Sustainable Approach for Peroxygenase-Catalyzed Oxidation Reactions Using Hydrogen Peroxide Generated from Spent Coffee Grounds and Tea Leaf Residues

Abstract

Peroxygenases are promising catalysts for use in the oxidation of chemicals as they catalyze the direct oxidation of a variety of compounds under ambient conditions using hydrogen peroxide (H 2 O 2 ) as an oxidant. Although the use of peroxygenases provides a simple method for oxidation of chemicals, the anthraquinone process currently used to produce H 2 O 2 requires significant energy input and generates considerable waste, which negatively affects process sustainability and production costs. Thus, generating H 2 O 2 for peroxygenases on site using an environmentally benign method would be advantageous. Here, we utilized spent coffee grounds (SCGs) and tea leaf residues (TLRs) for the production of H 2 O 2 . These waste biomass products reacted with molecular oxygen and effectively generated H 2 O 2 in sodium phosphate buffer. The resulting H 2 O 2 was utilized by the bacterial P450 peroxygenase, CYP152A1. Both SCG-derived and TLR-derived H 2 O 2 promoted the CYP152A1-catalyzed oxidation of 4-methoxy-1-naphthol to Russig’s blue as a model reaction. In addition, when CYP152A1 was incubated with styrene, the SCG and TLR solutions enabled the synthesis of styrene oxide and phenylacetaldehyde. This new approach using waste biomass provides a simple, cost-effective, and sustainable oxidation method that should be readily applicable to other peroxygenases for the synthesis of a variety of valuable chemicals.

Isolation and characterization of filamentous fungi capable of degrading the mycotoxin patulin

“…Reaction products were analyzed by HPLC using an LC‐20 system (Shimadzu, Kyoto, Japan) equipped with a Cosmosil 5C18‐PAQ packed column (4.6 × 250 mm, Nacalai Tesque, Kyoto, Japan) (Kawana et al, 2022; Oya et al, 2022). The post‐reaction mixture (200 μL) was acidified by the addition of HCl (pH 2–3), and methanol (300 μL) was then added.…”

Electrochemical transformations catalyzed by cytochrome P450s and peroxidases

“…318 In another approach, H 2 O 2 also drives the epoxidation of styrene to styrene epoxide catalyzed by Cyt P450s, although selectivity is much lower. 325 However, most alkenes are very hydrophobic with a limited solubility in aqueous reaction media. At a low concentration of substrates, Cyt P450s also reduce oxygen through two-electron pathways to produce H 2 O 2 that can oxidize alkenes directly or activate the Cyt P450 via the peroxide shunt (Scheme 2) to epoxide or aldehyde as a mixture.…”

“…At a low concentration of substrates, Cyt P450s also reduce oxygen through two-electron pathways to produce H 2 O 2 that can oxidize alkenes directly or activate the Cyt P450 via the peroxide shunt (Scheme 2) to epoxide or aldehyde as a mixture. 309,325,326…”

“…At a low concentration of substrates, Cyt P450s also reduce oxygen through two-electron pathways to produce H 2 O 2 that can oxidize alkenes directly or activate the Cyt P450 via the peroxide shunt (Scheme 2) to epoxide or aldehyde as a mixture. 309,325,326 In our group, the LBL films of pure CYP1A2, and bacterial Cyt P450cam with conductive polymer PSS on a carbon cloth electrode were used for electrochemical oxidation of styrene to styrene oxide. 226 The epoxidation was facilitated at À0.6 V vs. SCE by initial biocatalytic reduction of molecular oxygen to H 2 O 2 which activates iron heme enzymes to PFe IV QO by a complex mechanism (Fig.…”

Enzyme Activity and Organic Carbon Content in Agricultural Spodosol

“…ПФО участвуют в биосинтезе гумусовых кислот за счет катализа реакций окислительной полимеризации ароматических соединений в присутствии кислорода (8). Пероксидазы -ферменты, катализирующие окисление полифенолов и некоторых ароматических аминов с участием кислорода перекиси водорода (9,10). Активность ПФО и ПО может служить индикаторам интенсивности процессов гумусообразования (11) и в основном коррелирует с абиотическими факторами, в меньшей степени на нее влияет видовой состав и биомасса почвенных грибов (12).…”