Survival of the fittest: how Brucella strains adapt to their intracellular niche in the host

Roop, R. Martin; Gaines, Jennifer M.; Anderson, Eric S.; Caswell, Clayton C.; Martin, Daniel W.

doi:10.1007/s00430-009-0123-8

Cited by 184 publications

(175 citation statements)

References 187 publications

(253 reference statements)

Supporting

Mentioning

171

Contrasting

Unclassified

Order By: Relevance

“…The conservation of these motifs lends support to the theory first presented in Hallez et al (23) that CtrA mediates cell division through direct regulation of minC transcription in α-proteobacteria containing the Min system and through direct regulation of ftsZ in C. crescentus and other α-proteobacteria that lack the Min system. It is intriguing that a single regulatory factor can retain its role in a process so highly conserved as cell division but simultaneously demonstrates the flexibility to modulate diverse cellular differentiation events central to the lifestyles of α-proteobacteria, which can range from cyst cell formation in Rhodospirillum centenum to intracellular pathogenesis in Brucella (49,50).…”

Section: Discussionmentioning

confidence: 99%

Global analysis of cell cycle gene expression of the legume symbiontSinorhizobium meliloti

Nisco

Abo

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

118

View full text Add to dashboard Cite

In α-proteobacteria, strict regulation of cell cycle progression is necessary for the specific cellular differentiation required for adaptation to diverse environmental niches. The symbiotic lifestyle of Sinorhizobium meliloti requires a drastic cellular differentiation that includes genome amplification. To achieve polyploidy, the S. meliloti cell cycle program must be altered to uncouple DNA replication from cell division. In the α-proteobacterium Caulobacter crescentus, cell cycle-regulated transcription plays an important role in the control of cell cycle progression but this has not been demonstrated in other α-proteobacteria. Here we describe a robust method for synchronizing cell growth that enabled global analysis of S. meliloti cell cycle-regulated gene expression. This analysis identified 462 genes with cell cycle-regulated transcripts, including several key cell cycle regulators, and genes involved in motility, attachment, and cell division. Only 28% of the 462 S. meliloti cell cycle-regulated genes were also transcriptionally cell cycle-regulated in C. crescentus. Furthermore, CtrA-and DnaA-binding motif analysis revealed little overlap between the cell cycle-dependent regulons of CtrA and DnaA in S. meliloti and C. crescentus. The predicted S. meliloti cell cycle regulon of CtrA, but not that of DnaA, was strongly conserved in more closely related α-proteobacteria with similar ecological niches as S. meliloti, suggesting that the CtrA cell cycle regulatory network may control functions of central importance to the specific lifestyles of α-proteobacteria.cell cycle regulation | symbiosis | alpha-proteobacteria

show abstract

Section: Discussionmentioning

confidence: 99%

Global analysis of cell cycle gene expression of the legume symbiontSinorhizobium meliloti

Nisco

Abo

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

118

View full text Add to dashboard Cite

show abstract

“…must survive a range of host-generated stressors (reviewed in Ref. 25). Given our discovery that B. abortus strains lacking an intact GSR system are deficient in survival under acute oxidative and acid stress in vitro (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…(25) and in other bacterial pathogens. To test whether the GSR system plays a role in B. abortus colonization or survival within mammalian cells or an animal host, we infected primary murine macrophages with wild-type B. abortus 2308 and a ⌬rpoE1 mutant and BALB/c mice with wild-type B. abortus 2308, ⌬phyR, and ⌬rpoE1.…”

Section: B Abortus Gsr System Is Required For Maintenance Of Chronicmentioning

confidence: 99%

The Brucella abortus General Stress Response System Regulates Chronic Mammalian Infection and Is Controlled by Phosphorylation and Proteolysis

Kim

Caswell

Foreman

et al. 2013

Journal of Biological Chemistry

Self Cite

119

View full text Add to dashboard Cite

Background: Virulence of pathogenic bacteria is often determined by their ability to adapt to stress. Results: The Brucella abortus general stress response (GSR) system is required for chronic mammalian infection and is regulated by phosphorylation and proteolysis. Conclusion: The B. abortus GSR signaling pathway has multiple layers of post-translational control and is a determinant of chronic infection. Significance: This study provides new, molecular level insight into chronic Brucella infection.

show abstract

“…Brucella bacteria are facultative intracellular pathogens that can survive and replicate within mononuclear phagocytes and within epithelial cells, causing abortion and sterility in domestic animals (Roop et al 2009). Clinical signs of uterine infection are affected by many factors, such as immunity, the number of pathogenic microorganisms, and the uterine environment (Silva et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

Wang

Lin

Yin

et al. 2015

Cell Stress and Chaperones

View full text Add to dashboard Cite

Brucella, which is regarded as an intracellular pathogen responsible for a zoonotic disease called brucellosis, survives and proliferates within several types of phagocytic and non-phagocytic cells. Brucella infects not only their preferred hosts but also other domestic and wild animal species, inducing abortion and infertility. Therefore, the interaction between uterine cells and Brucella is important for understanding the pathogenesis of this disease. In this study, we describe the Brucella suis vaccine strain S2 (B.suis.S2) infection and replication in the immortalized caprine endometrial epithelial cell line hTERT-EECs and the induced cellular and molecular response modulation in vitro. We found that B.suis S2 was able to infect and replicate to high titers and inhibit the proliferation of EECs and induce non-apoptotic pathways, as determined by B.suis.S2 detection using MTT and acridine orange/ethidium bromide (AO/EB) staining and flow cytometry. We explored the evidence of non-apoptotic pathways using real-time quantitative RT-PCR and by western blot analysis. Finally, we discovered the over-expression of GRP78, ATF4, ATF6, PERK, eIF2α, CHOP, and cytochrome c (Cyt-c) but not IRE1, xbp-1, and caspase-3 in B.suis.S2 (HK)-attacked and B.suis.S2-infected cells, suggesting that the molecular mechanism of ER stress sensor activation by B.suis.S2 is basically concomitant with that by B.suis.S2 (HK) and that ER stress, especially the PERK pathway, plays an important role in the process of B.suis.S2 infecting EEC, which may, in part, explain the role of the uterus in the pathogenesis of B.suis.S2.

show abstract

Survival of the fittest: how Brucella strains adapt to their intracellular niche in the host

Cited by 184 publications

References 187 publications

Global analysis of cell cycle gene expression of the legume symbiontSinorhizobium meliloti

Global analysis of cell cycle gene expression of the legume symbiontSinorhizobium meliloti

The Brucella abortus General Stress Response System Regulates Chronic Mammalian Infection and Is Controlled by Phosphorylation and Proteolysis

Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

Contact Info

Product

Resources

About