Survival of Pseudomonas aeruginosa in the Hospital Environment

Hurst, Valerie; Sutter, Vera L.

doi:10.1093/infdis/116.2.151

Cited by 29 publications

(9 citation statements)

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“…Obviously instruments used in these interventions will be highly contaminated. [8] No growth on biopsy instruments could be observed, which can probably be explained by the short duration of the procedure.…”

Section: Discussionmentioning

confidence: 99%

“…For the experimental study, three different bacteria were prepared in sheep blood and used to contaminate sterile stainless steel pieces with surface area of 10cm 2 . After incubation at room temperature for 2, 4,6,8, 12, 24, 36 and 48 h, colonies were counted as compared to time zero. Bacterial counts were between 20 and 320 CFU/cm2, depending on the operation site.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Holding Time on the Bacterial Load of Surgical Instruments

Mohite¹,

S²,

Y³

et al. 2016

jemds

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The aim of this investigation was to determine the bacterial load on used instruments and to evaluate the relationship between the bacterial load and holding time prior to cleaning. Forty six sets were evaluated to establish the average number of bacteria per square centimeter. For the experimental study, three different bacteria were prepared in sheep blood and used to contaminate sterile stainless steel pieces with surface area of 10cm 2 . After incubation at room temperature for 2, 4,6,8, 12, 24, 36 and 48 h, colonies were counted as compared to time zero. Bacterial counts were between 20 and 320 CFU/cm2, depending on the operation site. Bacterial load was found to have increased after 6 hrs. An increase of 3log10 CFU/cm2 was measured after 12 h. It is imperative to clean surgical instruments in the first 6 h to ensure effective disinfection and sterility. KEYWORDSSurgical Instruments, Bacterial Load, Bioburden, Holding Time, Contamination. HOW TO CITE THIS ARTICLE:Mohite ST, Mahesh Reddy S, Kshirsagar AY, et al. Effect of holding time on the bacterial load of surgical instruments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Holding Time on the Bacterial Load of Surgical Instruments

Mohite¹,

S²,

Y³

et al. 2016

jemds

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“…This is due to the fact that the number of pseudomonas in burn wounds can be as high as 10 6 CFU/g tissue. Obviously instruments used in these interventions will be highly contaminated [8]. No growth on biopsy instruments could be observed, which can probably be explained by the short duration of the procedure.…”

Section: Discussionmentioning

confidence: 99%

“…For the experimental study, three different bacteria were prepared in sheep blood and used to contaminate sterile stainless steel pieces with the surface of 10 cm 2 . After incubation at room temperature for 2, 4,6,8, 12, 24, 36, and 48 h, colonies were counted and compared to time zero. Bacterial counts were between 10 and 250 CFU/cm 2 , depending on the operation site.…”

mentioning

confidence: 99%

The Relationship Between Holding Time and the Bacterial Load on Surgical Instruments

et al. 2012

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The aim of this investigation was to determine the bacterial load on used instruments and to evaluate the relationship between the bacterial load and the holding time prior to cleaning. Thirty six sets were evaluated to establish the average number of bacteria per square centimeter. For the experimental study, three different bacteria were prepared in sheep blood and used to contaminate sterile stainless steel pieces with the surface of 10 cm 2 . After incubation at room temperature for 2, 4,6,8, 12, 24, 36, and 48 h, colonies were counted and compared to time zero. Bacterial counts were between 10 and 250 CFU/cm 2 , depending on the operation site. Bacterial load was found to have increased after 6 h. An increase of 3log 10 CFU/cm 2 was measured after 12 h. It is imperative to clean surgical instruments in the first 6 h to ensure effective disinfection and sterility.

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“…This bacterium is well adapting to different environments conditions, such as natural and artifi cial environments, environment with antimicrobial drug, disinfectants or antiseptics. Previously was reported that P.aeruginosa thrives on the moist surfaces, and it can survive more than month on the dry fl oor, on dry fi ler paper until 150 days, in water more than 300 days (Hurst 1966). Due to all this characteristics and reputation for being resistant to disinfection, this opportunistic nosocomial phatogen can cause a wide range of infections, and is a leading cause of illness in immunocompromised individuals.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Hypericum perforatum Essential Oil

Kalabá

Glušac²,

Stijepić³

et al. 2016

QOL

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Abstract:European Centre for Disease Prevention and Control (ECDC) and World Health Organization (WHO) initiate public awareness campaign about antimicrobial substances and their rational uses due to the increasing prevalence of multidrug resistant strains of bacteria. The objective of this study was to evaluate antimicrobial activity of Hypericum perforatum essential oil and reference antimicrobial drugs against the growth of certain bacteria Staphylococcus aureus, Salmonella typhimurium i Pseudomonas aeruginosa.

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Survival of Pseudomonas aeruginosa in the Hospital Environment

Cited by 29 publications

References 0 publications

Effect of Holding Time on the Bacterial Load of Surgical Instruments

Effect of Holding Time on the Bacterial Load of Surgical Instruments

The Relationship Between Holding Time and the Bacterial Load on Surgical Instruments

Antimicrobial Activity of Hypericum perforatum Essential Oil

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