Susceptibilidad antimicrobiana y bases genéticas de la resistencia de cepas de Enterococcus causantes de infecciones en Cuba

Pérez, Dennis; Capote, Miriam Abreu; Marrero, Deisy; Alvarez, Ana Bertha; Ortiz, Cecilia; Salomé, Francisco; Llop, Alina; Campo, Rosa del

doi:10.1590/s1020-49892011001200009

Cited by 4 publications

(6 citation statements)

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“…In a previous recent report from nosocomial infections surveillance in the "Octavio de la Concepción y la Pedraja" Hospital from Cuba, enterococci have been identified as an important nosocomial pathogen causing bacteremias, urinary tract infections, wound infections, and other clinical pediatric infections [8]. Here we presented data about molecular determinants of antibiotic resistance and clonal relationship of E. faecalis isolates that had caused infections in this pediatric hospital.…”

Section: Discussionmentioning

confidence: 83%

“…Most isolates were collected from pediatric intensive care unit (PICU) (18%), surgery ward (16.3%), community (16.3%), neonatology ward (14.5%) and urology ward (14.5%). Clinical diagnosis of patients from both hospitalized and non-hospitalized children were as follows: surgical wounds infections, urinary tract infections, septicemia, otitis media, abscesses, vaginitis, catheter tip infection, endotracheal tube infection, meningitis and other infections [1,3,5,[8][9][10].…”

Section: Methodsmentioning

confidence: 99%

“…In a recent report from infectious disease surveillance in a pediatric hospital in Cuba, the circulation of enterococcal strains and their virulence traits and antibiotic resistance patterns were studied [8]. However, genetic relationship of these isolates to the antimicrobial resistance remained unknown.…”

Section: Genesmentioning

confidence: 99%

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Genetic diversity and antibiotic resistance determinants of Enterococcus faecalis isolates causing pediatric infections in Cuba

Quiñones

Marrero

Llop

et al. 2015

J Pediatr Infect Dis

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Genetic relationships and the antibiotic resistance determinants were studied for a total of 55 Enterococcus faecalis isolates recovered from children in a pediatric hospital in Cuba between January 2001 and December 2004. These isolates were analyzed by pulsed-field gel electrophoresis, and presence of some resistance genes to aminoglycosides, macrolides and tetracycline were examined by polymerase chain reaction. A total of 17 pulsed-field gel electrophoresis patterns were identified among 54 different patterns of pulsed-field gel electrophoresis. Particular multi-resistant E. faecalis clones were detected in different wards from the pediatric hospital. The aac(6')-aph(2") and aph(3') genes was detected in all the isolates with highlevel resistance to gentamicin and amikacin, respectively, whereas the ant(6) gene was detected in the 75% of isolates showing high-level resistance to streptomycin. The erm(B) gene was found in 72% of the erythromycin-resistant strains, and tet(M) was detected in all tetracycline resistant strains. The circulation of particular clones exhibiting antibiotic resistance in the pediatric hospital and the dissemination of drug-resistance genes among these strains indicated the need for the monitoring of antibiotic susceptibility to prevent the selection or dispersion of resistant strains in this hospital.

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Section: Discussionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

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Genetic diversity and antibiotic resistance determinants of Enterococcus faecalis isolates causing pediatric infections in Cuba

Quiñones

Marrero

Llop

et al. 2015

J Pediatr Infect Dis

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“…Para el caso del medicamento de referencia (Norfloxacina) se evaluaron concentraciones desde 0,10 hasta 5,1 × 10 6 mg/mL. 24 A cada tubo de ensayo se agregaron 100µl de la solución estándar de E. faecalis ATCC 29212. Se incluyeron dos grupos controles uno con solución salina como control de crecimiento y otro con diluciones seriadas de etanol desde 96% a 0,18% para descartar el posible efecto del solvente.…”

Section: Revistaunclassified

“…32 La norfloxacina es un medicamento de estructura química conocida cuyo mecanismo de acción ha sido ampliamente estudiado y que fue purificado para su uso en esta investigación. 24 Las evidencias experimentales presentan la posibilidad de aplicación y uso del propóleo en diversas especialidades de la odontología y coinciden en resaltar los amplios beneficios que podría tener su uso terapéutico en la cavidad bucal. Todo esto soportado en el hecho de ser una sustancia natural a la que se le atribuyen efectos antimicrobianos, analgési-cos, antiinflamatorios y cicatrizantes.…”

Section: Revista Ustasaludunclassified

Actividad Antimicrobiana in Vitro De Un Propóleo De Santander Sobre Enterococcus Faecalis

et al. 2012

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RESUMENObjetivo: evaluar la actividad antimicrobiana de un extracto etanólico de propóleo de Santander sobre Enterococcus faecalis. Materiales y métodos: se realizó un estudio experimental In vitro. A partir de un propóleo crudo del municipio de Lebrija (Santander), se obtuvo un extracto etanólico a través del método Soxhlet. La actividad antimicrobiana se determinó con una prueba de macrodilución en tubo y recuento de Unidades Formadoras de Colonias (UFC) por mL luego de 48 horas. Se evaluaron concentraciones de propóleo desde 100 hasta 0,19 mg/mL, como control se incluyeron diluciones del disolvente (etanol 96%) y se usó como medicamento de referencia la norfloxacina. Resultados: se determinó que concentraciones de propóleo de 100 mg/mL y 50 mg/mL inhibieron 100% de crecimiento de E. faecalis. El extracto de propóleo mostró Concentración Inhibitoria 50 (CI 50 ) de 1,19 mg/mL y Concentración Inhibitoria 90 (CI 90 ) de 7,92 mg/ mL. Mientras que la norfloxacina fue más efectiva con CI 50 y CI 90 de 0,06 y 2,39 µg/mL. El etanol no mostró efecto antimicrobiano a las concentraciones presentes en el extracto. Conclusión: con el apoyo de estudios complementarios, es posible proponer al propóleo santandereano como una alternativa de irrigación y medicación en endodoncia, dado el potente efecto frente a E. faecalis siendo este el patógeno endodóntico más frecuente en infecciones persistentes. IN VITRO ANTIMICROBIAL ACTIVITY OF ETANOLIC PROPOLIS EXTRACTS FROM SANTANDER AGAINST Enterococcus faecalisABSTRACT Objective: to evaluate the antimicrobial activity of an ethanolic propolis extract from Santander province against Enterococcus faecalis. Methods: an experimental In vitro study was made. Starting from pristine propolis collected in the city of Lebrija (Santander), an ethanolic extract was obtained using a Soxhlet apparatus. The antimicrobial activity was measured using the macro dilution tube technique by counting the Colony Forming Units (CFU) per mL after 48 hours. Propolis solutions with concentrations in the range from 100 to 0.19 mg/mL were evaluated. Tubes containing dilutions of the antibiotic norfloxacin and solvent (96% ethanol) were used as reference and control. Results: the propolis solutions within the range from 100 mg/mL to 50 mg/mL inhibited 100% growing of E. faecalis. Propolis extract showed an inhibitory concentration 50 (IC 50 ) of 1.19 mg/mL and an inhibitory concentration 90 (IC 90 ) of 7.92 mg/mL respectively. Norfloxacin on the other hand was more effective with IC 50 and IC 90 of 0.06 and 2.39 µg/mL respectively. Ethanol showed non-antimicrobial activity at the concentration present within the extracts. Conclusion: with further studies support it would be possible to suggest the use of propolis from Santander as an alternative of irrigation and medication in endodontics because of its powerful effect against E. faecalis considering it is the most frequent pathogen found in persistent infections.

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Distribution of acquired antibiotic resistance genes among Enterococcus spp. isolated from a hospital in Baotou, China

Tian

Wang

2019

BMC Res Notes

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ObjectiveThis study investigated the distribution of acquired antibiotic resistance genes in Enterococcus species isolated from clinical patients in Baotou, China.ResultA total of 73 enterococca lisolates from clinical samples were collected from December 2016 to September 2017. Of the 73 enterococcal isolates, 36 (49.3%), 35 (47.9%), 1 (1.4%), and 1 (1.4%) were identified as E. faecium, E. faecalis, E. gallinarum, and E. raffinosus, respectively. The resistance rates of the enterococci to nitrofurantoin, tetracycline, gentamicin (high-level), ampicillin, ciprofloxacin and erythromycin were 24.7%, 49.3%, 50.7%, 54.8%, 74.0% and 89.0%, respectively. The most prevalent aminoglycoside resistance genes were aac(6′)-Ie-aph(2″)-Ia (64.9%) and aph(3′)IIIa (64.9%). The most common erythromycin ribosome methylation gene was erm(B) (67.7%), followed by erm(A) (4.6%) and erm(C) (1.5%). The tetracycline resistance gene tetM was found to be present in 100.0% of the tetracycline-resistant strains of enterococci. Thus, E. faecium and E. faecalis were identified as the species of greatest clinical importance associated with hospital-acquired enterococcal infections in Baotou, China. The antimicrobial resistance genes aac(6′)-Ie-aph(2″)-Ia, aph(3′)IIIa, tetM, and erm(B) were significantly more prevalent among the enterococcal isolates. Therefore, action should be taken to monitor drug resistance and antimicrobial resistance genes to manage multi-drug-resistant enterococcal infections.Electronic supplementary materialThe online version of this article (10.1186/s13104-019-4064-z) contains supplementary material, which is available to authorized users.

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Susceptibilidad antimicrobiana y bases genéticas de la resistencia de cepas de Enterococcus causantes de infecciones en Cuba

Cited by 4 publications

References 24 publications

Genetic diversity and antibiotic resistance determinants of Enterococcus faecalis isolates causing pediatric infections in Cuba

Genetic diversity and antibiotic resistance determinants of Enterococcus faecalis isolates causing pediatric infections in Cuba

Actividad Antimicrobiana in Vitro De Un Propóleo De Santander Sobre Enterococcus Faecalis

Distribution of acquired antibiotic resistance genes among Enterococcus spp. isolated from a hospital in Baotou, China

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