Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms

Marmunti, Mónica; Gutiérrez, Ana María; Gavazza, Mariana Beatriz; Williams, Sara Inés; Palacios, Alejandro

doi:10.30972/vet.2311798

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Regarding the effects of ChA on viability after 72 hr of storage, it is well known that excessive intracellular ROS levels induce cell damage often resulting in cell death (Marmunti et al., ; Waterhouse et al., ). In this study, the anti‐ROS effect of ChA was suggested by the improvement in the sperm viability and motility and the percentage of structurally intact acrosomes.…”

Section: Discussionmentioning

confidence: 99%

“…One of the most important causes of reduced semen quality during storage time is the presence of reactive oxygen species (ROS), which in turn cause oxidative damage in sperm cell membranes (Waterhouse et al., ). The presence of ROS is almost always related to the alteration of permeability and cell membrane function, apoptotic‐like modifications in the cells (Marmunti, Gutiérrez, Gavazza, Williams, & Palacios, ), reduction in sperm motility and fertility capacity of the semen (Martin‐Hidalgo et al., ). To reduce the damage caused by ROS, antioxidant substances are commonly added to the semen dilution media, ensuring better quality of the cooled semen (Breininger, Beorlegui, O'Flaherty, & Beconi, ; Martin‐Hidalgo et al., ; Mendez et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Chlorogenic acid improves the quality of boar semen subjected to cooled storage at 15°C

et al. 2018

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The aim of this study was to investigate the effect of chlorogenic acid (ChA) in boar semen stored at 15°C. Twelve ejaculates were processed into insemination doses at different concentrations of ChA (0.0, 1.5, 3.0, 4.0 and 6.0 mg/ml) or vitamin E (200 μl/ml) as positive control. Semen was analysed after 0, 24 and 72 hr of storage. ChA improved (p < .05) sperm motility, acrosome integrity and mitochondrial activity in all periods of storage. Furthermore, after 24 hr of storage, ChA above 1.5 mg/ml supported the sperm viability until 120 min after reheating (p < .05). Both ChA and vitamin E were similarly efficient in increasing the antioxidant capacity of semen, reducing the malondialdehyde levels before and after 72 hr of storage (p < .05). However, with 72 hr of storage, ChA at 3.0 mg/ml improved the mitochondrial activity over vitamin E (p < .05). In conclusion, results suggest that the concentration of 3.2 mg/ml of ChA is the best for semen stored for up to 24 hr. However, for semen stored for a longer period, 6.0 mg/ml or more should be used.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chlorogenic acid improves the quality of boar semen subjected to cooled storage at 15°C

et al. 2018

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“…Excessive ROS can damage the sperm by causing the lipid peroxidation (LPO) and DNA damage. LPO also reduces the level of PUFA (Marmunti et al, 2012). Egg yolk (15% to 20%) has been used for over 40 years as a standard membrane-modifying agent that is added to traditional semen cryopreservation extender (Phillips and Lardy, 1940;Visser and Salamon, 1974).…”

Section: Replacing Of Egg Yolk With Lecithin In Boar Semen Cryopreser...mentioning

confidence: 99%

Single fixed-time artificial insemination with fresh or frozen thawed semen in pig

Pearodwong

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Single fixed-time AI is the breeding protocol inseminating the sows once with either fresh or frozen-thawed (FT) semen at fixed time after ovulation induction without estrus detection. To develop the success single fixed-time AI protocol in Thailand, factors affecting the efficacy of single fixed-time AI were evaluated. Additionally, we also evaluated the effect of using lecithin in cryopreservation extender on FT semen qualities in order to find the possibility to perform single fixed-time AI using sperm preserved in lecithin based extender. It was found that administration of 10 ug buserelin at 72 h after weaning decreased weaning-to-ovulation interval compared to spontaneous ovulation (127.6 vs. 157.2 h). However, the response of buserelin injection was also affected by season and weaned sows conditions. Hot season prolonged ovulation time. Weaned sows with lactation length (LL) ?20 days, litter weight (LW) <67 kg, or body condition score (BCS) ?3, or high backfat reserve after weaning had better buserelin responses. The single fixed-time AI using 10 ug buserelin injection at 72 h after weaning and insemination the sows at 32 h later was successfully performed under tropical climate with acceptable litter size compared to conventional AI (12.0 vs. 12.8 piglets per litter). A decreased litter size was also observed when single fixed-time AI was performed in the hot season or using intrauterine insemination (IUI) with a reduced number of sperm. Finally, the FT semen qualities of sperm preserved in cryopreservation extenders containing 20% egg yolk or 3% lecithin and 10% egg yolk were higher than those in 6% lecithin extender. In conclusion, the present study found that hot season decreased response of buserelin injection, delayed ovulation time and decreased sows reproductive performances after single fixed-time AI. Weaned sows with LL ?20 days, LW <67 kg, BCS ?3 or high backfat had better responses to buserelin injection. Single fixed-time AI using the IUI with a reduced number of sperm decreased litter size. Replacing of egg yolk with lecithin in cryopreservation extender did not increase FT semen qualities. The precise concentration of lecithin should be further investigated to perform single fixed-time AI using FT semen preserved in lecithin-based extender.

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Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

Pereira

Rocha

Teles

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

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Susceptibility to peroxidation and fatty acid composition of fresh boar semen obtained from different hog farms

Cited by 3 publications

References 19 publications

Chlorogenic acid improves the quality of boar semen subjected to cooled storage at 15°C

Chlorogenic acid improves the quality of boar semen subjected to cooled storage at 15°C

Single fixed-time artificial insemination with fresh or frozen thawed semen in pig

Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

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