Susceptibility of Isofamilies of<i>Spodoptera frugiperda</i>(Lepidoptera: Noctuidae) to Cry1Ac and Cry1Fa Proteins of<i>Bacillus thuringiensis</i>

Blanco, Carlos A.; Portilla, Maribel; Jurat-Fuentes, Juan Luís; Sánchez, J Fernández; Viteri, Diego M.; Vega-Aquino, Paulina; Terán-Vargas, Antonio P.; Azuara-Domínguez, Ausencio; López, J. D.; Arias, Renée S.; Zhu, Yu‐Cheng; Lugo-Barrera, David; Jackson, R. E.

doi:10.3958/059.035.0325

Cited by 91 publications

(65 citation statements)

References 26 publications

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“…Larvae from the 456 strain were reported to be highly resistant to purified Cry1Fa, Cry1Ac, and Cry1Ab toxins (12,13,25). In agreement with these observations, Ͻ10% mortality was detected in the 456 strain in bioassays with leaf tissue from transgenic corn expressing the Cry1Fa toxin (Fig.…”

Section: Resultssupporting

confidence: 76%

“…While nonrecessive (56) and dominant (57) resistance alleles have also been detected in field populations of H. armigera from China, their relevance to Cry toxin binding has not been evaluated. Our data and previous reports (5,10,25) provide support for Cry1Fa-resistant S. frugiperda from Puerto Rico, fulfilling the assumption of monogenic resistance transmitted as a recessive autosomal trait, considered in insect resistance management (IRM) practices for Bt crops (58). The lack of significant differences in susceptibility or Cry1Fa toxin binding between homozygous susceptible and heterozygous larvae observed in this study is analogous to Cry1Ac resistance in strains of H. virescens linked to mutations in cadherin genes (19).…”

Section: Discussionsupporting

confidence: 57%

“…The goal of this study was to identify the biochemical mechanism responsible for field-evolved resistance to corn event TC1507 in a strain of S. frugiperda (456) initiated from insects collected from corn fields in Puerto Rico (25). Our data provide a mechanistic description of field resistance to TC1507 corn and support the idea of ALP proteins being putative Cry1Fa toxin receptors, whose altered expression is associated with reduced Cry1Fa toxin binding in the midguts of resistant S. frugiperda larvae.…”

mentioning

confidence: 69%

“…The 456 strain of S. frugiperda was generated from a cross of two moths as part of a set of multiple single-pair crossings of insects obtained from corn fields in the Santa Isabel region of Puerto Rico. Before being submitted to selection, larvae of the 456 strain displayed high levels of field-evolved resistance to Cry1Fa (Ͼ7,500-fold) and Cry1Ac (Ͼ40-fold) toxins compared to those of susceptible S. frugiperda strains from diverse geographies (25). Larvae of the 456 and Ben strains were reared in the laboratory for about 24 generations and selected for four generations until they reached the third-instar stage on fresh corn leaf tissue from event TC1507 (for 456) or plants from TC1507's nontransgenic isoline, 2T777 (for Ben), before completion of the assays.…”

Section: Insectsmentioning

confidence: 99%

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Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression

Jakka

Gong

Hasler³

et al. 2016

Appl Environ Microbiol

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dInsecticidal protein genes from the bacterium Bacillus thuringiensis (Bt) are expressed by transgenic Bt crops (Bt crops) for effective and environmentally safe pest control. The development of resistance to these insecticidal proteins is considered the most serious threat to the sustainability of Bt crops. Resistance in fall armyworm (Spodoptera frugiperda) populations from Puerto Rico to transgenic corn producing the Cry1Fa insecticidal protein resulted, for the first time in the United States, in practical resistance, and Bt corn was withdrawn from the local market. In this study, we used a field-collected Cry1Fa corn-resistant strain (456) of S. frugiperda to identify the mechanism responsible for field-evolved resistance. Binding assays detected reduced Cry1Fa, Cry1Ab, and Cry1Ac but not Cry1Ca toxin binding to midgut brush border membrane vesicles (BBMV) from the larvae of strain 456 compared to that from the larvae of a susceptible (Ben) strain. This binding phenotype is descriptive of the mode 1 type of resistance to Bt toxins. A comparison of the transcript levels for putative Cry1 toxin receptor genes identified a significant downregulation (>90%) of a membrane-bound alkaline phosphatase (ALP), which translated to reduced ALP protein levels and a 75% reduction in ALP activity in BBMV from 456 compared to that of Ben larvae. We cloned and heterologously expressed this ALP from susceptible S. frugiperda larvae and demonstrated that it specifically binds with Cry1Fa toxin. This study provides a thorough mechanistic description of field-evolved resistance to a transgenic Bt crop and supports an association between resistance and reduced Cry1Fa toxin binding and levels of a putative Cry1Fa toxin receptor, ALP, in the midguts of S. frugiperda larvae.

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Section: Resultssupporting

confidence: 76%

Section: Discussionsupporting

confidence: 57%

mentioning

confidence: 69%

Section: Insectsmentioning

confidence: 99%

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Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression

Jakka

Gong

Hasler³

et al. 2016

Appl Environ Microbiol

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“…Nevertheless, some populations of S. frugiperda evolved resistance to cry1F (Matten et al 2008, Tabashnik et al 2009, Storer et al 2010, Blanco et al 2010 and possible cross-resistance to Cry1Ab and Cry1Ac (Hernández-Rodríguez et al 2013). However, proteins such as Cry1Aa, Cry1Ab, and Cry1Ac have been ineffective (van Rie et al 1990, Aranda et al 1996, Luttrell et al 1999, Santos et al 2009, Storer et al 2010, but have great activity against other insect species (Bravo et al 1998, Martínez & Caballero 2002, Patel et al 2009.…”

Section: Resultsmentioning

confidence: 99%

Cry Proteins from Bacillus thuringiensis Active against Diamondback Moth and Fall Armyworm

et al. 2015

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Biopesticides based on Bacillus thuringiensis and genetically modified plants with genes from this bacterium have been used to control Plutella xylostella (L.) and Spodoptera frugiperda (J.E. Smith). However, the selection pressure imposed by these technologies may undermine the efficiency of this important alternative to synthetic insecticides. Toxins with different modes of action allow a satisfactory control of these insects. The purpose of this study was to characterize the protein and gene contents of 20 B. thuringiensis isolates from soil and insect samples collected in several areas of Northeast Brazil which are active against P. xylostella and S. frugiperda. Protein profiles were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polymerase chain reaction assays were used to determine toxin genes present within bacterial isolates. The protein profile of the majority of the isolates produced bands of approximately 130 kDa, suggesting the presence of Cry1, Cry8 and Cry9 proteins. The gene content of the isolates of B. thuringiensis investigated showed different gene profiles. Isolates LIIT-4306 and LIIT-4311 were the most actives against both species, with LC50 of 0.03 and 0.02 × 10(8) spores mL(-1), respectively, for P. xylostella, and LC50 of 0.001 × 10(8) spores mL(-1) for S. frugiperda. These isolates carried the cry1, cry1Aa, cry1Ab, cry1Ac, cry1B, cry1C, cry1D, cry1F, cry2, cry2A, cry8, and cry9C genes. The obtained gene profiles showed great potential for the control of P. xylostella and S. frugiperda, primarily because of the presence of several cry1A genes, which are found in isolates of B. thuringiensis active against these insects.

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Identification of entomopathogenic fungus Metarhizium rileyi infested in fall armyworm in the cornfield of Korea, and evaluation of its virulence

Acharya

Lee

Hwang

et al. 2022

Arch Insect Biochem Physiol

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The fall armyworm (FAW) Spodoptera frugiperda is an important invasive pest in Africa and Asia. It is a polyphagous pest with at least 353 recorded host plant species, including corn. Chemical control of this pest is unsuccessful because of a developed resistance and harmful effects on the environment. Entomopathogenic fungi are potential biological control agents for FAW. In this study, the native strain of Metarhizium rileyi (KNU‐Ye‐1), collected from a cornfield at Yeongcheon, Korea, was identified by morphological and molecular characterization. The susceptibility of the fourth‐instar larvae of FAW to the native strain M. rileyi was examined in the laboratory. The results showed that the Korean strain of M. rileyi (KNU‐Ye‐1) was highly virulent to FAW larvae, causing 89% mortality 7 days posttreatment. Therefore, M. rileyi (KNU‐Ye‐1) identified in this study is highly valuable for the biological control of FAW in the field.

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Susceptibility of Isofamilies ofSpodoptera frugiperda(Lepidoptera: Noctuidae) to Cry1Ac and Cry1Fa Proteins ofBacillus thuringiensis

Cited by 91 publications

References 26 publications

Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression

Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression

Cry Proteins from Bacillus thuringiensis Active against Diamondback Moth and Fall Armyworm

Identification of entomopathogenic fungus Metarhizium rileyi infested in fall armyworm in the cornfield of Korea, and evaluation of its virulence

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