Swelling behaviour and glass transition in genipin-crosslinked chitosan systems

Whitehead, Felicity A.; Young, Simon; Kasapis, Stefan

doi:10.1016/j.ijbiomac.2020.08.178

Cited by 25 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A wide band at 3600–3000 cm −1 is present in the chitosan and hydrogel spectra, which is attributed to overlapping O–H and N–H stretching vibrations. 52 All samples have a band at approximately 2900 cm −1 corresponding to C–H stretching of the pyranose ring, 53,54 in addition to a peak at 2100 cm −1 which originates from C–C of the diamond used in attenuated total reflectance measurement. 42 Genipin's characteristic transmission peaks are observed at 1679 cm −1 and 1618 cm −1 , which represent CC stretching of the carboxymethyl group and CC aromatic stretching, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…57,63 Transmission bands observed is the chitosan spectrum at 1059 cm −1 and 1023 cm −1 are attributed to aliphatic C–N stretching and C–O stretching vibrations in alcohols. 52,60 In the hydrogel spectrum, the band corresponding to aliphatic C–N stretching increases in intensity, likely due to the newly formed tertiary amine. 50 Butler et al reported that the formation of C–N bonds at the expense of C–O bonds occurs during the formation of the heterocyclic genipin–chitosan compound.…”

Section: Resultsmentioning

confidence: 99%

“…1). 32,52 Overlap between the CQO stretching band in secondary amides with the CQC stretching of the olefin ring in genipin causes the amide I band to become slightly broader in curve. 50,56 Fig.…”

Section: Paper Materials Advancesmentioning

confidence: 99%

See 2 more Smart Citations

In vitro evaluation of the biodegradability of chitosan–genipin hydrogels

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

In vitro evaluation of the biodegradability of chitosan–genipin hydrogels

et al. 2022

View full text Add to dashboard Cite

show abstract

“…FTIR spectroscopy was conducted to determine if heat and NaOH-treatment induce chemical changes to native chitosan ( Figure 4 ). In the FTIR spectra of all chitosan samples, a wide band is observed at 3600–3000 cm −1 , which is due to overlapping O-H and N-H stretching vibrations [ 59 ]. A prominent band at approximately 2900 cm −1 is present in all samples, which is attributed to C-H stretching of the pyranose ring [ 9 , 60 ].…”

Section: Resultsmentioning

confidence: 99%

Effective Endotoxin Removal from Chitosan That Preserves Chemical Structure and Improves Compatibility with Immune Cells

et al. 2023

View full text Add to dashboard Cite

Chitosan is one of the most researched biopolymers for healthcare applications, however, being a naturally derived polymer, it is susceptible to endotoxin contamination, which elicits pro-inflammatory responses, skewing chitosan’s performance and leading to inaccurate conclusions. It is therefore critical that endotoxins are quantified and removed for in vivo use. Here, heat and mild NaOH treatment are investigated as facile endotoxin removal methods from chitosan. Both treatments effectively removed endotoxin to below the FDA limit for medical devices (<0.5 EU/mL). However, in co-culture with peripheral blood mononuclear cells (PBMCs), only NaOH-treated chitosan prevented TNF-α production. While endotoxin removal is the principal task, the preservation of chitosan’s structure is vital for the synthesis and lysozyme degradation of chitosan-based hydrogels. The chemical properties of NaOH-treated chitosan (by FTIR-ATR) were significantly similar to its native composition, whereas the heat-treated chitosan evidenced macroscopic chemical and physical changes associated with the Maillard reaction, deeming this treatment unsuitable for further applications. Degradation studies conducted with lysozyme demonstrated that the degradation rates of native and NaOH-treated chitosan-genipin hydrogels were similar. In vitro co-culture studies showed that NaOH hydrogels did not negatively affect the cell viability of monocyte-derived dendritic cells (moDCs), nor induce phenotypical maturation or pro-inflammatory cytokine release.

show abstract

“…A greater amount of research is available because the synthesis of CMCNa-based hydrogel with citric acid as a crosslinking agent can produce higher swelling properties [ 9 , 55 ], offers stability on different pH swelling media [ 84 ], biodegradability, and ensures much better safety [ 11 ] than other crosslinking agents such as ECH, GA, and genipin. Another reason is that GA is more often used as a crosslinking agent for CS-based hydrogel [ 86 , 87 ] and genipin [ 88 , 89 ].…”

Section: Types Of Crosslinking Agents In the Synthesis Of Cellulose-b...mentioning

confidence: 99%

Hydrogel and Effects of Crosslinking Agent on Cellulose-Based Hydrogels: A Review

Nasution

Harahap

Dalimunthe

et al. 2022

Gels

104

View full text Add to dashboard Cite

Hydrogels are hydrophilic polymer materials that can swell but are insoluble in water. Hydrogels can be synthesized with synthetic or natural polymers, but natural polymers are preferred because they are similar to natural tissues, which can absorb a high water content, are biocompatible, and are biodegradable. The three-dimensional structure of the hydrogel affects its water insolubility and ability to maintain its shape. Cellulose hydrogels are preferred over other polymers because they are highly biocompatible, easily accessible, and affordable. Carboxymethyl cellulose sodium (CMCNa) is an example of a water-soluble cellulose derivative that can be synthesized using natural materials. A crosslinking agent is used to strengthen the properties of the hydrogel. Chemical crosslinking agent is used more often than physical crosslinking agent. In this review, article, different types of crosslinking agents are discussed based on synthetic and natural crosslinking agents. Hydrogels that utilize synthetic crosslinking agent have advantages, such as adjustable mechanical properties and easy control of the chemical composition. However, hydrogels that use natural crosslinking agent have better biocompatibility and less latent toxic effect.

show abstract

Swelling behaviour and glass transition in genipin-crosslinked chitosan systems

Cited by 25 publications

References 32 publications

In vitro evaluation of the biodegradability of chitosan–genipin hydrogels

In vitro evaluation of the biodegradability of chitosan–genipin hydrogels

Effective Endotoxin Removal from Chitosan That Preserves Chemical Structure and Improves Compatibility with Immune Cells

Hydrogel and Effects of Crosslinking Agent on Cellulose-Based Hydrogels: A Review

Contact Info

Product

Resources

About