Süt ve Süt Ürünlerinde Listeria monocytogenes Varlığının Belirlenmesi, Antibiyotik Duyarlılık Profillerinin Saptanması, Izolatların Serolojik ve Moleküler Yöntemlerle Tiplendirilmesi

Dümen, Emek; Issa, Ghassan; İkiz, Serkan; Bağcıgil, Arzu Funda; Ozgur, Yakut; Kahraman, Tolga; Erǧin, Sevgi; Yeşil, Osman

doi:10.9775/kvfd.2010.3632

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The typical colonies were identified by biochemicals tests and confirmed with Salmonella antiserum (O and H-Vi polyvalent antisera) [13] . [14,15] .…”

Section: Bacteriological Analysismentioning

confidence: 99%

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

İkiz¹,

Dümen²,

Kahraman³

et al. 2016

Kafkas Univ Vet Fak Derg

Self Cite

View full text Add to dashboard Cite

The present study was conducted to investigate the presence of Salmonella spp. and Listeria monocytogenes in 700 seafood (400 raw fish, 100 raw shrimps and 200 raw molluscs) collected from retailers. Isolations were performed by conventional culture methods. The isolates were also confirmed by PCR assays. Salmonella spp. and L. monocytogenes were detected in 9.9% and 3.86% fish and shellfish samples, respectively. The highest rates of Salmonella spp. (12.5%) were found in fish samples and L. monocytogenes (7.0%) were in shrimp samples. Therefore, it is essential to ensure improving the quality of production technology from fishing to retail outlet and developing the sanitation conditions of food contact surfaces and handling areas. Keywords

show abstract

“…The typical colonies were identified by biochemicals tests and confirmed with Salmonella antiserum (O and H-Vi polyvalent antisera) [13] . [14,15] .…”

Section: Bacteriological Analysismentioning

confidence: 99%

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

İkiz¹,

Dümen²,

Kahraman³

et al. 2016

Kafkas Univ Vet Fak Derg

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many variables including the number and rate of saprophytic microorganisms available in the environment, geographic regions and infected food affect genetic variety and the resistance of L. monocytogenes serotypes to antibiotics which in turn lead to different research results [44] . The present study also involves results which are different from those of some other researchers.…”

Section: Discussionmentioning

confidence: 99%

Çiğ Süt ve Süt Ürünlerinde Listeria monocytogenes Varlığı ve Antibiyotik Direnci

Aksoy¹,

Sezer²,

Vatansever³

et al. 2018

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

How to Cite This ArticleAksoy A, Sezer Ç, Vatansever L, Gülbaz G: Presence and antibiotic resistance of Listeria monocytogenes in raw milk and dairy products . Kafkas Univ Vet Fak Derg, 24 (3): 415-421, 2018. AbstractIn this study, the presence of Listeria monocytogenes in raw milk and milk products produced from raw milk by traditional method, such as cheese and butter using cultural and molecular techniques and the antibiotic resistances were investigated. Isolation was performed using the FDA technique. Microbact 12L test kit was used for biochemical identification. Genotyping of isolates was done by traditional PCR method. Antibiotic resistance of isolates was determined by standard disk diffusion method. Listeria spp. was detected in 16 (5.3%) of the total 300 food samples according to the biochemical identification results. The highest prevalence of Listeria spp. was in raw milk samples (10%), followed by cheese samples (4%) and butter samples (2%). All 36 Listeria spp. isolates which were obtained from biochemical identification were tested for L. monocytogenes by PCR, and 15 (41.6%) of them were confirmed as L. monocytogenes. According to the antibiotic resistance test results of L. monocytogenes strains, four (26.7%) isolates were resistant to at least one antibiotic while only one (6.7%) had multiple antibiotic resistance. The highest resistance was found against trimethoprim-sulfamethoxazole. It has been determined that L. monocytogenes isolates had the same degree resistance against penicillin G, meropenem, amikacin and vancomycin. In conclusion, the presence of L. monocytogenes which has been detected in dairy products made from raw milk and in raw milk itself may constitute a potential risk for public health. Çiğ Süt ve Süt Ürünlerinde Listeria monocytogenes Varlığı ve Antibiyotik DirenciÖz Bu çalışmada, çiğ süt ve geleneksel yöntemlerle çiğ sütten üretilen süt ürünlerinde (peynir ve tereyağ) kültürel ve moleküler teknikler kullanılarak Listeria monocytogenes varlığı ve antibiyotik direnci araştırıldı. İzolasyon FDA tekniğine göre yapıldı. Biyokimyasal identifikasyon için Microbact 12L test kiti kullanıldı. İzolatların genetik olarak tanımlanması klasik PCR yöntemi ile yapıldı. İzolatların antibiyotik direnci standart disk difüzyon yöntemi ile belirlendi. Biyokimyasal identifikasyon sonuçlarına göre toplamda 300 gıda örneğinin 16'sında (5.3%) Listeria spp. tespit edildi. Listeria spp. en yüksek çiğ süt örneklerinde bulunurken (%10), bunu peynir örnekleri (%4) ve (%2) tereyağı örnekleri izledi. Biyokimyasal test sonuçlarına göre elde edilen 36 adet Listeria spp. izolatının tümü L monocytogenes yönünden PCR ile test edildi ve 15'i (%41.6) L. monocytogenes olarak doğrulandı. L monocytogenes izolatlarının antibiyotik direnç test sonuçlarında izolatların dördü (%26.7) en az bir antibiyotiğe dirençli iken biri (%6.7) çoklu antibiyotik dirence sahipti. En yüksek direnç trimetoprim-sülfametoksazole karşı bulundu. L. monocytogenes izolatlarının penisilin G, meropenem, amikasin ve vankomisine karşı aynı ora...

show abstract

Süt ve Süt Ürünlerinde Listeria monocytogenes Varlığının Belirlenmesi, Antibiyotik Duyarlılık Profillerinin Saptanması, Izolatların Serolojik ve Moleküler Yöntemlerle Tiplendirilmesi

Cited by 2 publications

References 5 publications

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

Çiğ Süt ve Süt Ürünlerinde Listeria monocytogenes Varlığı ve Antibiyotik Direnci

Contact Info

Product

Resources

About