Susceptibility of Dermanyssus gallinae from China to acaricides and functional analysis of glutathione S-transferases associated with beta-cypermethrin resistance

Wang, Chuanwen; Xu, Xiaolin; Huang, Yu; He, Yang; Li, Hao; Wan, Qiang; Li, Huan; Wang, Lianyu; Sun, Yanyan; Pan, Baoliang

doi:10.1016/j.pestbp.2020.104724

Cited by 20 publications

(9 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dg‐fer1 and Dg‐fer2 were knocked down separately by RNAi in D. gallinae as described previously 29 . Diethyl pyrocarbonate (DEPC)‐treated water was used as a blank control and enhanced green fluorescent protein (EGFP) was used as nontarget control.…”

Section: Methodsmentioning

confidence: 99%

“…Dg-fer1 and Dg-fer2 were knocked down separately by RNAi in D. gallinae as described previously. 29 Diethyl pyrocarbonate (DEPC)-treated water was used as a blank control and enhanced green fluorescent protein (EGFP) was used as nontarget control. Dg-fer1, Dg-fer2 and EGFP genes were amplified by PCR using primers containing the T7 RNA polymerase promoter (TAAT ACGACTCACTATAGG) (Table 1).…”

Section: Rna Interference (Rnai)mentioning

confidence: 99%

See 1 more Smart Citation

Two ferritins from Dermanyssus gallinae: characterization and in vivo assessment as protective antigens

Wang

Liu

et al. 2021

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND: The poultry red mite, Dermanyssus gallinae is recognized worldwide as the most important bloodsucking ectoparasite in layer and breeder flocks. In bloodsucking ectoparasites, ferritins (FERs), the iron-storage proteins, play a pivotal role in dealing with the challenge of large amounts of released iron during the digestion of blood meal. However, no information is available concerning FERs of D. gallinae. The aim of the present study was to investigate the characteristics, functions and the vaccine efficacy of FERs in D. gallinae.RESULTS: Two heavy-chain FERs of D. gallinae were identified and characterized. Phylogenetic analysis indicated that Dg-FER1 may be a secretory FER and Dg-FER2 an intracellular one. RNAi results demonstrated that Dg-fers play key roles in mite survival, successful reproduction and blood digestion. Immunization with rDg-FER1 or rDg-FER2 successfully induced chickens to produce high levels of antigen-specific IgY, resulting in a significant increase in mite mortality (by 58.67% on Day 5) and decreases in oviposition (by 42.15%) and fecundity (by 68.97%) in the rDg-FER1 group, and a 13.73% increase in mite mortality and a 20.89% decrease in fecundity in the rDg-FER1 group. The overall immunization efficacy of rDg-FER1 was 93.51%. CONCLUSION: Two Dg-FERs are crucial to the survival, reproduction and blood digestion of D. gallinae. This study has provided preliminary evidence demonstrating the potential of rDg-FER1 as a vaccine antigen for D. gallinae.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Rna Interference (Rnai)mentioning

confidence: 99%

Two ferritins from Dermanyssus gallinae: characterization and in vivo assessment as protective antigens

Wang

Liu

et al. 2021

Pest Management Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chemical acaricides in the environment have been widely applied [215,218,225]. However, a widespread resistance to such molecules has been recorded in the last few decades [218,[226][227][228][229][230]; moreover, many acaricides have been withdrawn from the European market [218]. These drugs, in fact, would have a public health impact, occurring as residues in eggs and meat [231,232].…”

Section: Dermanyssus Gallinaementioning

confidence: 99%

Entomopathogenic Fungi and Bacteria in a Veterinary Perspective

Ebani

Mancianti

2021

Biology

View full text Add to dashboard Cite

The present study aimed to review the papers dealing with the biological activity of fungi and bacteria against some mites and ticks of veterinary interest. In particular, the attention was turned to the research regarding acarid species, Dermanyssus gallinae and Psoroptes sp., which are the cause of severe threat in farm animals and, regarding ticks, also pets. Their impact on animal and human health has been stressed, examining the weaknesses and strengths of conventional treatments. Bacillus thuringiensis, Beauveria bassiana and Metarhizium anisopliae are the most widely employed agents. Their activities have been reviewed, considering the feasibility of an in-field application and the effectiveness of the administration alone or combined with conventional and alternative drugs is reported.

show abstract

“…Such an approach is particularly timely as new tools to understand gene function (e.g. gene silencing by RNAi) have recently been developed and optimised in PRM [16,43,99] and some limited transcriptomic and bioinformatics studies on differential gene expression in PRM in different physiological states are starting to emerge (e.g. [3,30,41]).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the poultry red mite, Dermanyssus gallinae, across all stages of the lifecycle

et al. 2021

View full text Add to dashboard Cite

Background The blood feeding poultry red mite (PRM), Dermanyssus gallinae, causes substantial economic damage to the egg laying industry worldwide, and is a serious welfare concern for laying hens and poultry house workers. In this study we have investigated the temporal gene expression across the 6 stages/sexes (egg, larvae, protonymph and deutonymph, adult male and adult female) of this neglected parasite in order to understand the temporal expression associated with development, parasitic lifestyle, reproduction and allergen expression. Results RNA-seq transcript data for the 6 stages were mapped to the PRM genome creating a publicly available gene expression atlas (on the OrcAE platform in conjunction with the PRM genome). Network analysis and clustering of stage-enriched gene expression in PRM resulted in 17 superclusters with stage-specific or multi-stage expression profiles. The 6 stage specific superclusters were clearly demarked from each other and the adult female supercluster contained the most stage specific transcripts (2725), whilst the protonymph supercluster the fewest (165). Fifteen pairwise comparisons performed between the different stages resulted in a total of 6025 Differentially Expressed Genes (DEGs) (P > 0.99). These data were evaluated alongside a Venn/Euler analysis of the top 100 most abundant genes in each stage. An expanded set of cuticle proteins and enzymes (chitinase and metallocarboxypeptidases) were identified in larvae and underpin cuticle formation and ecdysis to the protonymph stage. Two mucin/peritrophic-A salivary proteins (DEGAL6771g00070, DEGAL6824g00220) were highly expressed in the blood-feeding stages, indicating peritrophic membrane formation during feeding. Reproduction-associated vitellogenins were the most abundant transcripts in adult females whilst, in adult males, an expanded set of serine and cysteine proteinases and an epididymal protein (DEGAL6668g00010) were highly abundant. Assessment of the expression patterns of putative homologues of 32 allergen groups from house dust mites indicated a bias in their expression towards the non-feeding larval stage of PRM. Conclusions This study is the first evaluation of temporal gene expression across all stages of PRM and has provided insight into developmental, feeding, reproduction and survival strategies employed by this mite. The publicly available PRM resource on OrcAE offers a valuable tool for researchers investigating the biology and novel interventions of this parasite.

show abstract

Susceptibility of Dermanyssus gallinae from China to acaricides and functional analysis of glutathione S-transferases associated with beta-cypermethrin resistance

Cited by 20 publications

References 50 publications

Two ferritins from Dermanyssus gallinae: characterization and in vivo assessment as protective antigens

Two ferritins from Dermanyssus gallinae: characterization and in vivo assessment as protective antigens

Entomopathogenic Fungi and Bacteria in a Veterinary Perspective

Transcriptomic analysis of the poultry red mite, Dermanyssus gallinae, across all stages of the lifecycle

Contact Info

Product

Resources

About