SUTAF, a novel β-methoxyacrylate derivative, promotes neurite outgrowth with extracellular signal-regulated kinase and c-jun N-terminal kinase activation

Nagahara, Yukitoshi; Suzuki, Eiji; Sekine, Yuriko; Uchiro, Hiromi; Yoshimi, Yoji; Shinomiya, Takashi; Ikekita, Masahiko

doi:10.1016/j.ejphar.2012.08.018

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…Over the past 2 decades, numerous studies have revealed the molecular mechanisms underlying the regulation of neurite outgrowth by various factors. The representative pathway for neurite outgrowth is the activation of the PI3K/Akt and/or protein kinase A (PKA)/mitogen-activated protein kinase (MAPK)/ERK signaling pathways [16,17,18,19,20,21]. We thus tested by immunoblot analysis whether compound 13 had the ability to promote the phosphorylation of ERK1/2 and/or Akt in neuro2a cells.…”

Section: Resultsmentioning

confidence: 99%

“…These results suggest that compound 13 -induced neurite outgrowth from neuro2a cells was being regulated through the PI3K/Akt-mediated signaling pathway and not the PKA/MAPK/ERK one. Other various signal pathways such as c-jun N-terminal kinase (JNK) have been reported to be involved in neurite outgrowth of neuro2a cells [19,21]. We will investigate whether not only PI3K/Akt-mediated signaling pathway, but also other signaling pathway(s) underlie the compound 13 -induced neuronal differentiation or not.…”

Section: Resultsmentioning

confidence: 99%

“…As a result, we successfully found that one of our carbazole derivatives had this ability as well as anti-oxidant ability; and so, we then investigated the regulatory mechanisms of the neurite outgrowth triggered by this compound. Regarding the regulatory mechanisms at play in neurite outgrowth from neuronal cells (including not only neuro2a cells but also rat PC12 cells), various signal pathways have been reported to be involved, such as extracellular signal-regulated kinase (ERK) [17,18,19], Akt and phosphatidylinositol 3-kinase (PI3K) [16,20]. Thus, we investigated whether our carbazole compound had the ability to directly activate (phosphorylate) any signal transduction molecule(s) and whether the blockage of such signal transduction(s) would reduce this carbazole compound-induced neurite outgrowth from neuro2a cells.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Carbazole Derivatives on Neurite Outgrowth and Hydrogen Peroxide-Induced Cytotoxicity in Neuro2a Cells

et al. 2019

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Many studies have demonstrated that oxidative stress plays an important role in several ailments including neurodegenerative diseases and cerebral ischemic injury. Previously we synthesized some carbazole compounds that have anti-oxidant ability in vitro. In this present study, we found that one of these 22 carbazole compounds, compound 13 (3-ethoxy-1-hydroxy-8- methoxy-2-methylcarbazole-5-carbaldehyde), had the ability to protect neuro2a cells from hydrogen peroxide-induced cell death. It is well known that neurite loss is one of the cardinal features of neuronal injury. Our present study revealed that compound 13 had the ability to induce neurite outgrowth through the PI3K/Akt signaling pathway in neuro2a cells. These findings suggest that compound 13 might exert a neurotrophic effect and thus be a useful therapy for the treatment of brain injury.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Carbazole Derivatives on Neurite Outgrowth and Hydrogen Peroxide-Induced Cytotoxicity in Neuro2a Cells

et al. 2019

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Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways

Tao

Feng

et al. 2016

Cell Mol Neurobiol

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Minocycline, a semi-synthetic second-generation derivative of tetracycline, has been reported to exert neuroprotective effects both in animal models and in clinic trials of neurological diseases. In the present study, we first investigated the protective effects of minocycline on oxygen-glucose deprivation and reoxygenation-induced impairment of neurite outgrowth and its potential mechanism in the neuronal cell line, PC12 cells. We found that minocycline significantly increased cell viability, promoted neurite outgrowth and enhanced the expression of growth-associated protein-43 (GAP-43) in PC12 cells exposed to oxygen-glucose deprivation/reoxygenation injury. In addition, immunoblots revealed that minocycline reversed the overexpression of phosphorylated myosin light chain (MLC) and the suppression of activated extracellular signal-regulated kinase 1/2 (ERK1/2) caused by oxygen-glucose deprivation/reoxygenation injury. Moreover, the minocycline-induced neurite outgrowth was significantly blocked by Calyculin A (1 nM), an inhibitor of myosin light chain phosphatase (MLCP), but not by an ERK1/2 inhibitor (U0126; 10 μM). These findings suggested that minocycline activated the MLCP/MLC signaling pathway in PC12 cells after oxygen-glucose deprivation/reoxygenation injury, which resulted in the promotion of neurite outgrowth.

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L-NBP, a multiple growth factor activator, attenuates ischemic neuronal impairments possibly through promoting neuritogenesis

Zhao

Jiang

et al. 2019

Neurochemistry International

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SUTAF, a novel β-methoxyacrylate derivative, promotes neurite outgrowth with extracellular signal-regulated kinase and c-jun N-terminal kinase activation

Cited by 3 publications

References 29 publications

Effects of Carbazole Derivatives on Neurite Outgrowth and Hydrogen Peroxide-Induced Cytotoxicity in Neuro2a Cells

Effects of Carbazole Derivatives on Neurite Outgrowth and Hydrogen Peroxide-Induced Cytotoxicity in Neuro2a Cells

Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways

L-NBP, a multiple growth factor activator, attenuates ischemic neuronal impairments possibly through promoting neuritogenesis

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