Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode

Costa, Anna Carolina Borges Pereira; Chibebe, José; Pereira, Cristiane Aparecida; Machado, Ana Karina da Silva; Beltrame, Milton; Junqueira, Juliana Campos; Jorge, Antônio Olavo Cardoso

doi:10.1590/s1806-83242010000400007

Cited by 61 publications

(41 citation statements)

References 18 publications

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“…Several researchers have reported that PDT is capable of killing oral bacteria in planktonic cultures [21][22][23] and biofilm conditions [13,14]. If bacteria within dentine carious lesions can be eliminated by PDT, it might have positive consequences for dental health.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

et al. 2013

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Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals that are capable of inducing cell death. The present study evaluated the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to PDT grown as multi-species in the biofilm phase versus in dentine carious lesions. A brainheart infusion culture medium supplemented with 1 % glucose, 2 % sucrose, and 1 % young primary culture of L. acidophilus 10 8 CFU/mL and S. mutans 10 8 CFU/mL was used to develop multi-species biofilms and to induce caries on human dentine slabs. Five different concentrations of curcumin (0.75, 1.5, 3.0, 4.0, and 5.0 g/L) were used associated with 5.7 J/cm 2 light emission diode. Four different groups were analyzed L−D− (control group), L−D+ (drug group), L+D− (light group), and L+D+ (PDT group). ANOVA/Tukey's tests were conducted to compare groups. A significant reduction (p <0.05) in cell viability was observed in the biofilm phase following photosensitization with all curcumin concentrations tested. To achieve significant bacterial reduction (p <0.05) in carious dentine, it was necessary to utilize 5.0 g/L of curcumin in association with blue light. No significant reduction was found for L−D+, supporting the absence of the drug's dark toxicity. S. mutans and L. acidophilus were susceptible to curcumin in the presence of blue light. However, due to light penetration and drug diffusion difficulties, these microorganisms within dentine carious lesions were less affected than they were in the biofilm phase.

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Section: Discussionmentioning

confidence: 99%

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

et al. 2013

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“…Similar results have been shown in previous studies using different photosensitizers on oral bacteria. [38][39][40][41] One of the main observations made during the first tests to photoinactivate bacteria with conventional photosensitizers was the relative sensitivity of gram-positive strains to photodynamic inactivation. 24 This sensitivity was confirmed in our study.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Araújo

Fontana

Bagnato

et al. 2012

Photomedicine and Laser Surgery

101

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Background data: The presence of Streptococcus mutans and Lactobacillus acidophilus in dental structure is an indicator of a cariogenic biofilm. Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals capable of inducing cell death. Reduction of bacteria levels can provide additional means of preventing dental caries. Objective: The present study evaluated the susceptibility of planktonic cultures of S. mutans (ATCC 25175) and L. acidophilus (ATCC-IAL-523) from the Adolfo Lutz Institute (IAL) to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: Bacterial suspensions of S. mutans and L. acidophilus isolated (as single species) and combined (multspecies) were prepared and then evaluated. Four different groups were analyzed: L-D-(control group), L-D + (drug group), L + D-(light group), and L + D + (photodynamic therapy group). Two different concentrations of curcumin were tested (0.75 and 1.5 g/L) associated with a 5.7 J/cm 2 light emission diode. Results: Significant decreases ( p < 0.05) in the viability of S. mutans were only observed when the bacterial suspensions were exposed to both curcumin and light. Then, reductions in viability of up to 99.99% were observed when using 1.5 g/L of the photosensitizer. The susceptibility of L. acidophilus was considerably lower (21% and 37.6%) for both curcumin concentrations. Conclusions: Photodynamic therapy was found to be effective in reducing S. mutans and L. acidophilus on planktonic cultures. No significant reduction was found for L-D + , proving the absence of dark toxicity of the drug.

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“…And the reduction rate in the isolated S. mutans appeared to be similar or slightly lower than the reference strain. Carolina et al 32) has verified the effect of photodynamic therapy with erythrosine using a LED on planktonic cultures of ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688). The results showed that PDT with erythrosine exerted an antimicrobial effect on all S. mutans strains studied.…”

Section: ⅳ Discussionmentioning

confidence: 99%

The Effect of Photodynamic Therapy on the Viability of Streptococcus Mutans Isolated From Oral Cavity

Jung¹,

Park²,

Lee

et al. 2012

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizer by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. The aim of this study was to evaluate the effect of PDT on Streptococcus mutans in planktonic conditions, previously treated with different photosensitive concentrations of erythrosine, using halogen and LED curing unit as a light source. And we compared the effects of PDT on six strains of S. mutans isolated from oral cavity and reference strain.As a result, S. mutans was susceptible to the combination of hand held photopolymerizer (HHP) and erythrosine. The higher concentration of erythrosine in the presence of light irradiation induced greater effects in reduction of viability of S. mutans. Isolated S. mutans showed a significant reduction in bacterial counts of the groups submitted to PDT compared to the control groups. And they appeared to be similar or slightly lower antimicrobial effect compared with reference strain. However, the difference was not significant (p < 0.05).In conclusion, PDT using erythrosine as a photosensitizing agent and HHP as a light source could be an efficient option for diseases caused by S. mutans.

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Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode

Cited by 61 publications

References 18 publications

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

The Effect of Photodynamic Therapy on the Viability of Streptococcus Mutans Isolated From Oral Cavity

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