Susceptibility of two strains of mice to the infection with Schistosoma mansoni: Parasitological and biochemical studies

Dajem, Saad M. Bin; Mostafa, Osama; Elsaid, Fahmy G.

doi:10.1007/s00436-008-1092-3

Cited by 19 publications

(16 citation statements)

References 36 publications

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“…The male worms' proportion was higher than the female worms' proportion in both treated and non-treated mice; this was correlated with the meta-analytical finding of Boissier and Moné (2001) and Bin Dajem et al (2008) in their studies on the susceptibility of BALB/C and C57 to S. mansoni infection. Boissier and Moné (2001) observed a significant positive relationship between S. mansoni worm burden and male worms' proportion in four host models: rats, mice, hamsters and monkeys.…”

Section: Discussionsupporting

confidence: 56%

Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice

2011

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The repeated chemotherapy of schistosomiasis has resulted in the emergence of drug-resistant schistosome strains. The development of such resistance has drawn the attention of many authors to alternative drugs. Many medicinal plants were studied to investigate their antischistosomal potency. The present work aimed to evaluate antischistosomal activity of crude aqueous extract of ginger against Schistosoma mansoni. Sixteen mice of C57 strain were exposed to 100 ± 10 cercariae per mouse by the tail immersion method; the mice were divided into two groups: untreated group and ginger-treated one. All mice were sacrificed at the end of 10th week post-infection. Worm recovery and egg counting in the hepatic tissues and faeces were determined. Surface topography of the recovered worms was studied by scanning electron microscopy. Histopathological examination of liver and intestine was done using routine histological procedures. The worm burden and the egg density in liver and faeces of mice treated with ginger were fewer than in non-treated ones. Scanning electron microscopical examination revealed that male worms recovered from mice treated with ginger lost their normal surface architecture, since its surface showed partial loss of tubercles' spines, extensive erosion in inter-tubercle tegumental regions and numerous small blebs around tubercles. Histopathological data indicated a reduction in the number and size of granulomatous inflammatory infiltrations in the liver and intestine of treated mice compared to non-treated mice. The results of the present work suggested that ginger has antischistosomal activities and provided a basis for subsequent experimental and clinical trials.

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Section: Discussionsupporting

confidence: 56%

Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice

2011

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“…Using C57BL/6 strain of mice is in accordance with previous studies which showed that this strain was more susceptible to the infection with Egyptian strain of S. mansoni than BALB/c mice strain (Bin Dajem et al 2008), and is known to respond better to UV-irradiated cercariae (Murrell et al 1979;Richter et al 1993;Mountford et al 2001). In addition, Incani et al (2001) reported that the strain of the host appeared to influence the susceptibility to infection, the fecundity, and the percentage of eggs distributed in liver and in intestine during chronic stage of infection.…”

Section: Discussionsupporting

confidence: 84%

Structural changes of Schistosoma mansoni adult worms recovered from C57BL/6 mice treated with radiation-attenuated vaccine and/or praziquantel against infection

et al. 2011

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Although the current treatment of schistosomiasis relies largely on praziquantel (PZQ), it has not significantly reduced the overall number of disease cases, perhaps due to inevitable resistance to PZQ. Previous studies showed that radiation-attenuated vaccine gives protection levels for Schistosoma mansoni in host various species. In the present study, we evaluated the effect of various vaccination strategies in C57BL/6 mice, including single or multiple vaccination strategy, subcurative dose (20 mg/kg) of PZQ, and a combination of single vaccination with subcurative dose of PZQ. Groups of five mice were sacrificed postinfection in 42 days and schistosomes were collected by perfusion and examined by scanning electron microscopy. Treatment either with subcurative dose of PZQ or with a single vaccination of attenuated cercariae (500 per mouse), caused significant reduction in total worm burden, hepatic and intestinal ova counts 43.03%, 73.2%, 59.5% and 37.97%, 52.02%, 26.3%, respectively. Furthermore, tegumental changes were observed, including severe swelling, fusion of tegumental folds, vesicle formation, and loss or shortening of the spines on the tubercles. However, multiple vaccination strategy resulted in much higher reduction in total worm burden, hepatic and intestinal ova count. However, multiple vaccination strategy resulted in high reduction of worm burden, hepatic and intestinal ova counts 72.5%, 90.7%, 65.79%, respectively, and further causing swollen, disruption of tubercles teguments and erosion, extensive peeling, fusion of tegumental folds. Our findings suggest that multiple vaccination strategy is the most effective strategy to clear schistosomal infection, indicating its potential in guiding the design of appropriate therapeutic strategy against schistosomes.

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“…Previous study showed that C57BL/6 strain of mice was more susceptible to the infection with Egyptian strain of S. mansoni than BALB/c mice strain (Bin Dajem et al 2008) and is known to respond better to UV-irradiated cercariae (Murrell et al 1979;Richter et al 1993;Mountford et al 2001). We intentionally used C57BL/6 mice because they seem to be high responders than BALB/c mice who showed moderate response as reported by Mountford et al (2001).…”

Section: Discussionmentioning

confidence: 83%

Ultrastructural changes of adult Schistosoma mansoni worms recovered from C57BL/6 mice passively immunized with normal and vaccinated rabbit sera in vivo

et al. 2011

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The purpose of the study is to explore the role of humoral immunity against Schistosoma mansoni infection in C57BL/6 mice using highly resistant rabbits that had been exposed to three separate immunizations with ultraviolet (UV)-irradiated cercariae (8,000 attenuated cercariae/rabbit in each immunization), and their sera were tested for their ability to transfer protection against S. mansoni challenge. The present study showed the reduction in challenge worm burden had reached 32.76-43.64% when compared with recipients of normal serum or no serum. The surface topography of the worms collected from immunized mice with either normal rabbit sera (NRS) or vaccinated rabbit sera (VRS) revealed severe tegumental alterations, especially in the VRS group. Worms collected from groups that were immunized by NRS or VRS postinfection (200 normal cercariae/mouse) by day 42. Worms from group immunized with NRS showed damage in the tegument, characterized by severe swelling or erosion of tegumental folds, accompanied by changes in tubercles, swelling, shortening, and loss of spines in male worms. The alteration in female tegument was characterized by swelling of tegumental folds, atrophy of ventral sucker, damage of sensory papillae along all the body, severe peeling in some regions, and appearance of few small blebs. VRS induced more severe tegumental damage than NRS in both male and female worms. Severe shrunken vesicles were protruded from the surface between the two suckers. The tegument of the male showed a collapse of tubercles followed by the appearance of vesicles on their surfaces, fusion, erosion, and superficial focal peeling of tegumental folds. In the female worms, severe damage to the oral sucker, the surface between the two suckers, extensive peeling, severe swelling of the tegument, and damage of sensory papillae. In conclusion, the present study support the hypothesis that high levels of antibodies were developed in rabbit sera after multiple exposures to attenuated cercariae of S. mansoni. Furthermore, immunization might have transferred protection against the infection, indicated by severe morphological alterations, a sign of elimination of the worms. Further investigation is being carried out to reveal the molecular mechanisms underlying the transfer of protection.

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Susceptibility of two strains of mice to the infection with Schistosoma mansoni: Parasitological and biochemical studies

Cited by 19 publications

References 36 publications

Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice

Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice

Structural changes of Schistosoma mansoni adult worms recovered from C57BL/6 mice treated with radiation-attenuated vaccine and/or praziquantel against infection

Ultrastructural changes of adult Schistosoma mansoni worms recovered from C57BL/6 mice passively immunized with normal and vaccinated rabbit sera in vivo

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