Symbiotic fungi undergo a taxonomic and functional bottleneck during orchid seeds germination: a case study on Dendrobium moniliforme

Meng, Yuanyuan; Fan, Xu-Li; Zhou, Lin; Shao, Shi-Cheng; Liu, Qiang; Selosse, Marc‐André; Gao, Jiang-Yun

doi:10.1007/s13199-019-00647-x

Cited by 30 publications

(22 citation statements)

References 28 publications

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“…Recent studies have indicated that mycobiont change occurs in most mycorrhizal interaction types, including ericoid (Matsuda et al ., 2012), arbuscular (Mandyam & Jumpponen, 2008), ectomycorrhiza (Clemmensen et al ., 2015), and orchid mycorrhiza (Ercole et al ., 2015). In addition, turnover of mycobionts has been documented at a variety of temporal scales, such as between ontogenetic stages (Bidartondo & Read, 2008; Těšitelová et al ., 2012; Waud et al ., 2017; Meng et al ., 2019; Teste et al ., 2020), seasons (Escudero & Mendoza, 2005; Ercole et al ., 2015; Oja et al ., 2015) and years (McCormick et al ., 2006). However, we have a limited idea of: (1) how frequent or strong such turnover can be; and (2) whether it fits the theoretical background developed above.…”

Section: Introductionmentioning

confidence: 99%

Temporal turnover in mycorrhizal interactions: a proof of concept with orchids

et al. 2021

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Temporal turnover events in biotic interactions involving plants are rarely assessed, although such changes might afford a considerable acclimation potential to the plant. This could enable fairly rapid responses to short-term fluctuations in growth conditions as well as lasting responses to long-term climatic trends. Here, we present a classification of temporal turnover encompassing 11 possible scenarios. Using orchid mycorrhiza as a study model, we show that temporal changes are common, and discuss under which conditions temporal turnover of fungal symbiont is expected. We provide six research questions and identify technical challenges that we deem most important for future studies. Finally, we discuss how the same framework can be applied to other types of biotic interactions.

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Section: Introductionmentioning

confidence: 99%

Temporal turnover in mycorrhizal interactions: a proof of concept with orchids

et al. 2021

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“…The genus Tulasnella is often observed as orchid mycorrhizal fungi in temperate and tropical regions 12,18,19 , and several isolates have been reported to increase seed germination and seedling growth [20][21][22][23][24][25] . Identification of mycorrhizal fungi in South American orchids, mostly conducted in Brazil, has often revealed Tulasnella symbionts: Tulasnella species were isolated from Epidendrum secundum 26,27 , Epidendrum dendrobioides and Sophronits milleri 28 , Oeceoclades maculata, Epidendrum rigidum and Polystachya concreta 29 , E. rigidum and P. concreta 30 .…”

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confidence: 99%

Diversity of mycorrhizal Tulasnella associated with epiphytic and rupicolous orchids from the Brazilian Atlantic Forest, including four new species

Freitas

Silva

et al. 2020

Sci Rep

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the genus Tulasnella often forms mycorrhizas with orchids and has worldwide distribution. Species of this genus are associated with a wide range of orchids, including endangered hosts. initially, species identification relied mostly on morphological features and few cultures were preserved for later phylogenetic comparisons. In this study, a total of 50 Tulasnella isolates were collected from their natural sites in Minas Gerais, Brazil, cultured, and subjected to a phylogenetic analysis based on alignments of sequences of the internal transcribed spacer (itS) of the nuclear ribosomal DnA. our results, based on phylogeny, integrated with nucleotide divergence and morphology, revealed the diversity of isolated Tulasnella species, which included four new species, namely, Tulasnella brigadeiroensis, Tulasnella hadrolaeliae, Tulasnella orchidis and Tulasnella zygopetali. the conservation of these species is important due to their association with endangered orchid hosts and endemic features in the Brazilian Atlantic forest.

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“…The in situ/ex situ seed baiting technique has been suggested as an effective and easy way to obtain seed germination-enhancing fungi [31,32]. Using this method, we successfully obtained e cient germination-enhancing fungi for different Dendrobium species [9][10][11][12], as well as other terrestrial orchids [33,34]. This led to the development of the idea of using seedling-trap experiments to capture seedling growth-promoting fungi in the current study.…”

Section: Discussionmentioning

confidence: 88%

“…In the context of restoration-friendly cultivation, future scienti c research on Dendrobium species, including mycorrhizal technology and microbial fertilizers, is urgently needed [6]. In our previous studies focused on the conservation of over-collected Dendrobium species, we obtained germination-enhancing fungi for different Dendrobium species through an in situ/ex situ seed baiting technique [9][10][11][12], and the fungi have also been successfully used in restoration plantings of medicinal Dendrobium species [13]. However, the dynamics of fungal associations at the seedling stage remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

In Situ Seedling Baiting to Isolate Plant Growth-Promoting Fungi From Dendrobium Officinale, An Over-Collected Medicinal Orchid In China

Chen

Wang

et al. 2021

Preprint

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Background: Orchids are highly dependent on mycorrhizae for survival and growth. Traditionally, to obtain orchid mycorrhizal fungi (OMFs) for plant growth, fungi have been isolated from the roots of mature wild plants; however, the species of OMF may change as the plants undergo different developmental stages. In this study, we developed the idea of using in situ seedling baiting to capture seedling growth-promoting fungi from Dendrobium officinale, an overcollected medicinal orchid in China.Results: In vitro-produced seedlings of D. officinale were transplanted into their original habitats, and newly established roots of well-growing seedlings were sampled for fungal isolation. Roots were sampled at 6 different times over one year, and five Tulasnella species and one Fusarium species were obtained and identified morphologically and molecularly. The ability to promote seedling growth was tested in three Tulasnella species TPYD-1, TPYD-2 and TPYD-3, with high isolation frequencies by inoculation onto in vitro-produced D. officinale seedlings. Although the three fungi were closely related species and clustered together in the phylogenetic tree, they showed different efficiencies in promoting D. officinale seedling growth. Tulasnella TPYD-2 showed a strong ability to promote seedling growth and could be selected for use in restoration plantings of D. officinale.Conclusions: Our results suggest that using an in situ seedling baiting technique could be an efficient way to obtain seedling growth-promoting fungi, and this approach could have broad applications in orchid mycorrhiza studies and orchid conservation.

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Symbiotic fungi undergo a taxonomic and functional bottleneck during orchid seeds germination: a case study on Dendrobium moniliforme

Cited by 30 publications

References 28 publications

Temporal turnover in mycorrhizal interactions: a proof of concept with orchids

Temporal turnover in mycorrhizal interactions: a proof of concept with orchids

Diversity of mycorrhizal Tulasnella associated with epiphytic and rupicolous orchids from the Brazilian Atlantic Forest, including four new species

In Situ Seedling Baiting to Isolate Plant Growth-Promoting Fungi From Dendrobium Officinale, An Over-Collected Medicinal Orchid In China

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