Susceptibility of juvenile sole Solea senegalensis to marine isolates of viral haemorrhagic septicaemia virus from wild and farmed fish

López-Vázquez, Carmen; Conde, MV González; Dopazo, Carlos P.; Barja, Juan L.; Bandı́n, Isabel

doi:10.3354/dao02299

Cited by 12 publications

(11 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although no viral haemorrhagic septicaemia virus (VHSV) outbreaks have been reported for the time being, López‐Vazquez et al . () demonstrated the susceptibility of Senegalese sole to a VHSV strain isolated from wild Greenland halibut, Reinhardtius hippoglossoides , and farmed turbot. On the other hand, in wild fish stocks which were held as future breeders, the presence of intracytoplasmic inclusion bodies in erythrocytes was detected causing low but continuous mortality (Padrós et al .…”

Section: Health Issuesmentioning

confidence: 99%

New developments and biological insights into the farming of Solea senegalensis reinforcing its aquaculture potential

Morais

Aragão

Cabrita

et al. 2014

Reviews in Aquaculture

119

108

View full text Add to dashboard Cite

Senegalese sole was one of the earliest identified candidate species with high potential for aquaculture diversification in the south of Europe. Its culture has been possible, and commercially attempted, for several decades, but intensive production has been slow to take off. This has been explained mostly by serious disease problems, high mortality at weaning, variable growth and poor juvenile quality. However, a strong and sustained research investment that started in the eighties has led to a better understanding of the requirements and particularities of this species. More recently, better management and technical improvements have been introduced, which have led to important progress in productivity and given a new impetus to the cultivation of Senegalese sole. As a result, the last 5 years have marked a probable turning point in the culture of sole towards the development of a knowledge-driven, competitive and sustainable industry. This review will focus on the main technical improvements and advances in the state of knowledge that have been made in the last decade in areas as diverse as reproductive biology, behaviour, physiology, nutritional requirements, modulation of the immune system in response to environmental parameters and stress, and characterization and mitigation of the main disease threats. It is now clear that Senegalese sole has important particularities that differentiate it from other current and candidate marine aquaculture species, which bring about important challenges, some still unsolved, but also notable opportunities (e.g. a nutritional physiology that is better adapted to dietary vegetable ingredients), as will be discussed here.

show abstract

Section: Health Issuesmentioning

confidence: 99%

New developments and biological insights into the farming of Solea senegalensis reinforcing its aquaculture potential

Morais

Aragão

Cabrita

et al. 2014

Reviews in Aquaculture

119

108

View full text Add to dashboard Cite

show abstract

“…Furthermore, VHSV has been recently detected in wild fish caught in southern European coastal waters [ 25 ]. Therefore, the existence of this marine VHSV reservoir may represent a potential risk for farmed Senegalese sole, which has been demonstrated to be susceptible to VHSV by experimental infection [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Role of the IFN I system against the VHSV infection in juvenile Senegalese sole (Solea senegalensis)

Álvarez-Torres

Podadera

Béjar

et al. 2016

Vet Res

View full text Add to dashboard Cite

Senegalese sole is susceptible to marine VHSV isolates but is not affected by freshwater isolates, which may indicate differences regarding virus-host immune system interaction. IFN I induces an antiviral state in fish, stimulating the expression of genes encoding antiviral proteins (ISG). In this study, the stimulation of the Senegalese sole IFN I by VHSV infections has been evaluated by the relative quantification of the transcription of several ISG (Mx, Isg15 and Pkr) after inoculation with marine (pathogenic) and freshwater (non-pathogenic) VHSV isolates. Compared to marine VHSV, lower levels of RNA of the freshwater VHSV induced transcription of ISG to similar levels, with the Isg15 showing the highest fold induction. The protective role of the IFN I system was evaluated in poly I:C-inoculated animals subsequently challenged with VHSV isolates. The cumulative mortality caused by the marine isolate in the control group was 68%, whereas in the poly I:C-stimulated group was 5%. The freshwater VHSV isolate did not cause any mortality. Furthermore, viral RNA fold change and viral titers were lower in animals from the poly I:C + VHSV groups than in the controls. The implication of the IFN I system in the protection observed was confirmed by the transcription of the ISG in animals from the poly I:C + VHSV groups. However, the marine VHSV isolate exerts a negative effect on the ISG transcription at 3 and 6 h post-inoculation (hpi), which is not observed for the freshwater isolate. This difference might be partly responsible for the virulence shown by the marine isolate.

show abstract

“…These data seem to indicate that although VHSV can infect sole via waterborne exposure as we had previously reported (Lopez‐Vazquez et al . ), this process is poorly efficient, probably due to the protection conferred by non‐specific immune mechanisms at epidermal and epithelial fish surfaces (Yamamoto et al . ; Dorson & Torhy ).…”

Section: Discussionmentioning

confidence: 99%

“…VHSV SM2897 (genotype III), obtained from farmed turbot and demonstrated to be moderately pathogenic to sole (Lopez‐Vazquez et al . ), was the isolate used in several challenges together with IPNV SpSse‐IAusc4028. Both strains were propagated on bluegill fry (BF‐2) cells grown in minimum essential medium (MEM, Thermo Fisher Scientific) plus 10% foetal bovine serum (Lonza) with 100 IU mL −1 penicillin and 0.1 mg mL −1 streptomycin (Lonza).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

In vivo study of viral haemorrhagic septicaemia virus and infectious pancreatic necrosis virus coexistence in Senegalese sole (Solea senegalensis)

López-Vázquez

Alonso

Dopazo

et al. 2016

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

The effect of IPNV-VHSV coinfection and superinfection on the mortality caused by both viruses in Senegalese sole has been analysed. No effect was observed after coinfection. However, a clear viral interference was recorded between a primary IPNV and a subsequent VHSV infection, which led to a survival increase in the infected sole of 50% points when compared with fish infected only with VHSV. The significantly higher Mx transcriptional values in the fish pre-exposed to IPNV (at least at first days after superinfection) and the increased daily mortality when low Mx transcriptional levels were recorded suggest that Mx may be involved in the protective effect against VHSV infection. However, in fish subjected to VHSV primary/IPNV secondary infection, no interference was observed.

show abstract

Susceptibility of juvenile sole Solea senegalensis to marine isolates of viral haemorrhagic septicaemia virus from wild and farmed fish

Cited by 12 publications

References 22 publications

New developments and biological insights into the farming of Solea senegalensis reinforcing its aquaculture potential

New developments and biological insights into the farming of Solea senegalensis reinforcing its aquaculture potential

Role of the IFN I system against the VHSV infection in juvenile Senegalese sole (Solea senegalensis)

In vivo study of viral haemorrhagic septicaemia virus and infectious pancreatic necrosis virus coexistence in Senegalese sole (Solea senegalensis)

Contact Info

Product

Resources

About