Survival reduction of Phytophthora capsici oospores and P. nicotianae chlamydospores with Brassica green manures combined with solarization

Lacasa, Carmen María; Martínez, V.; Hernández, A.; Ros, C.; Lacasa, A.; Guerrero, María del Mar; Rodríguez‐Molina, M. C.; Serrano-Pérez, Paula; Larregla, Santiago

doi:10.1016/j.scienta.2015.10.024

Cited by 22 publications

(19 citation statements)

References 56 publications

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“…When Brassica tissues are incorporated into the soil and decomposed, the populations of antagonistic bacteria ( Streptomyces strains) increased, which could decrease the quantity of R . solani [22, 23]. …”

Section: Introductionmentioning

confidence: 99%

Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses

Liu

Zheng

et al. 2017

PLoS ONE

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Greenhouse eggplant monocropping in China has contributed to the aggravation of soil-borne diseases, reductions in crop quality and yield, and the degradation of physical and chemical soil properties. Crop rotation is one effective way of alleviating the problems of continuous cropping worldwide; however, few studies have reported changes in soil bacterial community structures and physical and chemical soil properties after Brassica vegetables had been rotated with eggplant in greenhouses. In this experiment, mustard-eggplant (BFN) and oilseed rape-eggplant (BFC) rotations were studied to identify changes in the physicochemical properties and bacterial community structure in soil that was previously subject to monocropping. Samples were taken after two types of Brassica plants incorporated into soil for 15 days to compare with continually planted eggplant (control, CN) and chemical disinfection of soil (CF) in greenhouses. MiSeq pyrosequencing was used to analyze soil bacterial diversity and structure in the four different treatments. A total of 55,129 reads were identified, and rarefaction analysis showed that the soil treatments were equally sampled. The bacterial richness of the BFC treatment and the diversity of the BFN treatment were significantly higher than those of the other treatments. Further comparison showed that the bacterial community structures of BFC and BFN treatments were also different from CN and CF treatments. The relative abundance of several dominant bacterial genera in the BFC and BFN treatments (such as Flavobacteria, Stenotrophomonas, Massilia and Cellvibrio, which played different roles in improving soil fertility and advancing plant growth) was distinctly higher than the CN or CF treatments. Additionally, the total organic matter and Olsen-P content of the BFC and BFN treatments were significantly greater than the CN treatment. We conclude that Brassica vegetables-eggplant crop rotations could provide a more effective means of solving the problems of greenhouse eggplant monocultures.

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Section: Introductionmentioning

confidence: 99%

Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses

Liu

Zheng

et al. 2017

PLoS ONE

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“…Other microorganisms, including the biocontrol agents Bacillus subtilis and Trichoderma hartianum T39, can prevent the germination of Plasmopara viticola oospores (Vecchione et al, 2005;Dagostin et al, 2006). Glucosinolates and their degradation products, which are generated when Brassica plants are incorporated into soil as green manure, also prevented the germination of Pythium irregulare oospores (Manici et al, 2000) and significantly reduced the viability of oospores of P. capsici when used in combination with solarization (Lacasa et al, 2015). Therefore, the quantification of total oospores (viable and non-viable) may overestimate the inoculum potential in a vineyard.…”

Section: Discussionmentioning

confidence: 99%

A Real-Time PCR Assay for the Quantification of Plasmopara viticola Oospores in Grapevine Leaves

et al. 2020

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Grapevine downy mildew caused by Plasmopara viticola is one of the most important diseases in vineyards. Oospores that overwinter in the leaf litter above the soil are the sole source of inoculum for primary infections of P. viticola; in addition to triggering the first infections in the season, the oospores in leaf litter also contribute to disease development during the season. In the current study, a quantitative polymerase chain reaction (qPCR) method that was previously developed to detect P. viticola DNA in fresh grapevine leaves was assessed for its ability to quantify P. viticola oospores in diseased, senescent grapevine leaves. The qPCR assay was specific to P. viticola and sensitive to decreasing amounts of both genomic DNA and numbers of P. viticola oospores used to generate qPCR standard curves. When the qPCR assay was compared to microscope counts of oospores in leaves with different levels of P. viticola infestation, a strong linear relationship (R 2 = 0.70) was obtained between the numbers of P. viticola oospores per gram of leaves as determined by qPCR vs. microscopic observation. Unlike microscopic observation, the qPCR assay was able to detect significant differences between leaf samples with a low level of oospore infestation (25% infested leaves and 75% noninfested leaves) vs. samples without infestation, and this ability was not influenced by the weight of the leaf sample. The results indicate that the qPCR method is sensitive and provides reliable estimates of the number of P. viticola oospores in grapevine leaves. Additional research is needed to determine whether the qPCR method is useful for quantifying P. viticola oospores in grapevine leaf litter.

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“…Glucosinolate hydrolysis is dependent upon a multitude of factors, including development stage, 36,51 maceration efficiency 52,53 and several soil characteristics. 23,56,57,60 Therefore, this research focused on ITC production of macerated plant tissues without external amelioration. Both experiments yielded promising results for brown mustard, which caused significant F. graminearum and F. poae growth reduction in vitro and antagonized the adverse effects of Fusarium infection on maize in pots.…”

Section: Discussionmentioning

confidence: 99%

“…Covering the soil after incorporation further reduces aerial losses 58 . Other soil parameters that play a role are temperature, 59, 60 organic matter, 61 pH 23 and microbial catabolism 56, 62 . This complex combination of factors has made it a challenge to yield promising results in pot and field trials, despite the proven toxic effect of ITCs and Brassicaceae crops in vitro 52, 63–65 .…”

Section: Introductionmentioning

confidence: 99%

Uncovering the biofumigant capacity of allyl isothiocyanate from several Brassicaceae crops against Fusarium pathogens in maize

et al. 2020

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BACKGROUND: Driven by environmental concerns, chemical fumigants are no longer allowed in many countries. Therefore, other strategies for reducing fungal inoculum in soils and on crop debris are being explored. In the present study, several Brassicaceae crops were screened for their potential to control Fusarium gramineaum and Fusarium poae mycelial growth in an in vitro inverted Petri dish experiment. Volatile production was measured using gas chromatography-mass spectrometry headspace analysis. A selection of cultivars from each crop species was further investigated using a pot experiment with maize. RESULTS: Ethiopian mustard (Brassica carinata) and brown mustard (Brassica juncea) released volatile allyl isothiocyanate (AITC) and a higher concentration of AITC was correlated with a better fungal growth reduction in the in vitro screening. Brown mustard cultivar Etamine completely inhibited growth of both Fusarium spp. Pure AITC in a solution with methanol resulted in a sigmoid dose-response curve for both Fusarium spp. tested. Fusarium poae appeared to be more tolerant to AITC than F. graminearum. A pot experiment revealed that the incorporation of brown mustard plant material could alleviate the clear negative effect of F. graminearum infection on maize growth. CONCLUSION: The present study demonstrated the correlation between the fungistatic effect of biofumigation crops on Fusarium spp. and their production of volatile AITC in vitro, without the addition of exogenous enzymes, and confirmed the biofumigation potential of brown mustard in a pot experiment with maize. These results may help farmers when selecting a green manure crop suitable for biofumigation.

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Survival reduction of Phytophthora capsici oospores and P. nicotianae chlamydospores with Brassica green manures combined with solarization

Cited by 22 publications

References 56 publications

Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses

Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses

A Real-Time PCR Assay for the Quantification of Plasmopara viticola Oospores in Grapevine Leaves

Uncovering the biofumigant capacity of allyl isothiocyanate from several Brassicaceae crops against Fusarium pathogens in maize

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