Survival of human periodontal ligament cells in media proposed for transport of avulsed teeth

Abstract

Many solutions have been examined as possible storage media for avulsed teeth. In this report, human periodontal ligament (PDL) cells were exposed for 1 h to culture medium, milk, Hanks Balanced Salt Solution (HBSS), Soft Wear, Opti Free, and Solo Care contact lens solutions, Gatorade, and tap water, at room temperature and on ice. The number of viable cells was counted using the trypan blue exclusion technique, immediately after exposure (0 h) and at 24 and 48 h, to test the proliferative capacity of the cells after treatment. The results indicated that a significantly higher number of cells survived and proliferated when the exposures were performed at 0 degrees C. Water had a detrimental effect on the cells, whereas culture medium and HBSS preserved significantly more viable cells than the other experimental solutions. Within the parameters of this study, it appears that HBSS is the optimal storage medium for avulsed teeth. Low-fat milk could serve as an alternative if ice is available. Contact lens solutions or Gatorade on ice could serve as short-term (1 h) storage media if the other solutions are not readily available.

Knowledge of Emergency Management of Avulsed Teeth Among General Dentists in Kathmandu

“…4 If the tooth cannot be replanted within five minutes it should be stored in a medium that will maintain vitality of periodontal ligament fibers. 2,5 It was encouraging to know that 59.8 % of the dentists chose Hank's balanced salt solution (HBSS) as the storage media for an avulsed tooth. Guidelines given by International Association for Dental Traumatology also suggests HBSS as storage media.…”

Medios de almacenamiento y transporte para dientes avulsionados

“…• Agua: La osmolalidad del agua varía de 3 -16 mOsm/Kg y su pH es de 7,41 4,20 es el medio de transporte menos adecuado pues al ser hipotónica desencadena la lisis celular, adicionalmente e l a l m a c e n a m i e n t o e n a g u a más de veinte minutos provoca grandes reabsorciones radiculares 14,20,23,25,27,29 • Saliva: Por tener una osmolalidad de 60 -80 mOsm/Kg y pH de 6,76 -7,35 [12][13][14][15][16]19,20 como por contener bacterias no es muy idónea. No obstante, los estudios demuestran que si el diente se pone en el vestíbulo bucal, los fibroblastos pueden mantenerse vitales unas dos horas.…”

“…El empleo de HBSS ha sido evaluado con éxito y siempre se suele utilizar como control positivo en estudios de medios de almacenamiento. 27 , Renu (BAUSCH AND LOMB) 30 , Titmus (CIBA-VISION) 30 , pero en general refieren que conservan las células del ligamento periodontal por períodos cortos de tiempo.…”

“…3,12 Respecto a la temperatura de transporte, los estudios no son concordantes; pues unos aconsejan conservar en leche a temperatura ambiente (20°C) y otros proponen leche fría (4°C) para mantener l a c a p a c i d a d c l o n o g é n i c a celular. 3,19,21,22,27 Leche evaporada entera 6,1 313 1 25…”

Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames

“…Although there has been some controversy (6,8), it has been suggested that milk with a lower fat content might be more appropriate to maintain PDLF viability than milk with a higher fat content (14). Previous studies indicate that cold milk is more effective than milk at room temperature (3,10,(15)(16)(17). Low temperatures have the advantage of reducing cellular metabolism (18) and limiting bacterial growth (3), which may affect the prognosis of tooth replantation.…”