Aims: To determine the transfer efficiency of micro-organisms from fomites to hands and the subsequent transfer from the fingertip to the lip. Methods and Results: Volunteers hands were sampled after the normal usage of fomites seeded with a pooled culture of a Gram-positive bacterium (Micrococcus luteus), a Gramnegative bacterium (Serratia rubidea) and phage PRD-1 (Period A). Activities included wringing out a dishcloth ⁄ sponge, turning on ⁄ off a kitchen faucet, cutting up a carrot, making hamburger patties, holding a phone receiver, and removing laundry from the washing machine. Transfer efficiencies were 38AE47% to 65AE80% and 27AE59% to 40AE03% for the phone receiver and faucet, respectively. Transfer efficiencies from porous fomites were <0AE01%. In most cases, M. luteus was transferred most efficiently, followed by phage PRD-1 and S. rubidea. When the volunteers' fingertips were inoculated with the pooled organisms and held to the lip area (Period B), transfer rates of 40AE99%, 33AE97%, and 33AE90% occurred with M. luteus, S. rubidea, and PRD-1, respectively.