renewable, biobased, hydrophilic polymer of superabsorbent properties, with the potential to replace synthetic superabsorbent polymers in agricultural applications. Herein, we tested PIA for encapsulation of two model plant growth-promoting bacteria (PGPB), Bacillus subtilis and Pseudomonas fluorescens, using spray drying. Linear PIA was prepared, mixed with PGPB, and spray-dried to form microbe-loaded microspheres. The latter were analyzed for mass yield, moisture content, morphology, and microbial survival. Incubation of the microbes with PIA prior to spray drying showed no significant toxicity. The spray-dried microcapsules were about 1−10 μm in diameter, had a moisture content of about 11%, and contained a viable load of microbes (up to 1 × 10 8 cells/gram of product). The microspheres underwent instantaneous swellingdissolution to release live bacteria. The CFU data showed a survival of about 79% for B. subtilis after spray drying. This work presents PIA as a safe, sustainable, and promising new encapsulation material for microbial inoculants.

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Survival of Azospirillum Brasilense Under Different Moisture Content Levels

Cited by 3 publications

References 2 publications

Cumulative Impact of Herbicides and Tillage on the Soil Microbiome, Fungal Diversity and Crop Productivity under Conservation Agriculture

Cumulative Impact of Herbicides and Tillage on the Soil Microbiome, Fungal Diversity and Crop Productivity under Conservation Agriculture

Cumulative Impact of Herbicides and Tillage on Soil Microbiome, Fungal Diversity and Crop Productivity under Conservation Agriculture

Encapsulation of Plant Growth-Promoting Bacteria in Poly(itaconic acid) Microspheres by Spray Drying

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