Swelling, Protein Adsorption, and Biocompatibility In Vitro of Gel Beads Prepared from Pectin of Hogweed Heracleum sosnówskyi Manden in Comparison with Gel Beads from Apple Pectin

Popov, Sergey; Paderin, Nikita M.; Khramova, Daria S.; Kvashninova, Elizaveta; Patova, Olga A.; Vityazev, Fedor V.

doi:10.3390/ijms23063388

Cited by 9 publications

(13 citation statements)

References 70 publications

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“…Protein adsorption and leukocyte adhesion on the surface are one of the first events after biomaterial implantation [ 30 , 31 ]. We have previously shown that pectin hydrogels adsorb serum proteins, and adhere and activate leukocytes [ 24 , 32 , 33 ]. In the present study, serum proteins and peritoneal cells did not interfere with the serosal adhesion, suggesting different binding points.…”

Section: Discussionmentioning

confidence: 99%

Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions

Popov

Paderin

Chistiakova

et al. 2023

IJMS

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The study aims to investigate the adhesion of a hydrogel made of cross-linked low-methyl esterified pectin to rat intestinal serosa ex vivo. The adhesivity of the FeP hydrogel, which was cross-linked by Fe3+ cations, exceeded that of hydrogels cross-linked by Ca2+, Zn2+, and Al3+ cations. The concentration of the cross-linking cation failed to influence the adhesion of the pectin hydrogel to the serosa. The mechanical properties and surface microrelief of the pectin hydrogel were influenced by the type and concentration of the cross-linking cations. Fe3+ cations form a harder and more elastic gel than Ca2+ cations. Scanning electron microscopy analysis revealed the characteristic surface pattern of FeP hydrogel and its denser internal structure compared to Ca2+ cross-linked hydrogel. The effect of the salt composition of the adhesion medium was shown since the FeP hydrogel’s adhesion to the serosa was lower in physiological solutions than in water, and adhesion in Hanks’ solution was higher than in phosphate buffered saline. Serum proteins and peritoneal leukocytes did not interfere with the serosal adhesion of the FeP hydrogel. Pre-incubation in Hanks’ solution for 24 h significantly reduced the adhesion of the FeP hydrogel to the serosa, regardless of the pH of the incubation. Thus, serosal adhesion combined with excellent stability and mechanical properties in physiological environments appeared to be advantages of the FeP hydrogel, demonstrating it to be a promising bioadhesive for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions

Popov

Paderin

Chistiakova

et al. 2023

IJMS

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“…Previously, it was shown that the swelling of pectin gel depends on pH. Calcium–pectin gel beads collapsed in phosphate buffer solutions at pH 3.0 and swelled more at pH 7.4 and 8.0 than at pH 5.0 [ 18 ]. Pectin/acrylamide hydrogels in phosphate buffer solutions swelled more in a solution at pH 7.4 than at pH 1.2 and pH 5.4 [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, beads named CaPG, ZnPG, FePG, and AlPG were formed using different cross-linking cations. The pectin gel beads formed were allowed to stand in the respective salt solutions for 24 h and were then washed on the grid three times with 25% aqueous ethanol solution and dried at 25 °C to a constant weight, as described previously [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…The protocol was approved by the Ethical Committee of the Komi Science Centre of the Russian Academy of Sciences. Complement activation evaluation was performed by measuring the C3a levels using an enzyme-linked immunosorbent assay (ELISA) kit (Human C3a ELISA kit, Hycult Biotech, Uden, The Netherlands) as previously described [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…In general, pectin-containing scaffolds are biocompatible [ 7 , 18 ]. However, the biocompatibility of pectin gels formed by different cross-linking cations has not been compared yet.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Cross-Linking Cations on In Vitro Biocompatibility of Apple Pectin Gel Beads

Popov

Paderin

Chistiakova

et al. 2022

IJMS

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The study aimed to compare the in vitro biocompatibility of pectin gels formed by different cross-linking cations. Hydrogel beads named CaPG, ZnPG, FePG, and AlPG were prepared from 4% solutions of apple pectin using ionotropic gelling with CaCl2, ZnCl2, FeCl3, and AlCl3, respectively. Cations influenced the gel strength of the wet gel beads in the following order (least strong) Ca2+ < Zn2+ < Fe3+~Al3+ (most strong). The swelling degree of the CaPG beads after 24 h of incubation in the RPMI-1640 medium was 104%, whereas the ZnPG, FePG, and AlPG beads swelled by 76, 108, and 134%, respectively. The strength of the pectin gel decreased significantly after incubation in the RPMI-1640 medium for 24 h, regardless of the cross-linking cation, although the FePG beads remained the strongest. All the pectin beads adsorbed serum proteins to a low degree, however the serum protein adsorption by the ZnPG and FePG beads (1.46 ± 0.87 and 1.35 ± 0.19 µg/mm2) was more than the CaPG and AlPG beads (0.31 ± 0.36 and 0.44 ± 0.25 µg/mm2). All the pectin beads reduced the production of TNF-α and IL-10 by hPBMCs in response to LPS stimulation. The IL-1β response of cells to LPS was significantly reduced by the CaPG, ZnPG, and FePG beads, whereas the AlPG beads enhanced it twofold. The CaPG, FePG, and AlPG beads had no cytotoxicity. The viability of hPBMCs and human fibroblasts incubated with ZnPG beads was 5.3 and 7.2%, respectively. Thus, the use of different cross-linking cations changed the properties of the pectin gel, which is important for biocompatibility.

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Influence of composition on the internal diffusion mechanism of pectin–starch gel beads

Silveira

Almeida

Andreola

et al. 2022

J of Applied Polymer Sci

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Pectin is a hydrocolloid commonly used as a wall material for encapsulation, meantime using a filler such as starch can improve the retention in the matrix and control the release in specific places. Formulations of pectin and starch were evaluated for bead production by ionic gelation technique using dripping and jet cutter methods. In addition, beads were dried in a fluidized bed and rotating drum at 50°C. The mean diameter, mechanical strength, yield, moisture content, the morphology of wet and dry beads, and rehydration behavior were evaluated. Furthermore, the pomegranate extract (PE) encapsulation efficiency and release in water were measured. The beads with 2.85% solids content of which 70% are pectin and 30%, waxy corn starch proved to be technologically viable and were satisfactorily dried in a fluidized bed, while on a rotating drum, drying was not efficient. Compared with pure pectin, the pectin–starch beads showed a spherical shape, smoother and more homogeneous with greater mechanical strength and greater water absorption capacity after drying in a fluidized bed. The conventional encapsulation of PE added to biopolymer solutions showed low efficiency (4.7%–43.7%) and the release velocity was higher for drip beads (~70%).

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Swelling, Protein Adsorption, and Biocompatibility In Vitro of Gel Beads Prepared from Pectin of Hogweed Heracleum sosnówskyi Manden in Comparison with Gel Beads from Apple Pectin

Cited by 9 publications

References 70 publications

Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions

Serosal Adhesion Ex Vivo of Hydrogels Prepared from Apple Pectin Cross-Linked with Fe3+ Ions

Effect of Cross-Linking Cations on In Vitro Biocompatibility of Apple Pectin Gel Beads

Influence of composition on the internal diffusion mechanism of pectin–starch gel beads

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