This paper analyzes the impact of C-ITS service on driving behavior, focusing on a pilot project in Daejeon–Sejong, South Korea. C-ITS, an advanced technology, enables bidirectional wireless communication between vehicles or infrastructure, allowing for real-time traffic data collection and dissemination. The study uses a unique analytical method, employing parallel processing techniques for variable extraction and a paired t-test to examine the short-term effects of C-ITS on driving behavior. Findings indicate a significant change in drivers’ behavior, particularly in average speed, hard braking rate, severe deceleration rate, speeding rate, and excessive speeding rate, towards safer trends after receiving C-ITS warning services. Reductions in hard braking and severe deceleration were immediate after C-ITS service initiation, while a decrease in excessive speeding was observed after four months. Further research is needed to identify other potential influencing variables and provide an unbiased evaluation of C-ITS effectiveness. The study’s implications highlight its role in promoting public acceptance of C-ITS-service-based cooperative autonomous driving strategies.