SWI/SNF protein complexes participate in the initiation and elongation stages of Drosophila hsp70 gene transcription

Mazina, M. Yu.; Nikolenko, Yu. V.; Краснов, А. Н.; Vorobyeva, Nadezhda E.

doi:10.1134/s1022795416010105

Cited by 9 publications

(2 citation statements)

References 6 publications

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“…Indirect evidence in the literature suggests that the BAF complex may have a role during Pol2 elongation. SWI/SNF components were reported in transcribing gene bodies of yeast and Drosophila [44][45][46][47][48][49] . Brm immunostaining in Drosophila polytene chromatin localizes to active chromatin and depletion of Brm drastically reduced global transcription 46 .…”

Section: Introductionmentioning

confidence: 99%

Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA Polymerase II productive elongation

Cornejo,

Venegas,

Sono

et al. 2023

Preprint

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Signal-dependent RNA Polymerase II (Pol2) productive elongation is an integral component of gene transcription, including those of immediate early genes (IEGs) induced by neuronal activity. However, it remains unclear how productively elongating Pol2 overcome nucleosomal barriers. Using RNAi, three degraders, and several small molecule inhibitors, we show that the mammalian SWI/SNF complex of neurons (neuronal BAF, or nBAF) is required for activity-induced transcription of neuronal IEGs, including Arc. The nBAF complex facilitates promoter-proximal Pol2 pausing, signal-dependent Pol2 recruitment (loading), and importantly, mediates productive elongation in the gene body via interaction with the elongation complex and elongation-competent Pol2. Mechanistically, Pol2 elongation is mediated by activity-induced nBAF assembly (especially, ARID1A recruitment) and its ATPase activity. Together, our data demonstrate that the nBAF complex regulates several aspects of Pol2 transcription and reveal mechanisms underlying activity-induced Pol2 elongation. These findings may offer insights into human maladies etiologically associated with mutational interdiction of BAF functions.

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Section: Introductionmentioning

confidence: 99%

Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA Polymerase II productive elongation

Cornejo,

Venegas,

Sono

et al. 2023

Preprint

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“…The modification introduced by it is recognized by the bromodomains of chromatin remodeling complexes of the SWI/SNF family, through which they are attracted to SAGA-regulated genes [17]. As a result of the active remodeling of the chromatin structure, local nucleosomes are removed or destabilized, which creates favorable conditions for the binding of RNA polymerase to the various transcription factors necessary for the regulation of transcription [18,19]. In addition, ENY2 was found present in the AMEX complex, which interacts with nuclear pore complexes (NPC) and participates in the export of mRNA from the nucleus [14].…”

Section: Introductionmentioning

confidence: 99%

Zinc Finger Protein CG9890 - New Component of ENY2-Containing Complexes of Drosophila

Fursova¹,

Nikolenko²,

Сошникова³

et al. 2018

Acta Naturae

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In previous studies, we showed that the insulator protein Su(Hw) containing zinc finger domains interacts with the ENY2 protein and recruits the ENY2-containing complexes on Su(Hw)-dependent insulators, participating in the regulation of transcription and in the positioning of replication origins. Here, we found interaction between ENY2 and CG9890 protein, which also contains zinc finger domains. The interaction between ENY2 and CG9890 was confirmed. It was established that CG9890 protein is localized in the nucleus and interacts with the SAGA, ORC, dSWI/SNF, TFIID, and THO protein complexes. KEYWORDS ENY2, CG9890, drosophila, immunoprecipitation, zinc fingers. ABBREVIATIONS ENY2 -enhancer of yellow 2; C2H2 -zinc fingers of C2H2 type; SAGA -histone acetyltransferase complex; SWI/SNF -chromatin remodeler; AMEX -mRNA export complex; ORC -origin recognition complex.

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Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized Template and Transcription Assays

Bunch

2023

Methods in Molecular Biology

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SWI/SNF protein complexes participate in the initiation and elongation stages of Drosophila hsp70 gene transcription

Cited by 9 publications

References 6 publications

Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA Polymerase II productive elongation

Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA Polymerase II productive elongation

Zinc Finger Protein CG9890 - New Component of ENY2-Containing Complexes of Drosophila

Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized Template and Transcription Assays

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