Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study

Abstract

Enterococcus faecalis is the most commonly recovered species from failed root canal treatments. In this study, we tested the capability of a novel intracanal sustained-release filler (SRF) containing cetylpyridinium chloride (CPC) to disinfect dentinal tubules of segmented human tooth specimens. Human dental root specimens were infected with E. faecalis V583 for 3 weeks in a static environment. The tested intracanal medicaments were SRF-CPC and calcium hydroxide (CH). Each medicament was introduced into the canal of the dental specimen and incubated for 7 days. The bacteriological samples were taken by shaving the dentine surrounding the root canal with dental burs ranging in size from ISO 014-020. The obtained dentine powder was collected in test tubes containing phosphate-buffered saline, sonicated, and plated on agar plates. Colony-forming units were counted after 48 h of incubation. Random specimens were also examined under confocal laser scanning microscopy and scanning electron microscopy. A statistical difference was found in the bacterial counts obtained from all layers of infected dentin between the control and the SRF-CPC groups. CH reduced bacterial viability significantly only in the first layer of the infected dentin, up to 150 μm into the dentinal tubules. CLSM images showed that SRF-CPC killed most bacteria throughout the infected dentin up to 700 μm of penetration. SEM images demonstrated the adhesion ability of SRF-CPC to the dentinal wall. In conclusion, SRF-CPC is a potential intracanal medicament for disinfecting dentinal tubules.

One-Pot Preparation of Cetylpyridinium Chloride-Containing Nanoparticles for Biofilm Eradication

“…26 CPC-containing sustained-release filler enables an effective release of the drug that can be used in root canal treatment. 27,28 Previous studies attempted several approaches to employ CPC as a template to synthesize MSNs under various conditions. 11,29−31 Yet, the requirements may involve other organic solvents and mixed surfactants, resulting in relatively lengthy synthetic procedures limiting the further biological applications.…”

“… 26 CPC-containing sustained-release filler enables an effective release of the drug that can be used in root canal treatment. 27 , 28 …”

“…Recently, a hybrid CPC–montmorillonite composite material was successfully tested to prevent dental caries through its gradual release and rechargeability . CPC-containing sustained-release filler enables an effective release of the drug that can be used in root canal treatment. , …”

Corrigendum to “Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study”

“…In the article titled “Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study” [ 1 ], the corresponding author's e-mail address was given incorrectly. The correct e-mail address is bernhard.funk@mail.huji.ac.il , as shown above.…”