Survival of Campylobacter jejuni Strains from Different Origins Under Oxidative Stress Conditions: Effect of Temperature

Garénaux, Amélie; Jugiau, F.; Rama, F.; Jonge, R. de; Denis, M.; Fédérighi, Michel; Ritz, M.

doi:10.1007/s00284-007-9082-8

Cited by 79 publications

(50 citation statements)

References 20 publications

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“…C. jejuni lacks these osmoprotectant systems known to be present in other Gramnegative bacteria and research identifying similar systems that are induced by osmotic stress were not found (Garenaux et al 2008).…”

Section: Osmolaritymentioning

confidence: 98%

Survival mechanisms and culturability of Campylobacter jejuni under stress conditions

Jackson

Davis

Tirado

et al. 2009

Antonie van Leeuwenhoek

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Culture-based isolation and enumeration of bacterial human pathogens from environmental and human food samples has significant limitations.Many pathogens enter a viable but non-culturable(VBNC) state in response to stress, and cannot be detected via culturing methods. Favourable growth conditions with a source of energy and an ideal stoichiometric ratio of carbon to inorganic elements can reverse this VBNC state. This review will focus on the bacterium Campylobacter jejuni which is a leading cause of food borne illness in the developed world. C. jejuni can enter a VBNC state in response to extremes in: pH, moisture content, temperature,nutrient content and salinity. Once in a VBNC state,the organism must maintain an energy balance from substrate oxidation through respiration to grow,divide and remain viable. The goal of this review isa greater understanding of how abiotic stress and thermodynamics influence the viability of C. jejuni.

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Section: Osmolaritymentioning

confidence: 98%

Survival mechanisms and culturability of Campylobacter jejuni under stress conditions

Jackson

Davis

Tirado

et al. 2009

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

show abstract

“…estly thermophilic organism, C. jejuni survives optimally under a 12% CO 2 , 5% O 2 atmosphere at 37 to 42°C (24,61) and is sensitive to a spectrum of conditions, including aerobic, oxidative, heat, and osmotic stresses, as well as nutrient limitation (2,24,43). Many of these conditions are frequently encountered both inside and outside its natural animal hosts.…”

mentioning

confidence: 99%

Atypical Roles for Campylobacter jejuni Amino Acid ATP Binding Cassette Transporter Components PaqP and PaqQ in Bacterial Stress Tolerance and Pathogen-Host Cell Dynamics

et al. 2009

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Campylobacter jejuni is a human pathogen causing severe diarrheal disease; however, our understanding of the survival of C. jejuni during disease and transmission remains limited. Amino acid ATP binding cassette (AA-ABC) transporters in C. jejuni have been proposed as important pathogenesis factors. We have investigated a novel AA-ABC transporter system, encoded by cj0467 to cj0469, by generating targeted deletions of cj0467 (the membrane transport component) and cj0469 (the ATPase component) in C. jejuni 81-176. The analyses described here have led us to designate these genes paqP and paqQ, respectively (pathogenesisassociated glutamine [q] ABC transporter permease [P] and ATPase [Q]). We found that loss of either component resulted in amino acid uptake defects, most notably diminished glutamine uptake. Altered resistance to a series of environmental and in vivo stresses was also observed: both mutants were hyperresistant to aerobic and organic peroxide stress, and while the ⌬paqP mutant was also hyperresistant to heat and osmotic shock, the ⌬paqQ mutant was more susceptible than the wild type to the latter two stresses. The ⌬paqP and ⌬paqQ mutants also displayed a surprising but statistically significant increase in recovery from macrophages and epithelial cells in short-term intracellular survival assays. Annexin V, 4,6-diamidino-2-phenylindole (DAPI), and Western blot analyses revealed that macrophages infected with the ⌬paqP or ⌬paqQ mutant exhibited transient but significant decreases in cell death and extracellular signal-regulated kinase-mitogenactivated protein kinase activation compared to levels in wild-type-infected cells. The ⌬paqP mutant was not defective in either short-term or longer-term mouse colonization, consistent with its increased stress survival and diminished host cell damage phenotypes. Collectively, these results demonstrate a unique correlation of an AA-ABC transporter with bacterial stress tolerances and host cell responses to pathogen infection.

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“…Our study reveals that temperature downshifts up-regulate H. pylori napA expression at mid-log phase, which may increase survival of H. pylori in unfavorable environments. A cross-protection between the cold-shock response and oxidative-stress response might explain this increased resistance at low temperature (Garénaux et al, 2008).…”

Section: Helicobacter Pylori Expression Microarray Temperaturementioning

confidence: 99%

Gene expression profile of Helicobacter pylori in response to growth temperature variation

et al. 2009

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A Helicobacter pylori whole-genome DNA microarray was constructed to study expression profiles of H. pylori in response to a sudden temperature transfer from 37 degrees C to 20 degrees C. The expression level of the genome at each of four time points (15, 30, 60, and 120 min) after temperature downshift was compared with that just before cold treatment. Globally, 10.2 % (n=167) of the total predicted H. pylori genes (n=1636) represented on the microarray were significantly differentially expressed (p<0.05) over a 120 min period after shift to low temperature. The expression profiles of the differentially expressed genes were grouped, and their expression patterns were validated by quantitative real-time PCR. Up-regulated genes mainly included genes involved in energy metabolism and substance metabolism, cellular processes, protein fate, ribosomal protein genes, and hypothetical protein genes, which indicate the compensational responses of H. pylori to temperature downshift. Those genes play important roles in adaption to temperature downshift of H. pylori. Down-regulation of DNA metabolism genes and cell envelope genes and cellular processes genes may reflect damaged functions under low temperature, which is unfavorable to bacterial infection and propagation. Overall, this time-course study provides new insights into the primary response of H. pylori to a sudden temperature downshift, which allow the bacteria to survive and adapt to the new host environment.

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Survival of Campylobacter jejuni Strains from Different Origins Under Oxidative Stress Conditions: Effect of Temperature

Cited by 79 publications

References 20 publications

Survival mechanisms and culturability of Campylobacter jejuni under stress conditions

Survival mechanisms and culturability of Campylobacter jejuni under stress conditions

Atypical Roles for Campylobacter jejuni Amino Acid ATP Binding Cassette Transporter Components PaqP and PaqQ in Bacterial Stress Tolerance and Pathogen-Host Cell Dynamics

Gene expression profile of Helicobacter pylori in response to growth temperature variation

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