Switchable Enzyme-mimicking catalysts Self-Assembled from de novo designed peptides and DNA G-quadruplex/hemin complex

Supramolecular enzyme-mimicking catalysts self-assembled from peptides

“…Besides the peptide-based cofactor-free peroxidase mimics, we have also designed other peroxidase-mimicking artificial enzymes through the co-assembly of peptides, DNA, and hemin. 73 , 74 , 75 , 76 , 77 In these supramolecular systems, we demonstrated remarkable synergistic effects of peptide and DNA in the enhancement of the catalytic activities.…”

Heme-Dependent Supramolecular Nanocatalysts: A Review

“…This phenomenon is also observed in heme-containing enzyme mimic. Wang and co-workers found that the peptide containing alternative Lys and His residues resulted in the enhancement of the catalytic activity of G-DNA/hemin and accelerated transformation of the G-quadruplex between folding and unfolding states that was triggered through strand displacement mechanism (Figure B). Specifically, they designed a hairpin DNA strand (1) in which the G-quadruplex DNA/hemin DNAzyme sequence was blocked in the stem structure.…”

“…(ii) Schematic operation of the DNA (1)/peptide/hemin complex, triggered by cyclic addition of DNA (2) and (3). Reproduced with permission from ref . Copyright 2022 Elsevier.…”

Oxidase-Mimetic Nanocatalyst Based on Geometry-Dependent Biomolecular Self-Assembly

“…Inspired by the contribution of multicomponent assembly to the bioactive systems' scaffold, researchers have assembled Gquadruplex DNA and β-sheet-forming peptides 22 or chitosan 23 with the hemin cofactor to produce supramolecular peroxidase-mimicking catalysts. Recent studies have shown that the self-assembly of nucleotides with amino acid−based amphiphile aggregates can create a catechol oxidase-like coordination sphere for copper ions.…”

“…These channels can form through protein subunits’ self-assembly in an enzyme complex, such as tryptophan synthase (Figure A, ii). , In these systems, the building block components have complementary shapes that lead to specific interactions and efficient cooperation. Inspired by the contribution of multicomponent assembly to the bioactive systems’ scaffold, researchers have assembled G-quadruplex DNA and β-sheet-forming peptides or chitosan with the hemin cofactor to produce supramolecular peroxidase-mimicking catalysts. Recent studies have shown that the self-assembly of nucleotides with amino acid–based amphiphile aggregates can create a catechol oxidase-like coordination sphere for copper ions .…”