Surviving at the edge of a fragmented range: patterns of genetic diversity in isolated populations of the endangered giant Mediterranean limpet (Patella ferruginea)

Cossu, Piero; Scarpa, Fabio; Dedola, Gian Luca; Sanna, Daria; Lai, Tiziana; Cristo, Benedetto; Curini‐Galletti, Marco; Panzalis, Pieraugusto; Navone, Augusto; Careddu, G.M.; Congiatu, Pier Paolo; Mura, L.; Fois, Nicola

doi:10.1007/s00227-017-3080-6

Cited by 12 publications

(30 citation statements)

References 93 publications

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“…All the above would indicate that the planktonic larval period of P. ferruginea could be extended enough to allow its dispersion over distances larger than initially thought (Templado et al, 2018;Ferranti et al, 2019). This would explain in part the genetic uniformity observed throughout most of its distribution range (Espinosa & Ozawa, 2006;Casu et al, 2012;Acevedo et al, 2018), with only some exceptions for local populations in some MPAs of Sardinia described by Cossu et al (2017). In this regard, current increasing sea temperatures due to climate change may affect the larval phase and thus the dispersal ability and population connectivity of Patella ferruginea and other invertebrate species.…”

Section: Larval Developmentmentioning

confidence: 87%

Filling gaps: closing the life cycle of the endangered Mediterranean limpet Patella ferruginea Gmelin, 1791 (Gastropoda, Patellidae)

et al. 2020

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Several reproductive issues and the larval development of the ferruginous limpet, Patella ferruginea Gmelin, 1791, an endangered species endemic from the western Mediterranean Sea, were studied to fill gaps in the knowledge of its life cycle.Average diameter of mature oocytes was 141.83 μm and mean oocyte density in the ovary was 283,800 oocytes/gram. No significant correlations were found between both oocyte diameter or density and female shell length. Female fecundity (number of oocytes per gonad) was significantly correlated with shell length and varied between 189,200 oocytes in a 40.0 mm female and 5,019,200 oocytes in an 86.4 mm female. However, there was considerable variability, in particular for largest females.Spawning induction was not achieved using usual molluscan aquaculture methods. Thus, oocytes obtained after dissection of females were used for fertilizations trials. Alkalinization treatments of seawater were used to test improvement in oocyte maturation and later fertilization rates. Treatment at pH 9 during 2 h produced the highest increase in the percentage of mature oocytes and in the fertilization rate; but these results showed high variability and were mainly significant when the initial degree of maturation was low. Sperm concentration experiments determined that best in vitro fertilization were performed at 105 and 5×105 spermatozoids/ml. The sequence and timing of the complete larval development of Patella ferruginea in laboratory conditions is described and illustrated here for the first time. At 20 °C, larvae became competent for metamorphosis 3 days after fertilization, but some crawling pediveliger larvae with a still well developed velum were found even 7 days after fertilization. Recruits 1-2 mm in length were achieved in low numbers from two of the last assays and were first detected between 131-141 days after fertilization. The resulting juveniles were monitored during two years and sex determination of five survivors at the end of this period showed that two were mature males, two mature females and one indeterminate.Our results show that the main reproductive traits or larval development of P. ferruginea hardly differ from those of other non-endangered Mediterranean or NE Atlantic limpet species. Therefore, its decline cannot be mainly attributed to some constraints of these traits as was previously suggested, but to human impact.On the other hand, it is feasible to complete the life cycle of this species in laboratory conditions, from fertilized eggs to mature individuals. However, an important part of the process like spawning induction was not achieved as gonads needed to be dissected fatally from females, although sperm could be obtained from males through non-lethal biopsies. At present, large-scale aquaculture production for reintroduction, restocking or stock enhancement purposes is neither possible nor an advisable conservation tool yet. Further study is required and meanwhile, an appropriate design of a network of effectively protected marine areas that ensures connectivity among extant populations is necessary.

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Section: Larval Developmentmentioning

confidence: 87%

Filling gaps: closing the life cycle of the endangered Mediterranean limpet Patella ferruginea Gmelin, 1791 (Gastropoda, Patellidae)

et al. 2020

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“…This short planktonic phase suggests that gene flow may be present only at small geographic scales (Casu et al 2006). The relatively high genetic diversity within populations in 2 natural marine protected areas (MPAs) in the north of Sardinia provides supporting evidence (Cossu et al 2017). On the other hand, Casu et al (2011) identified 2 main genetic groups within the Mediterranean and hypothesised that gene flow may occur between populations from the Straits of Gibraltar to Sicily.…”

Section: Conservation Status Ecology and Traitsmentioning

confidence: 87%

‘Impossible’ re-introduction of the endangered limpet Patella ferruginea Gmelin, 1791? Progress in resolving translocation mortality

Fa¹,

Finlayson²,

Sempere-Valverde³

et al. 2018

Endang. Species. Res.

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“…Regarding genetic diversity, overall, the heterozygosity at nuclear loci of Tritia neritea in its native range (0.486 < He < 0.724, Table 1 ) is comparable to that of other gastropods 66 – 68 , although lower than the values for another direct developing gastropod species Crepidula convexa 52 (0.885 < He < 0.943) or the broadcast spawner C. fornicata 69 (0.806 < He < 0.838). Additionally, all the F is values are high and significant, demonstrating a heterozygote deficiency in every populations.…”

Section: Discussionmentioning

confidence: 82%

Chaotic genetic structure and past demographic expansion of the invasive gastropod Tritia neritea in its native range, the Mediterranean Sea

Boissin

Neglia

Baksay

et al. 2020

Sci Rep

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To better predict population evolution of invasive species in introduced areas it is critical to identify and understand the mechanisms driving genetic diversity and structure in their native range. Here, we combined analyses of the mitochondrial COI gene and 11 microsatellite markers to investigate both past demographic history and contemporaneous genetic structure in the native area of the gastropod Tritia neritea, using Bayesian skyline plots (BSP), multivariate analyses and Bayesian clustering. The BSP framework revealed population expansions, dated after the last glacial maximum. The haplotype network revealed a strong geographic clustering. Multivariate analyses and Bayesian clustering highlighted the strong genetic structure at all scales, between the Black Sea and the Adriatic Sea, but also within basins. Within basins, a random pattern of genetic patchiness was observed, suggesting a superimposition of processes involving natural biological effects (no larval phase and thus limited larval dispersal) and putative anthropogenic transport of specimens. Contrary to the introduced area, no isolation-by-distance patterns were recovered in the Mediterranean or the Black Seas, highlighting different mechanisms at play on both native and introduced areas, triggering unknown consequences for species’ evolutionary trajectories. These results of Tritia neritea populations on its native range highlight a mixture of ancient and recent processes, with the effects of paleoclimates and life history traits likely tangled with the effects of human-mediated dispersal.

show abstract

Surviving at the edge of a fragmented range: patterns of genetic diversity in isolated populations of the endangered giant Mediterranean limpet (Patella ferruginea)

Cited by 12 publications

References 93 publications

Filling gaps: closing the life cycle of the endangered Mediterranean limpet Patella ferruginea Gmelin, 1791 (Gastropoda, Patellidae)

Filling gaps: closing the life cycle of the endangered Mediterranean limpet Patella ferruginea Gmelin, 1791 (Gastropoda, Patellidae)

‘Impossible’ re-introduction of the endangered limpet Patella ferruginea Gmelin, 1791? Progress in resolving translocation mortality

Chaotic genetic structure and past demographic expansion of the invasive gastropod Tritia neritea in its native range, the Mediterranean Sea

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