Swimming training induces liver adaptations to oxidative stress and insulin sensitivity in rats submitted to high-fat diet

Zacarias, Aline Cruz; Barbosa, Maria Andrea; Guerra‐Sá, Renata; Castro, Uberdan Guilherme Mendes de; Bezerra, Frank Silva; Lima, Wanderson Geraldo de; Cardoso, Leonardo Máximo; Santos, Robson Augusto Souza; Campagnole‐Santos, Maria José; Alzamora, Andréia Carvalho

doi:10.1080/13510002.2017.1315513

Cited by 14 publications

(15 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After seven weeks of the diets, orally by gavage, the treatment with HP β CD/Ang-(1-7) (40 μ g/kg/day) or HP β CD/empty [HP β CD without the inclusion of Ang-(1-7)] was started for six weeks. At the end of the 13 weeks, the rats were fasted for 12 h and decapitated [6, 11]. Blood samples were collected and centrifuged (8000g, 4°C, 6 min).…”

Section: Methodsmentioning

confidence: 99%

“…Frozen liver and gastrocnemius muscle samples (100 mg) were homogenized in phosphate buffer (pH 7.4) and centrifuged at 12.000xg for 10 min at 4°C. Elisa reader at 570 nm was used to determine SOD activity as previously described [6, 16]. CAT activity was measured by the rate of decrease of the hydrogen peroxide (H 2 O 2 ) at 240 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Imbalances in the actions of RAS components can trigger many pathological processes and disturbances in the metabolic functions of the liver and muscle, such as those observed in the metabolic syndrome (MetS) and diabetes mellitus type 2 (DM2) [2–4]. Previous studies have shown the correlation between increased oxidative stress and IR [5, 6] and hyperactivity of the ACE/Ang II/AT1 axis [7, 8]. Increased oxidative stress impairs glucose uptake in the muscle and in the liver, decreasing insulin secretion of pancreatic β cells in DM2 and MetS states [5, 6, 9].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown the correlation between increased oxidative stress and IR [5, 6] and hyperactivity of the ACE/Ang II/AT1 axis [7, 8]. Increased oxidative stress impairs glucose uptake in the muscle and in the liver, decreasing insulin secretion of pancreatic β cells in DM2 and MetS states [5, 6, 9].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antioxidant Effects of Oral Ang-(1-7) Restore Insulin Pathway and RAS Components Ameliorating Cardiometabolic Disturbances in Rats

Figueiredo

Barbosa

Castro

et al. 2019

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

In prevention studies of metabolic syndrome (MetS), Ang-(1-7) has shown to improve the insulin signaling. We evaluated the HPβCD/Ang-(1-7) treatment on lipid metabolism, renin-angiotensin system (RAS) components, oxidative stress, and insulin pathway in the liver and gastrocnemius muscle and hepatic steatosis in rats with established MetS. After 7 weeks of high-fat (FAT) or control (CT) diets, rats were treated with cyclodextrin (HPβCD) or HPβCD/Ang-(1-7) in the last 6 weeks. FAT-HPβCD/empty rats showed increased adiposity index and body mass, gene expression of ACE/ANG II/AT1R axis, and oxidative stress. These results were accompanied by imbalances in the insulin pathway, worsening of liver function, hyperglycemia, and dyslipidemia. Oral HPβCD/Ang-(1-7) treatment decreased ACE and AT1R, increased ACE2 gene expression in the liver, and restored thiobarbituric acid reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), insulin receptor substrate (Irs-1), glucose transporter type 4 (GLUT4), and serine/threonine kinase 2 (AKT-2) gene expression in the liver and gastrocnemius muscle improving hepatic function, cholesterol levels, and hyperglycemia in MetS rats. Overall, HPβCD/Ang-(1-7) treatment restored the RAS components, oxidative stress, and insulin signaling in the liver and gastrocnemius muscle contributing to the establishment of blood glucose and lipid homeostasis in MetS rats.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antioxidant Effects of Oral Ang-(1-7) Restore Insulin Pathway and RAS Components Ameliorating Cardiometabolic Disturbances in Rats

Figueiredo

Barbosa

Castro

et al. 2019

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

show abstract

“…Animals in exercised groups were submitted to a swimming protocol for six weeks, five sessions per week, with an increase in swimming time each week until reaching 55 minutes of swimming (Fig 1). Swimming exercise protocol was adapted from Zacarias et al (2017). The animals were not familiar with swimming, because of this the protocol started with 30 min of swimming and reached 55 min.…”

Section: Experimental Groups and Exercise Trainingmentioning

confidence: 99%

Effects of Type 2 Diabetes Mellitus Induction and of Moderate Physical Exercise on the Hepatic Metabolism and Oxidative Stress in Wistar Rats

Oliveira¹,

Santos²,

Prado³

et al. 2020

Rev. Cont. Saúde

View full text Add to dashboard Cite

AIMS: The aim of the study was to verify if the induction of Type 2 Diabetes Mellitus (T2DM) by the association of streptozotocin and high fat diet causes hepatic alterations and if physical exercise can improve these alterations. METHODS: Forty-six Wistar rats were divided into two groups. One group received a diet containing 60% of fat for 9 weeks and on the 14th day they were injected with a single dose of streptozotocin (25mg/kg). The other group, named control group, received a standard diet and a single dose of citrate buffer was given. Animals were them divided into two subgroups: one sedentary (S) and the other submitted to physical exercise (PE), totalizing four groups: C-S; C-PE; HF-S; HF-PE. After euthanasia blood and liver were collected. Plasma and hepatic concentrations of triacylglycerol and very low density lipoprotein; enzyme alanine transaminase, aspartate transaminase and arginase; urea, lactate and 8-isoprostane (an oxidative stress marker) were dosed. RESULTS: Capillary glucose was significantly higher in HF-S and HF-PE group animals compared to C-S and C-PE groups. There were no significant differences in alanine transaminase, arginase, oxidative stress and lactate concentration among the groups. Plasma urea concentration and hepatic aspartate transaminase concentration was lower in HF-S and HF-PE than in C-PE. Concerning liver triacylglycerol and very low density lipoproteins, both were higher in the HF-PE group when compared to the C-S. CONCLUSIONS: We conclude that diet was decisive for the aforementioned parameters alterations and that physical exercise protocol was not efficient to improve analyzed parameters.

show abstract

Physical training improves thermogenesis and insulin pathway, and induces remodeling in white and brown adipose tissues

et al. 2018

View full text Add to dashboard Cite

Physical training (PT) has been considered as a treatment in metabolic syndrome (MS), since it induces thermogenic activity in brown (BAT) and white (WAT) adipose tissues. We evaluated the therapeutic effect of PT on activity of WAT and BAT in rats with MS induced by high-fat diet (30% lard) for 13 weeks and submitted, for the last 6 weeks, to swimming or kept sedentary (SED) rats. MS-SED rats compared to control diet (CT-SED) rats showed low physical fitness and high levels of glucose, insulin, homeostasis evaluation of insulin resistance (HOMA-IR), homeostasis evaluation of the functional capacity of β-cells (HOMA-β), and blood pressure. The gastrocnemius muscle decreased in peroxisome proliferator-activated receptor gamma coactivator 1-alpha and beta (PGC-1α, PGC-1β), and uncoupled protein 2 and 3 (UCP2 and UCP3) expressions. Both WAT and BAT increased in the adipocyte area and decreased in blood vessels and fibroblast numbers. WAT increased in expression of pro-inflammatory adipokines and decreased in anti-inflammatory adipokine and adiponectin. WAT and gastrocnemius showed impairment in the insulin signaling pathway. In response to PT, MS rats showed increased physical fitness and restoration of certain biometric and biochemical parameters and blood pressure. PT also induced thermogenic modulations in skeletal muscle, WAT and BAT, and also improved the insulin signaling pathway. Collectively, PT was effective in treating MS by inducing improvement in physical fitness and interchangeable effects between skeletal muscle, WAT and BAT, suggesting a development of brown-like adipocyte cells.

show abstract

Swimming training induces liver adaptations to oxidative stress and insulin sensitivity in rats submitted to high-fat diet

Cited by 14 publications

References 47 publications

Antioxidant Effects of Oral Ang-(1-7) Restore Insulin Pathway and RAS Components Ameliorating Cardiometabolic Disturbances in Rats

Antioxidant Effects of Oral Ang-(1-7) Restore Insulin Pathway and RAS Components Ameliorating Cardiometabolic Disturbances in Rats

Effects of Type 2 Diabetes Mellitus Induction and of Moderate Physical Exercise on the Hepatic Metabolism and Oxidative Stress in Wistar Rats

Physical training improves thermogenesis and insulin pathway, and induces remodeling in white and brown adipose tissues

Contact Info

Product

Resources

About