Sustained Immobilization of Growth Factor Proteins Based on Functionalized Parylenes

Chen, Yung-Chih; Sun, Ting-Pi; Su, Chiao-Tzu; Wu, Jyun‐Ting; Lin, Chih-Yeh; Yu, Jihong; Huang, Chao‐Wei; Chen, Chia-Jie; Chen, Hsien‐Yeh

doi:10.1021/am5071865

Cited by 29 publications

(24 citation statements)

References 32 publications

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“…The syntheses of the two dimers were conducted following previously reported procedures. 35 , 40 During the CVD process, a dual-sourced configuration for the CVD system was used for the copolymerization. Specifically, the feeding ratio (molar) of 4- N -hydroxysuccinimide ester-[2,2]-paracyclophane and 4-methyl-propiolate-[2,2]paracyclophane was controlled at 1:1, and pyrolysis temperatures of 700 and 650 °C were used to transform the two dimers into the corresponding quinodimethanes, respectively.…”

Section: Methodsmentioning

confidence: 99%

Vapor sublimation and deposition to build porous particles and composites

et al. 2018

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The vapor deposition of polymers on regular stationary substrates is widely known to form uniform thin films. Here we report porous polymer particles with sizes controllable down to the nanometer scale can be produced using a fabrication process based on chemical vapor deposition (CVD) on a dynamic substrate, i.e., sublimating ice particles. The results indicate that the vapor deposition of a polymer is directed by the sublimation process; instead of forming a thin film polymer, the deposited polymers replicated the size and shape of the ice particle. Defined size and porosity of the polymer particles are controllable with respect to varying the processing time. Extendable applications are shown to install multiple functional sites on the particles in one step and to localize metals/oxides forming composite particles. In addition, one fabrication cycle requires approximately 60 min to complete, and potential scaling up the production of the porous particles is manageable.

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Section: Methodsmentioning

confidence: 99%

Vapor sublimation and deposition to build porous particles and composites

et al. 2018

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“…Another example demonstrated the use of parylene coatings in the sustainable immobilization of GFs via a maleimide-thiol coupling reaction under mild conditions. 40 The site-specific approach defined in this study produced preferential bonds without damaging the tertiary structure of the GFs or compromising their biological activities.…”

Section: Covalent Conjunctionsmentioning

confidence: 98%

Novel biomaterial strategies for controlled growth factor delivery for biomedical applications

et al. 2017

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Growth factors (GFs) are soluble proteins secreted by cells that have the ability to regulate a variety of cellular processes and tissue regeneration. However, their translation into clinical applications is limited due to their short effective half-life, low stability, and rapid inactivation by enzymes under physiological conditions. To maximize the effectiveness of GFs and their biologically relevant applicability, a wide variety of sophisticated bio-inspired systems have been developed that augment tissue repair and cellular regeneration by controlling how much, when, and where GFs are released. Recently, protein immobilization techniques combined with nanomaterial carriers have shown promise in mimicking the natural healing cascade during tissue regeneration by augmenting the delivery and effectiveness of GFs. This review evaluates the latest techniques in direct immobilization and relevant biomaterials used for GF loading and release, including synthetic polymers, albumin, polysaccharides, lipids, mesoporous silica-based nanoparticles (NPs), and polymeric capsules. Specifically, we focus on GF-encapsulated NPs in functionalized microporous scaffolds as a promising alternative with the ability to mimic extracellular matrix (ECM) hierarchical architectures and components with high cell affinity and bioactivity. Finally, we discuss how these next-generation, advanced delivery systems have been used to enhance tissue repair and regeneration and consider future implications for their use in the field of regenerative medicine.

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“…[8,5,9] The presence of substituents such as aldehyde, ketone, alcohol, ester,a mine, as well as fluorinated groups, thiol reactive groups,A TRP initiator groups, photosensitiveo ra lkynyl groups in the precursor and consequently in the polymer coatingh as enabled variousp ost-modification strategies. [2,[10][11][12][13][14][15][16][17][18][19][20][21][22][23] Attachmento fp roteins, short peptides, nucleotides, and other smallb iologically activem olecules onto this functional coating can dramatically influence the response of adherent cells. [10,11,13,19] Therefore, precise design of surface chemistry provided by CVD enables the control of cell behavior by tailoringi nteractions between CVD coatings and living organisms.…”

Section: Introductionmentioning

confidence: 99%

“…[2,[10][11][12][13][14][15][16][17][18][19][20][21][22][23] Attachmento fp roteins, short peptides, nucleotides, and other smallb iologically activem olecules onto this functional coating can dramatically influence the response of adherent cells. [10,11,13,19] Therefore, precise design of surface chemistry provided by CVD enables the control of cell behavior by tailoringi nteractions between CVD coatings and living organisms. [24] However, strategies taking advantage of the Gorhamp rocess to deposit vapor-based reactive coatings have so far almosta lwaysb een limitedt op oly-p-xylylenes, that is, entirelyc arbon-based polymer backbones with prominent hydrophobicity.…”

Section: Introductionmentioning

confidence: 99%

Polylutidines: Multifunctional Surfaces through Vapor‐Based Polymerization of Substituted Pyridinophanes

Gall

Hussal

Kramer

et al. 2017

Chemistry A European J

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We report a new class of functionalized polylutidine polymers that are prepared by chemical vapor deposition polymerization of substituted [2](1,4)benzeno[2](2,5)pyridinophanes. To prepare sufficient amounts of monomer for CVD polymerization, a new synthesis route for ethynylpyridinophane has been developed in three steps with an overall yield of 59 %. Subsequent CVD polymerization yielded well-defined films of poly(2,5-lutidinylene-co-p-xylylene) and poly(4-ethynyl-2,5-lutidinylene-co-p-xylylene). All polymers were characterized by infrared reflection-absorption spectroscopy, ellipsometry, contact angle studies, and X-ray photoelectron spectroscopy. Moreover, ζ-potential measurements revealed that polylutidine films have higher isoelectric points than the corresponding poly-xylylene surfaces owing to the nitrogen atoms in the polymer backbone. The availability of reactive alkyne groups on the surface of poly(4-ethynyl-2,5-lutidinylene-co-p-xylylene) coatings was confirmed by spatially controlled surface modification by means of Huisgen 1,3-dipolar cycloaddition. Compared to the more hydrophobic poly-p-xylylyenes, the presence of the heteroatom in the polymer backbone of polylutidine polymers resulted in surfaces that supported an increased adhesion of primary human umbilical vein endothelial cells (HUVECs). Vapor-based polylutidine coatings are a new class of polymers that feature increased hydrophilicity and increased cell adhesion without limiting the flexibility in selecting appropriate functional side groups.

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Sustained Immobilization of Growth Factor Proteins Based on Functionalized Parylenes

Cited by 29 publications

References 32 publications

Vapor sublimation and deposition to build porous particles and composites

Vapor sublimation and deposition to build porous particles and composites

Novel biomaterial strategies for controlled growth factor delivery for biomedical applications

Polylutidines: Multifunctional Surfaces through Vapor‐Based Polymerization of Substituted Pyridinophanes

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