Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Muñoz‐Adalia, Emigdio Jordán; Sanz-Ros, Antonio V.; Flores-Pacheco, Juan Asdrúbal; Hantula, Jarkko; Diez, Julio J.; Vainio, Eeva J.; Fernández, Mercedes

doi:10.3390/f8040127

Cited by 30 publications

(23 citation statements)

References 77 publications

(96 reference statements)

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“…Different bark beetles were shown to be vectors for S. polyspora, which can rapidly spread and colonise trees following beetle attacks [51,52]. In addition, S. polyspora might also benefit from the forest damage caused by other pathogens [53]. As in the present study, the collected needles were healthy-looking, the establishment of S. polyspora was probably latent.…”

Section: Discussionsupporting

confidence: 57%

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

et al. 2020

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The aim was to assess fungal communities associated with living needles and soil of Pinus sylvestris in managed and unmanaged forest stands to get a better understanding of whether and how different intensities of forest management affects fungal diversity and community composition under the north temperate forest zone conditions. The study was carried out in three national parks in Lithuania. Each included five study sites in managed stands and five in unmanaged stands. At each site, three random soil cores and five random last-year needle samples were collected. Following DNA isolation, a DNA fragment of the ITS2 rRNA gene region of each sample was individually amplified and subjected to high-throughput sequencing. Analysis of 195,808 high-quality reads showed the presence of 1909 fungal taxa. Richness and composition of fungal taxa were similar in each substrate (needles and soil) in managed vs. unmanaged sites. The most common fungi in needles were Coleosporium campanulae (12.4% of all fungal sequences), Unidentified sp. 3980_1 (12.4%), Unidentified sp. 3980_4 (4.1%) and Sydowia polyspora (3.1%). In soil: Unidentified sp. 3980_21 (8.6%), Umbelopsis nana (8.2%), Archaeorhizomyces sp. 3980_5 (8.1%) and Penicillium spinulosum (6.3%). The results demonstrated that managed and unmanaged P. sylvestris stands support similar diversity and composition of fungal communities associated with living needles and soil.

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Section: Discussionsupporting

confidence: 57%

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

et al. 2020

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“…This insect species has been proposed as a plausible vector of the disease in the study region, where 12% of the shoots collected from the ground were found to be associated with the pathogen in plantations affected by PPC [22]. However, the pathogen was not found in association with specimens of T. piniperda and T. destruens analyzed by Muñoz-Adalia et al [60] in the same study area, although other Fusarium spp. were detected.…”

Section: Discussionmentioning

confidence: 65%

Fungal Communities Associated with Bark Beetles in Pinus radiata Plantations in Northern Spain Affected by Pine Pitch Canker, with Special Focus on Fusarium Species

Bezos

Martínez‐Álvarez

Sanz-Ros

et al. 2018

Forests

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Fusarium spp., as well as other endophytic or pathogenic fungi that form communities, have been reported to be phoretically associated with bark beetles (Coleoptera; Scolytinae) worldwide. This applies to Fusarium circinatum Nirenberg and O'Donnell, the causal agent of pine pitch canker (PPC), which threatens Pinus radiata D. Don plantations in northern Spain. The main objective of this study was to study the fungal communities associated with bark beetles and their galleries in stands affected by PPC, with special attention given to Fusarium species. Funnel traps and logs were placed in a P. radiata plot known to be affected by F. circinatum. The traps were baited with different attractants: four with (E)-pityol and six with ethanol and α-Pinene. In addition, fresh green shoots with Tomicus piniperda L. feeding galleries were collected from the ground in 25 P. radiata plots affected by PPC. Extracts of whole insects and gallery tissues were plated on agar medium to isolate and identify the associated fungi. A total of 24 different fungal species were isolated from the bark beetle galleries constructed in logs and shoots, while 18 were isolated from the insect exoskeletons. Ten different Fusarium species were isolated from tissue and insects. Fusarium circinatum was isolated from bark beetle exoskeletons (1.05% of the Pityophthorus pubescens Marsham specimens harboured F. circinatum) and from the galleries (3.5% of the T. piniperda feeding galleries harboured the pathogen). The findings provide information about the fungal communities associated with bark beetles in P. radiata stands in northern Spain.

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“…Sydowia polyspora was also found to be the dominant fungal species carried by insects ( Tomicus spp.) feeding on pine shoots, although the fungus–insect–plant interaction remains unclear (Muñoz‐Adalia et al, ).…”

Section: Resultsmentioning

confidence: 99%

First report of Sydowia polyspora causing disease on Pinus pinea shoots

et al. 2019

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The fungus Sydowia polyspora is frequently isolated from conifers worldwide and is considered a pathogen on several hosts. Stone pine (Pinus pinea) is one of the most important forestry species throughout the Mediterranean basin due to the value of the edible pine nut. Stone pines showing tip dieback, needles with tan‐ to yellow‐coloured lesions and shoot death, observed in stands in Portugal, were sampled for analysis. Fungal colonies covered with cream‐coloured spore masses, were consistently obtained. Morphological and phylogenetic analyses of the ITS rDNA region enabled identification of these isolates as S. polyspora. Inoculation tests showed that the fungus caused lesions on excised P. pinea shoots. The symptoms observed might have a negative effect on pine nut production, and thus, evaluation of the impact of this disease is of relevance to future research. This paper is the first to report S. polyspora causing disease on P. pinea.

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Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Cited by 30 publications

References 77 publications

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

Managed and Unmanaged Pinus sylvestris Forest Stands Harbour Similar Diversity and Composition of the Phyllosphere and Soil Fungi

Fungal Communities Associated with Bark Beetles in Pinus radiata Plantations in Northern Spain Affected by Pine Pitch Canker, with Special Focus on Fusarium Species

First report of Sydowia polyspora causing disease on Pinus pinea shoots

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