SWATH-MS-based proteomics reveals functional biomarkers of Th1/Th2 responses of tropomyosin allergy in mouse models

Xu, Li; Zhang, Hong Wei; Lin, Hong; Zhang, Xiao Mei; Wen, Yunqi; Zhao, Jinlong; Li, Zhen Xing; Gasset, Marı́a

doi:10.1016/j.foodchem.2022.132474

Cited by 7 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteomics is sufficiently advanced, and integrated with other important omics such as metabolomics and microbiomics, it enables in-depth analysis of many biological processes, which is conducive to enhancing the understanding of the underlying biological mechanism of FA. Xu et al investigated the proteomic changes in the spleen and intestine in mice sensitized with tropomyosin (TM) isolated from shrimp, clams, and fish . TM-induced T-cell responses in the spleen were marked by the upregulation of immune response regulators (cochlin and Ig κ chain V–III region PC 7175), while impairment of metabolic routes, absorption processes, and ammonium ion responses dominated proteomic features in the intestine.…”

Section: Introductionmentioning

confidence: 99%

“…Xu et al investigated the proteomic changes in the spleen and intestine in mice sensitized with tropomyosin (TM) isolated from shrimp, clams, and fish. 11 TM-induced T-cell responses in the spleen were marked by the upregulation of immune response regulators (cochlin and Ig κ chain V−III region PC Student's t test was used to compare the means between two groups. Differences between groups were taken as statistically significant at P < 0.05.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Combination of Gut Microbial Features and the Proteomic Pattern Revealed Changes in Specific Intestinal Luminal Factors and Mechanisms of Their Regulation of Gluten Allergy

Xie

Liu

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Recent research consensus has highlighted the role of intestinal luminal factors in the association between intestinal microenvironment homeostasis and food allergy. However, the association between intestinal immune homeostasis and food allergy-related proteomic features remains elusive. In this study, we aimed to investigate the changes in gluten allergy (GA)-defined phenotypes and endotypes and intestinal microenvironment factors in BALB/c mice and linked GA to colonic proteomic signatures. Combined with increased allergy and diarrhea scores, intense antibody responses and abnormalities in T-cell cytokine production were induced in mice. GA-associated disruption of intestinal microenvironment homeostasis was underlined by the increased colonic pH, decreased intestinal antioxidant capacity, impaired intestinal barrier function, and decreased production and imbalanced proportions of short-chain fatty acids. 16S rRNA amplicon sequencing showed that the gut microbiota dysbiosis in mice was characterized by significant enrichment of six bacterial taxonomic units, including Prevotellaceae, Escherichia Shigella, Alloprevotella, Escherichia coli, Bacteroides vulgatus, and Lachnospiraceae bacterium DW59, which was correlated with immune end points. Using a label-free proteomics quantitative approach, 24 differentially expressed proteins linking GA-induced gut dysbiosis were identified, with four of them enriched in the serine endopeptidase inhibitor activity pathway. The development of GA in mice was associated with changes in specific intestinal luminal factors and may be mediated by serine protease activity-associated metabolic routes.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%