Susceptibility of different grapevine (Vitis vinifera L.) cultivars to Diplodia seriata and Diplodia mutila

Ramı́rez, Mario; Pérez, Luz; Montealegre, J.

doi:10.7764/rcia.v45i1.1818

Cited by 14 publications

(12 citation statements)

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“…B. dothidea is a pathogen associated with grapevine decline in Iran [ 47 ], cankers on eucalypts in China [ 48 ] and with the fruit rot of apples in Italy [ 49 ]; while D. mutila was found as causal agent of the trunk diseases of several cultivars of grapevines in Chile [ 36 ], canker and dieback of apple trees in China [ 50 ] and the leaf blight of Magnolia grandiflora L. in China [ 51 ]. Here, we identified B. dothidea and D. mutila as seedborne pathogens able to compromise seed germination as well as causing seedling blight.…”

Section: Discussionmentioning

confidence: 99%

“…Pathogenicity tests were carried out by inoculating the stem of P. vulgaris . The seeds of the landrace Dente di Morto were sown in sterile soil and grown in a climatic chamber under a photoperiod of 12 h. After 4 weeks, the plants were inoculated with CREA OF 360.4 and CREA OF 420.36 using the V-shaped cut method, previously described by Ramirez et al [ 36 ]. Briefly, a V-shaped cut was done on the plant stem and a plug of the mycelium was applied.…”

Section: Methodsmentioning

confidence: 99%

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Identification and Characterization of New Seedborne Pathogens in Phaseolus vulgaris Landraces of Southern Italy

et al. 2023

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The diagnostic survey of seedborne fungal pathogens is fundamental for symptomless material stored in gene banks to avoid the diffusion of pathogens by germplasm distribution and propagation. In this work, seeds of Southern Italian landraces of the common bean (Phaseolus vulgaris L.) belonging to the gene bank at CREA (Italy) were inspected to assess their phytosanitary status. The phytopathological analysis revealed the presence of the most common pathogens associated with common bean seeds such as Fusarium spp., Macrophomina phaseolina, Rhizoctonia solani, Colletotrichum lindemuthianum and Diaporthe/Phomopsis complex. However, new fungi able to completely inhibit seed germination were also observed. The most aggressive were isolated, and the morpho-pathological characterization, DNA sequencing and phylogenetic analysis allowed us to define the strains as Botryosphaeria dothidea CREA OF 360.4 and Diplodia mutila CREA OF 420.36. These two plant pathogens are generally associated with grapevines and other fruit trees. Pathogenicity tests were carried out along with a transmissibility test in which the transmission of the pathogens to the seedlings was proven. Host range experiments revealed the ability of these pathogens to infect crops such as pepper and melon. To our knowledge, this is the first time that B. dothidea and D. mutila were detected on the common bean.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Identification and Characterization of New Seedborne Pathogens in Phaseolus vulgaris Landraces of Southern Italy

et al. 2023

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“…Likewise, wood necrosis was more extensive in canes of Merlot and Pinot Noir than in Chardonnay, Cabernet Sauvignon and Sauvignon Blanc inoculated with N. australe , N. luteum and N. parvum (Billones‐Baaijens et al 2014). Ramírez et al (2018) reported Cabernet Franc, Syrah (Shiraz) and Sauvignon Blanc to have significantly more wood necrosis than Merlot and Malbec following inoculation of canes with D. mutila , although there was no difference among cultivars inoculated with D. seriata .…”

Section: Introductionmentioning

confidence: 99%

Winegrape cultivars ( Vitis vinifera ) vary in susceptibility to the grapevine trunk pathogens Eutypa lata and Diplodia seriata

Sosnowski

Ayres

McCarthy

et al. 2021

Aust J Grape and Wine Res

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Background and Aims Eutypa and Botryosphaeria dieback are diseases that affect vineyards worldwide, reducing productivity and longevity. The ability of the causal pathogens Eutypa lata and Diplodia seriata to colonise grapevine canes was investigated in the search for resistance or tolerance to trunk diseases. Methods and Results Visual assessments of 174 winegrape cultivars (Vitis vinifera) in a mature germplasm collection in the Barossa Valley in South Australia revealed substantial variation (0–98%) in severity of foliar symptoms and dieback. A selection of 18 cultivars representing those without symptoms or low (<10%) severity was compared with the susceptible cultivars Shiraz and Sauvignon Blanc. Inoculation of canes in situ in the vineyard and detached canes in the greenhouse, confirmed variability among cultivars for the extent of pathogen colonisation. Conclusions Cultivars varied in susceptibility to colonisation by E. lata and D. seriata, with some differences between the two pathogens in terms of the ranking of cultivars. Muscadelle was most resistant to colonisation by both pathogens, and Sauvignonasse was the most susceptible. Significance of the Study Knowledge of cultivar susceptibility to grapevine trunk diseases will assist in prioritisation of disease management strategies. Identification of cultivars with resistance could inform selection of planting material and may lead to improved breeding programs in the future.

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“…S. sclerotiorum CREA OF 1113.1 was inoculated on 3-week-old broad bean plants (local variety “Lunga”) by the V-shape method [ 91 ], which consists in cutting the plant stem and applying mycelium plugs of 10-day-old colonies on the wounds. Sterile mycelium was used as a negative control.…”

Section: Methodsmentioning

confidence: 99%

Surveillance of Landraces’ Seed Health in South Italy and New Evidence on Crop Diseases

Dell’Olmo

Zaccardelli

Sanajà

et al. 2023

Plants

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During the last three years, more than 300 landraces belonging to different plant species have been the main focus of an Italian valorization research project (AgroBiodiversità Campana, ABC) aiming at analyzing, recovering, preserving, and collecting local biodiversity. In this context, phytosanitary investigation plays a key role in identifying potential threats to the preservation of healthy seeds in gene banks and the successful cultivation of landraces. The surveillance carried out in this study, in addition to highlighting the expected presence of common species-specific pathogens such as Ascochyta pisi in peas, Ascochyta fabae in broad beans, and Macrophomina phaseolina, Xanthomonas axonopodis pv. phaseoli, and Xanthomonas fuscans subsp. fuscans in beans, pointed to the presence of novel microorganisms never detected before in the seeds of some hosts (Apiospora arundinis in common beans or Sclerotinia sclerotiorum and Stemphylium vesicarium in broad beans). These novel seedborne pathogens were fully characterized by (i) studying their morphology, (ii) identifying them by molecular methods, and (iii) studying their impact on adult crop plants. For the first time, this study provides key information about three novel seedborne pathogens that can be used to correctly diagnose their presence in seed lots, helping prevent the outbreaks of new diseases in the field.

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Susceptibility of different grapevine (Vitis vinifera L.) cultivars to Diplodia seriata and Diplodia mutila

Cited by 14 publications

References 10 publications

Identification and Characterization of New Seedborne Pathogens in Phaseolus vulgaris Landraces of Southern Italy

Identification and Characterization of New Seedborne Pathogens in Phaseolus vulgaris Landraces of Southern Italy

Winegrape cultivars ( Vitis vinifera ) vary in susceptibility to the grapevine trunk pathogens Eutypa lata and Diplodia seriata

Surveillance of Landraces’ Seed Health in South Italy and New Evidence on Crop Diseases

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