Sympathetic and parasympathetic ganglia express non-NMDA type glutamate receptors: distinct receptor subunit composition in the principle and SIF cells

Kiyama, Hiroshi; Sato, Kohji; Kuba, T; Tohyama, Masaya

doi:10.1016/0169-328x(93)90137-e

Cited by 16 publications

(6 citation statements)

References 16 publications

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“…Such findings, however, do not allow any conclusion with respect to the subunit and splice variant composition. Immunocytochemical studies on the sympathetic superior cervical ganglion revealed immunoreactivity for all AMPA receptor subunits in small intensely fluorescent (SIF) cells and for GluR-A, and -B/C in principle cells [18] being in good agreement with our findings on the neuroblastoma cell line. No information on the AMPA receptor splice preference in the originating neural-crest structure is available to date.…”

Section: Resultssupporting

confidence: 90%

Alternative splicing of AMPA receptor subunits: regulation in clonal cell lines

Christnacher¹,

Sommer²

1995

FEBS Letters

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Section: Resultssupporting

confidence: 90%

Alternative splicing of AMPA receptor subunits: regulation in clonal cell lines

Christnacher¹,

Sommer²

1995

FEBS Letters

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“…Expression of group I metabotropic (see (Shigemoto et al, 1992; Kammermeier and Ikeda 2002; Higashida et al, 2003)), as well as AMPA (Kiyama et al, 1993; Carlton et al, 1998; Coggeshall and Carlton 1999), NMDA (Carlton et al, 1998) (Coggeshall and Carlton 1999) and kainate (Coggeshall and Carlton 1999) ionotropic glutamate receptors in rodent superior cervical ganglion (SCG) (Shigemoto et al, 1992; Kiyama et al, 1993; Kammermeier and Ikeda 2002; Higashida et al, 2003) and LSC (Carlton et al, 1998; Coggeshall and Carlton 1999) neurons and their projections further supports a role of glutamate in the autonomic nervous system. Interestingly, Coggeshall and Carlton (Coggeshall and Carlton 1999) showed that the proportion of NMDA, AMPA and kainate receptors in the gray rami associated with the LSC in rat is considerably increased 48 hr after hindpaw inflammation.…”

Section: Discussionmentioning

confidence: 81%

Some lumbar sympathetic neurons develop a glutamatergic phenotype after peripheral axotomy with a note on VGLUT2-positive perineuronal baskets

Brumovsky

Seroogy

Lundgren

et al. 2011

Experimental Neurology

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Glutamate is the main excitatory neurotransmitter in the nervous system, including in primary afferent neurons. However, to date a glutamatergic phenotype of autonomic neurons has not been described. Therefore, we explored the expression of vesicular glutamate transporters (VGLUTs) type 1, 2 and 3 in lumbar sympathetic chain (LSC) and major pelvic ganglion (MPG) of naïve BALB/C mice, as well as after pelvic nerve axotomy (PNA), using immunohistochemistry and in situ hybridization. Colocalization with activating transcription factor-3 (ATF-3), tyrosine hydroxylase (TH), vesicular acetylcholine transporter (VAChT) and calcitonin generelated peptide was also examined. Sham-PNA, sciatic nerve axotomy (SNA) or naïve mice were included. In naïve mice, VGLUT2-like immunoreactivity (LI) was only detected in fibers and varicosities in LSC and MPG; no ATF-3-immunoreactive (IR) neurons were visible. In contrast, PNA induced upregulation of VGLUT2 protein and transcript, as well as of ATF-3-LI in subpopulations of LSC neurons. Interestingly, VGLUT2-IR LSC neurons coexpressed ATF-3, and often lacked the noradrenergic marker TH. SNA only increased VGLUT2 protein and transcript in scattered LSC neurons. Neither PNA nor SNA upregulated VGLUT2 in MPG neurons. We also found perineuronal baskets immunoreactive either for VGLUT2 or the acetylcholinergic marker VAChT in non-PNA MPGs, usually around TH-IR neurons. VGLUT1-LI was restricted to some varicosities in MPGs, was absent in LSCs, and remained largely unaffected by PNA or SNA. This was confirmed by the lack of expression of VGLUT1 or VGLUT3 mRNAs in LSCs, even after PNA or SNA. Taken together, axotomy of visceral and non-visceral nerves results in a glutamatergic phenotype of some LSC neurons. In addition, we show previously non-described MPG perineuronal glutamatergic baskets.

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“…Glutamate is a major excitatory neurotransmitter in the central nervous system. It has been reported that there is immunoreactivity for AMPA type glutamate receptor in the superior cervical ganglion (SCG) of the rat [13]. Furthermore, glutamate synthesising enzyme glutaminase expression has been reported in the autonomic preganglionic neurons [17].…”

mentioning

confidence: 99%

A part of cholinergic fibers in mouse superior cervical ganglia contain GABA or glutamate

2005

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The localizations and functions of glutamate and GABA, the major amino acid neurotransmitters in the central nervous system, are still unclear in the peripheral nervous system. We immunohistochemically double-stained mouse superior cervical ganglia with antibodies for the vesicular acetylcholine transporter (VAchT), GAD65, the vesicular glutamate transporters 1-3 (VGluTs1-3), the marker of the sympathetic preganglionic neuron (SPN), GABAergic, and glutamatergic terminals, respectively. All GAD65-positive terminals showed VAchT immunoreactivity, indicating that GABAergic fibers originate from SPNs. VGluT2-immunoreactive terminals showing colocalization with VAchT were observed, but VGluT1 and 3 immunoreactive terminals were not. Colocalization of GAD65 and VGluT2 was rarely found. All VGluT2-immunopositive terminals were also immunopositive for neuronal nitric oxide synthase (nNOS), a marker for the subpopulation of the SPNs, while about half of the GABA-immunopositive fibers were immunopositive for nNOS. The origin of these fibers was discussed.

show abstract

Sympathetic and parasympathetic ganglia express non-NMDA type glutamate receptors: distinct receptor subunit composition in the principle and SIF cells

Cited by 16 publications

References 16 publications

Alternative splicing of AMPA receptor subunits: regulation in clonal cell lines

Alternative splicing of AMPA receptor subunits: regulation in clonal cell lines

Some lumbar sympathetic neurons develop a glutamatergic phenotype after peripheral axotomy with a note on VGLUT2-positive perineuronal baskets

A part of cholinergic fibers in mouse superior cervical ganglia contain GABA or glutamate

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