Susceptibility of human enterotoxigenic<i>Escherichia coli</i>isolates to growth inhibition by porcine intestinal epithelial cells

Brown, E. A.; Kaushik, Radhey S.; Hardwidge, Philip R.

doi:10.1111/j.1574-6968.2007.00814.x

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…These authors also noted that polarization of the IPEC-J2 cells did not affect the binding patterns compared to the same cells when not polarized. Additionally, some strains of human-derived ETEC, but not porcine-derived ETEC, seem to be inhibited by a heat-stable factor that is secreted by IPEC-J2 cells (Brown et al, 2007). This factor was only generated by the cells when they were grown in medium supplemented with serum, and it seemed to be specific for ETEC, as the attachment of multiple enterohemorrhagic E. coli (EHEC) O157:H7 strains remained unaffected.…”

Section: Introductionmentioning

confidence: 99%

Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations

Brosnahan

Brown

2012

Veterinary Microbiology

165

138

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IPEC-J2 cells are porcine intestinal columnar epithelial cells that were isolated from neonatal piglet mid-jejunum. This cell line forms polarized monolayers with high transepithelial electrical resistance when cultured on 0.4 μm pore-size filters. The cell line is unique in that it is derived from small intestinal tissue (compared to the common human colon-derived lines HT-29, T84, and Caco-2) and is not transformed (compared to the porcine small intestinal line, IPI-2I). Porcine intestinal epithelial cells more closely mimic human physiology than analogous rodent cell lines (e.g. IEC-6 or IEC-18), which is important in studies of zoonotic infections; in addition, they provide specificity to study porcine-derived infections. IPEC-J2 cells are increasingly being used in microbiological studies to examine the interactions of various animal and human pathogens, including Salmonella enterica and pathogenic Escherichia coli, with intestinal epithelial cells. The IPEC-J2 cell line has also been employed in some probiotic studies, in which the cells have been used as an initial screening tool for adhesiveness and anti-inflammatory properties of the potential probiotic microorganisms. The validity of these studies is not clear as follow-up studies to assess the efficacy of the probiotics in vivo have not been published to date. The aims of this review are to provide a comprehensive overview of the microbiological studies that have been conducted with IPEC-J2 cells and a reference guide of key cellular and immune markers that have been identified in this cell line that may prove to be useful in future studies.

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Section: Introductionmentioning

confidence: 99%

Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations

Brosnahan

Brown

2012

Veterinary Microbiology

165

138

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“…The two types of small intestinal cells available for ETEC adherence studies are the IPEC-1 and IPEC-J2 cell lines. These unique cells derived from 1-day-old piglets, either from the small intestine (IPEC-1) or specifically from the jejunum (IPEC-J2), have provided valuable insights on ETEC interactions with the human intestinal mucosa (Brown et al, 2007;Geens & Niewold, 2010;Johnson et al, 2009;Koh et al, 2008;Schierack et al, 2006). Pig intestinal cells closely resemble human intestinal cells in physiology and may express receptors for ETEC CSs, including CS21.…”

Section: Introductionmentioning

confidence: 99%

Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

et al. 2013

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Colonization surface antigens (CSs) represent key virulence-associated factors of enterotoxigenic Escherichia coli (ETEC) strains. They are required for gut colonization, the first step of the diarrhoeal disease process induced by these bacteria. One of the most prevalent CSs is CS21, or longus, a type IV pili associated with bacterial self-aggregation, protection against environmental stresses, biofilm formation and adherence to epithelial cell lines. The objectives of this study were to assess the role of CS21 in adherence to primary intestinal epithelial cells and to determine if CS21 contributes to the pathogenesis of ETEC infection in vivo. We evaluated adherence of a CS21-expressing wild-type ETEC strain and an isogenic CS21-mutant strain to pig-derived intestinal cell lines. To determine the role of CS21 in pathogenesis we used the above ETEC strains in a neonatal mice challenge infection model to assess mortality. Quantitative adherence assays confirmed that ETEC adheres to primary intestinal epithelial cells lines in a CS21-dependent manner. In addition, the CS21-mediated ETEC adherence to cells was specific as purified LngA protein, the CS21 major subunit, competed for binding with the CS21-expressing ETEC while specific anti-LngA antibodies blocked adhesion to intestinal cells. Neonatal DBA/2 mice died after intra-stomach administration of CS21-expressing strains while lack of CS21 expression drastically reduced the virulence of the wild-type ETEC strain in this animal model. Collectively these results further support the role of CS21 during ETEC infection and add new evidence on its in vivo relevance in pathogenesis.

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Integrated microfluidic device to monitor unseen Escherichia coli contamination in mammalian cell culture

Dervisevic

Ang

et al. 2022

Sensors and Actuators B: Chemical

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Susceptibility of human enterotoxigenicEscherichia coliisolates to growth inhibition by porcine intestinal epithelial cells

Cited by 3 publications

References 35 publications

Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations

Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations

Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

Integrated microfluidic device to monitor unseen Escherichia coli contamination in mammalian cell culture

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