Susceptibility of laboratory rats against genotypes 1, 3, 4, and rat hepatitis E viruses

Li, Tiancheng; Yoshizaki, Sayaka; Ami, Yasushi; Suzaki, Yuriko; Yasuda, Shumpei P.; Yoshimatsu, Kumiko; Arikawa, Jiro; Takeda, Naokazu; Wakita, Takaji

doi:10.1016/j.vetmic.2012.12.014

Cited by 45 publications

(54 citation statements)

References 39 publications

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“…Similar procedures have been described for human, pig, rabbit and avian HEV cDNA clones (Emerson et al, 2001;Cossaboom et al, 2014;Huang et al, 2005a, b). Nude rats were selected for inoculation as they have been shown to be highly susceptible to rat HEV infection, leading to shedding of high titres of rat HEV for a prolonged time (Li et al, 2013b). The detection of rat HEV RNA in serum and faeces after inoculation of the RNA into the liver of the nude rats was the first indication of the successful generation of infectious virus.…”

Section: Discussionmentioning

confidence: 99%

“…The hepatotropism of rat HEV was also confirmed with laboratory rats intravenously inoculated with rat HEVcontaining organ homogenates (Li et al, 2013b). Immunodeficient nude rats inoculated with rat HEV developed persistent infections with prolonged shedding of high amounts of virus (Li et al, 2013b). Experimentally infected Wistar rats showed transient virus shedding and developed anti-rat HEV-specific antibodies (Li et al, 2013b).…”

Section: Introductionmentioning

confidence: 88%

“…Immunodeficient nude rats inoculated with rat HEV developed persistent infections with prolonged shedding of high amounts of virus (Li et al, 2013b). Experimentally infected Wistar rats showed transient virus shedding and developed anti-rat HEV-specific antibodies (Li et al, 2013b). Clinical signs were not recorded after experimental infection of rats (Li et al, 2013b;Purcell et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Experimentally infected Wistar rats showed transient virus shedding and developed anti-rat HEV-specific antibodies (Li et al, 2013b). Clinical signs were not recorded after experimental infection of rats (Li et al, 2013b;Purcell et al, 2011). Preliminary infection trials with rat-derived cell cultures did not result in rat HEV replication (Johne et al, 2010a).…”

Section: Introductionmentioning

confidence: 99%

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Construction and characterization of an infectious cDNA clone of rat hepatitis E virus

Yang

Yoshizaki

et al. 2015

Journal of General Virology

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Rat hepatitis E virus (HEV) is related to human HEV and has been detected in wild rats worldwide. Here, the complete genome of rat HEV strain R63/DEU/2009 was cloned downstream of the T7 RNA polymerase promoter and capped genomic RNA generated by in vitro transcription was injected into nude rats. Rat HEV RNA could be detected in serum and faeces of rats injected intrahepatically, but not in those injected intravenously. Rat HEV RNA-positive faecal suspension was intravenously inoculated into nude rats and Wistar rats leading to rat HEV RNA detection in serum and faeces of nude rats, and to seroconversion in Wistar rats. In addition, rat HEV was isolated in PLC/PRF/5 cells from the rat HEV RNA-positive faecal suspension of nude rats and then passaged. The cell culture supernatant was infectious for nude rats. Genome analysis identified nine point mutations of the cell-culture-passaged virus in comparison with the originally cloned rat HEV genome. The results indicated that infectious rat HEV could be generated from the cDNA clone. As rats are widely used and well-characterized laboratory animals, studies on genetically engineered rat HEV may provide novel insights into organ tropism, replication and excretion kinetics as well as immunological changes induced by hepeviruses.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Construction and characterization of an infectious cDNA clone of rat hepatitis E virus

Yang

Yoshizaki

et al. 2015

Journal of General Virology

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show abstract

“…The fecal specimens were diluted with 10 mM phosphate-buffered saline to obtain 10z suspensions (16). RNA was extracted from the diluted fecal suspensions using MagNA Pure LC Total Nucleic Acid isolation kit (Roche Applied Science, Mannheim, Germany) according to the manufacturer's recommendations.…”

Section: Discussionmentioning

confidence: 99%

Ferret Hepatitis E Virus Infection in Japan

Yonemitsu

Terada

et al. 2015

Jpn J Infect Dis

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SUMMARY:We examined 85 fecal samples from pet ferrets in 10 animal hospitals in Japan for the detection of ferret hepatitis E virus (HEV) RNA. We found that 6 (7.1z) of the samples were positive for ferret HEV RNA. Phylogenetic analysis based on the partial ORF1 indicated that these ferret HEV strains were clearly separated from the Netherlands strains and were divided into 2 distinct clusters. These results suggest that ferret HEV is genetically diverse, and since ferrets are not indigenous to Japan, ferret HEV has been introduced into Japan through importation.

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Hepatitis E Virus

Meng¹

2020

The Liver

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Susceptibility of laboratory rats against genotypes 1, 3, 4, and rat hepatitis E viruses

Cited by 45 publications

References 39 publications

Construction and characterization of an infectious cDNA clone of rat hepatitis E virus

Construction and characterization of an infectious cDNA clone of rat hepatitis E virus

Ferret Hepatitis E Virus Infection in Japan

Hepatitis E Virus

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