Sustainment of Antioxidant Enzymes by Drosera peltata in Ehrlich's Ascites Carcinoma (EAC) Bearing Mice

Abstract

The purpose of this study was to measure the antioxidant status of animals treated with 250 and 500 mg / kg doses of ethanol and aqueous extract of Drosera peltata on Erich ascites carcinoma (EAC) inoculated mice. A total of 70 mice were divided into 7 groups, each group with ten mice. The first group received normal food and water for 14 days and was under normal control. The second group also received normal food and water for 14 days, which was cancer control. Third group received 5- flurouracil (20 mg/kg, i.p) for 14 days. Fourth and fifth group animals received 250 and 500 mg / kg of ethanol extracts of D. peltata (EEDP) whereas the sixth and seventh groups of mice received 250 and 500 mg / kg of aqueous extracts of D. peltata (AEDP), orally for 14 days. All the groups were inoculated with EAC (2×106 cells/mouse, i.p.) except Group I, 24 hours before the commencement of the drug treatment. After the completion of treatment, blood was drawn retro-orbitally and sacrificed to isolate the liver, lungs, kidneys, and brain for observing tissue antioxidant status. The parameters analyzed were total protein (TP), catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (P), and glutathione (GSH) from the tissues apart and the protein carbonyl content (PCC) also measured from the blood sample. Treatment with EEDP and AEDP significantly lowers the MDA levels from 23 to 10 mmol/ml in the blood whereas 28 to 4 nm/g tissue isolates the liver, lungs, kidneys, and brain. It also raised the TP, GSH, SOD, CAT, and P levels in the blood and the tissues sample of the cancer cell line inoculated animals, and their levels are maintained similarly to normal group animals. The results proposed that both the extracts of D. peltata retained the various tissue antioxidant statuses in mice with EAC cancer lines.