Survival of freeze-dried bacteria

Survival of yeasts stored after freeze-drying or liquid-drying

“…By trapping water, extracellular polysaccharides are important for bacterial survival in desiccated natural environments (Billi and Potts, 2002;Potts, 1994). However, our previous study indicated that extracellular polysaccharides of freeze-dried bacteria may trap residual water in the ampoule and decrease the survival rates of the desiccated cells (Miyamoto-Shinohara et al, 2008).…”

“…Our previous research showed that Gram-positive bacteria, which have thick cell walls, generally had higher survival rates after freeze-drying than Gram-negative bacteria, which have thin cell walls (Miyamoto-Shinohara et al, 2008). In S. cerevisiae, hyperosmotic shock induces a change in the organization of the cell wall, apparently resulting from the displacement of periplasmic and cell-wallmatrix material into invaginations of the plasma membrane created by the plasmolysis (Slaninova et al, 2000).…”

“…By plotting the survival curves of freeze-dried species sealed under high vacuum ( 1 Pa) and stored at 5 C in the dark, we previously found that the survival rates of Gram-positive bacteria immediately after freeze-drying and during storage were higher than those of Gram-negative bacteria (Miyamoto-Shinohara et al, 2008). In addition, the survival rate of the yeast Saccharomyces cerevisiae immediately after freeze-drying was lower than those of bacterial species, although S. cerevisiae survival rates were stable during storage (Miyamoto-Shinohara et al, 2000.…”

Are the Soft, Liquid-Like Structures Detected around Bacteria by Ambient Dynamic Atomic Force Microscopy Capsules?

“…The amount of liquid-like substances will be proportional to the concentration of the buffer used. High-resolution imaging of microorganisms in gaseous environments can be of interest in a largely diverse set of fields, such as microbial ultrastructure with nanometer resolution or the study of mechanisms of microbial tolerance to stress conditions, such as desiccation, which is important in food science (9,21,22). However, it has to be emphasized that even though AFM does not impose conditions as aggressive as those of electron microscopy, its results can be strongly affected by other subtle factors, as demonstrated in this work.…”

On-Demand Micro-Power Generation from an Origami-Inspired Paper Biobattery Stack

“…Then, the cells were freeze-dried, keeping them intact through the freezing, sublimation, and desorption processes (Figure 5b). Freeze-drying has been widely used for the long-term preservation of bacterial cells [35,36]. Although not all bacterial strains survive the process, and further studies on exoelectrogens are required, freeze-drying allows the MFC technology to find more applicable and potentially realizable applications as a practical and accessible power supply in remote and resource-constrained environments.…”