Background:The corona virus disease 2019 (COVID-19) has caused devastating effects by spreading rapidly throughout society worldwide since it was identified at the end of December 2019 in Wuhan City, China. Aim of the study: was to assess effect of COVID-19 pandemic on knowledge and anxiety level Among nursing internship students. Subjects and Methods: Research design: A descriptive design was used to conduct this study and to achieve the aim of this study. Setting: The study was carried out at Technical Institute of Nursing in Zagazig University. Subjects: A convenience sample of all available internship students (618 students) at the technical institute of nursing in Zagazig University. Tools of data collection: Two tools were used for data collection. Tool I: Structured Interviewing Questionnaire, It was composed of 2 parts, General Characteristics of the nursing internship students, Self-administered Knowledge questionnaire. Tool II: Hamilton Anxiety scale. Results: 37.9% of the studied subjects had fair knowledge regarding COVID -19. In addition, 54.9% of the studied subjects had moderate anxiety level. On the other hand, knowledge score was inversely correlated with the Hamilton anxiety score (r=-0.122, p=0.005) .Conclusion: More than one third of studied subjects had fair knowledge regarding COVID -19. As well, more than half of them had moderate anxiety level. Recommendations: Continuous campaigns are required for nurses to prevent and control COVID-19 successfully with illustrated booklets for maintaining knowledge, emphasis should also be placed on training and retraining health professionals in the correct hand washing steps as well as media awareness campaigns on this disease.