Suspension cultures of strain L mouse fibroblasts

Danes, B. Shannon

doi:10.1016/0014-4827(57)90305-1

Cited by 18 publications

(3 citation statements)

References 5 publications

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“…Cells were grown in stirrer cultures in a 1 1. aspirator bottle stirred with a Teflon magnet with an inlet and outlet for gassing and an inlet and outlet for feeding and withdrawing the cells (see Danes, 1957;McLimus, Davis, Glover & Rake, 1957). The medium used was Eagle's basal medium (BME) plus glutamine (1.6 mm), and calf serum 5 %, obtained from Flow Laboratories.…”

Section: Culture Methodsmentioning

confidence: 99%

Effect of ouabain and metabolic inhibitors on the Na and K movements of nucleotide contents of L cells

Lamb¹,

Mackinnon²

1971

The Journal of Physiology

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SUMMARY1. The general characteristics and Na and K movements of L cells (derived from mouse epithelium) have been measured. Both cells grown in suspension (LS cells) and as a monolayer (L cells) were used.2. The volume of L cells was 1-2 x 10-9 cm3 and of LS cells 3-5 x 10-9 cm3; of this 82 % was water.3. Electron micrographs showed the presence of numerous protrusions (filopodia) from both forms of the cell. These had the effect of increasing the surface area of the cell by 2-4 times over smooth cells of the same volume. On changing from the flattened to the spherical shape during trypsinization, the filopodia altered to maintain a constant V/A ratio. 6. Both DNP (10-3M) and IAA (IO 4M) are required for maximum inhibition of K uptake, as both aerobic and anaerobic metabolic pathways may be used to drive the pump.7. K removal decreases the Na efflux, and Na removal (eventually) decreases the K influx providing evidence for Na/K coupling.

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Section: Culture Methodsmentioning

confidence: 99%

Effect of ouabain and metabolic inhibitors on the Na and K movements of nucleotide contents of L cells

Lamb¹,

Mackinnon²

1971

The Journal of Physiology

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“…These include the tumble tube of Owens el aZ. (5), and a variety of modifications of the foregoing ( 6,7,8,9,10). (2), the roller tube of Graham and Siminovitch ( 3 ) .…”

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confidence: 99%

Effect of Pluronic F68 on Growth of Fibroblasts in Suspension on Rotary Shaker

Swim¹,

Parker²

1960

Experimental Biology and Medicine

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“…Once stable and functionally defined growth mediums are developed from defined mediums now under study (101, 102, 103, 235, 256, 343, 39 1), and genetic control of cells in culture is achieved by routinely applicable procedures, it may be anticipated that for many purposes continuous sus pension culture (42,52,67,73,91,234,247, 319, 374, 41 1) will be employed as a basis of cultural characterization. The technique is desirable for study of physiologically variable aspects of cell character because these should be assessed under steady-state conditions.…”

Section: Introductionmentioning

confidence: 99%