Sustainable PHA production in integrated lignocellulose biorefineries

Dietrich, Karolin; Dumont, Marie‐Josée; Rio, Luis F. Del; Orsat, Valérie

doi:10.1016/j.nbt.2018.11.004

Cited by 91 publications

(58 citation statements)

References 63 publications

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“…PHA are very promising materials; however, they are disadvantaged in competition with petrochemical polymers by their high production costs [10]. There are several strategies which could facilitate sustainable and economically feasible production of PHA such as utilization of cheap carbon substrates, for instance waste or side products of food industry or agriculture [11], or the use of genetically engineered strains revealing high productivity and substrate-to-product conversion yields [12]. Furthermore, considering all the positive aspects of employing thermophiles in industrial biotechnology mentioned above, also the use of thermophilic PHA producers holds promise to gain sustainable and economically reasonable processes for PHA production.…”

Section: Introductionmentioning

confidence: 99%

Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

et al. 2020

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Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolate Aneurinibacillus sp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 °C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated that Aneurinibacillus sp. H1 is a very promising candidate for production of PHA with tailored material properties.

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Section: Introductionmentioning

confidence: 99%

Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

et al. 2020

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“…Several bacterial strains, for example, Bacillus subtilis, Cupriavidus necator, Haloferax mediterranei, Haloferax halophila, Pseudomonas aeruginosa, and Pseudomonas putida have the ability to produce different types of PHA from a variety of feedstocks (Dietrich et al, 2018;Li and Wilkins, 2020). Even though some reports presented that the utilization of lignocellulosic feedstocks is challenging owing to its low fermentability, the utilization of agroindustrial wastes can be an attractive option to produce PHA using xylose-rich hydrolysates (Dietrich et al, 2018). However, inhibitors (furfural, hydroxymethylfurfural, and lignin derivatives) present in the lignocellulosic hydrolysates might affect the capability of fermenting microorganisms and might reduce the productivity (Li and Wilkins, 2020).…”

Section: Potential Bioproducts For Biorefineries Biopolymersmentioning

confidence: 99%

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

et al. 2020

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The integrated approach in biorefinery mainly involves the utilization of various agroindustrial byproducts such as raw materials for the production of several biobased products like biofuels, bioenergy, and other high-value chemicals. Biofuels are the backbone of biorefineries, however, production of value-added biomolecules such as biopigments, biopolymers, biosurfactants, and nutritional yeast has been attracting great attention. The production of these biomolecules using traditional approaches has been extensively studied in the last few years owing to their promising application in different industries such as chemical, food/feed, and pharmaceuticals for the development of novel products for mankind. Moreover, the production of such biomolecules using lignocellulosic, starchy, and some other agroindustrial byproducts is still not fully explored. Hence, there is a huge scope in the development of sustainable biorefining approaches to make the technology cost-effective. The lignocellulosic biomasses usually used in biorefineries are mainly composed of cellulose, hemicellulose, and lignin, whereas starchy materials, besides starch, usually contain, protein, lipids, and some micronutrients. The processing of these biomasses through successive steps like pretreatments, enzymatic hydrolysis, and fermentation is essentially required to obtained final biobased products. Considering certain bottlenecks of above-mentioned conventional biorefineries approaches, new technologies have been proposed for the improved pretreatment of biomass and efficient enzymatic hydrolysis in order to minimize the concentration of toxic inhibitors in resulting hydrolysate. In this review, we highlighted the different agroindustrial byproducts and their applications for the production of valuable biorefinery products.

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“…In this way, waste streams can be redirected into new production processes to close the loops on valuable organic resources, the major principle of a circular bioeconomy. The idea of integrated bio-refineries for the synthesis of biopolymers has attracted the attention of industry and academia likewise: using organic waste materials as a sustainable carbon and energy source for microorganisms able to produce the biomolecules of interest [11].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of poly-3-hydroxybutyrate (PHB) by Bacillus cereus using grape residues as sole carbon source

Andler

Pino

Moya

et al. 2021

International Journal of Biobased Plastics

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Polyhydroxyalkanoates (PHA) are bacterial polyesters that have been described as one of the most promising sustainable alternatives to petroleum-based plastics. A major challenge for PHA to become market-competitive, is to decrease the associated production costs. According to several studies, about 20-50% of the total production costs is attributed to the raw materials and therefore, the use of different types of organic residues have been evaluated as substrates for the microorganisms. In this study, the strain Bacillus cereus ATCC 14579 was cultivated in shake flasks using a defined medium and pretreated grape peel as sole carbon source. The pretreatment of the grape peels was performed by a hydrolysis step with diluted sulfuric acid yielding up to 52.9% (w/w) of total sugars from the dry residue. PHB extraction using dry and wet biomass was performed and evaluated showing up to 3.7-higher yields when using the latter. PHB accumulation of 18.79% (w/w), corresponding to 0.53 g PHB /L, was achieved using the grape peel hydrolyzate at 72 h of cultivation. Eventually, the possibility of producing PHB by the use of non-treated lignocellulosic biomass (LCB) with high contents of fermentable sugars is presented as an interesting alternative to be optimized and projected for sustainable bioplastic production processes.

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Sustainable PHA production in integrated lignocellulose biorefineries

Cited by 91 publications

References 63 publications

Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

Synthesis of poly-3-hydroxybutyrate (PHB) by Bacillus cereus using grape residues as sole carbon source

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