Synapsis with and without recombination in the male meiosis of the leaf-footed bug Holhymenia rubiginosa (Coreidae, Heteroptera)

Toscani, María Ayelén; Pigozzi, María Inés; Bressa, María José; Papeschi, Alba Graciela

doi:10.1007/s10709-007-9159-0

Cited by 7 publications

(12 citation statements)

References 20 publications

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“…In H. rubiginosa heterochromatin variation was not associated with pachytene pairing since meiosis is completely regular (Toscani et al, 2007). The low frequency of cells with a pair of univalents falls within the range observed in other heteropteran insects (Papeschi & Mola, 1990;Mola & Papeschi, 1993;Bressa et al, 1999Bressa et al, , 2001Bressa et al, , 2002.…”

Section: Discussionsupporting

confidence: 63%

“…The m chromosomes are achiasmatic and associate at late prophase I forming a pseudobivalent (pII). During the first meiotic division the m chromosomes and the autosomes divide reductionally but the X chromosome divides equationally (Toscani et al, 2007). During meiosis two bivalents are usually distinguished because of their large size (see below).…”

Section: Karyotype and Male Meiotic Behaviormentioning

confidence: 99%

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Heterochromatin heteromorphism in Holhymenia rubiginosa (Heteroptera: Coreidae)

Bressa¹,

Franco²,

Toscani³

et al. 2008

Eur. J. Entomol.

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Abstract.Heterochromatin is one of the most dynamic components in the genome of species. Previous studies on the heterochromatin content and distribution in Heteroptera (insects with holokinetic chromosomes) have shown that the species belonging to the family Coreidae are interesting model organisms since they show very diverse C bands patterns. In the present work, we analyzed the C-band pattern in individuals of Holhymenia rubiginosa from different populations collected in different years. This species has the diploid karyotype 2n = 27/28 = 24 + 2m + X0/XX (male/female). C-bands are terminally, subterminally or interstitially located on 10-17 chromosomes and a remarkable heterochromatin heteromorphism is observed in the meiotic bivalents: in the presence/absence of bands, in the size of bands and number of bands. A heteromorphism is also inferred in the number of ribosomal genes from the difference in the fluorescent in situ hybridization signals between NOR-homologues. Chiasmata are generally located opposite to conspicuous C-bands, but in some bivalents chiasmata are also observed in close proximity to C-bands. Considering the striking variation in heterochromatin content between individuals and populations it is suggested that heterochromatin should be selectively neutral in H. rubiginosa.

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Section: Discussionsupporting

confidence: 63%

Section: Karyotype and Male Meiotic Behaviormentioning

confidence: 99%

Heterochromatin heteromorphism in Holhymenia rubiginosa (Heteroptera: Coreidae)

Bressa¹,

Franco²,

Toscani³

et al. 2008

Eur. J. Entomol.

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“…In previous reports, the synaptic behaviour of heteropteran sex chromosomes during male meiosis was studied by electron microscopy and silver staining of meiotic axes. The sex chromosomes of several species with different sex chromosome systems (X0, XY, X 1 X 2 0, and X 1 X 2 Y) failed to show the presence of regular axial elements (AEs) during the first meiotic prophase, either in spreads or in sections [Ruthmann and Dahlberg, 1976;Solari, 1979;Suja et al, 2000;Pigozzi and Solari, 2003;Toscani et al, 2008]. These results led to the hypothesis that the lack of formation of AEs along the heteropteran sex chromosomes is somehow related to their equational division at anaphase I (post-reductional behaviour) [Solari, 1979;Suja et al, 2000;Pigozzi and Solari, 2003;Toscani et al, 2008].…”

Section: Meiotic Pairing Of Neo-sex Chromosomes In Dysdercus Albofascmentioning

confidence: 99%

“…It results from a recent finding that the meiotic cohesion protein REC8 localizes along the autosomes in Triatoma species (Reduviidae), but it is absent in the sex chromosomes [Pigozzi and Solari, 2003]. Moreover, the cohesin component SMC3 forms regular AEs along the autosomal bivalents in Graphosoma italicum (Pentatomidae) and Holhymenia rubiginosa (Coreidae), but it is present only as dots or threads in their respective sex chromosomes [Toscani et al, 2008]. Thus, it seems likely that the condensation and cohesion of the sex chromosomes of heteropterans showing post-reductional behaviour is set by rules different from that of autosomes, both in simple and multiple systems [Suja et al, 2000;Pigozzi and Solari, 2003].…”

Section: Meiotic Pairing Of Neo-sex Chromosomes In Dysdercus Albofascmentioning

confidence: 99%

Sex Chromosome Evolution in Cotton Stainers of the Genus <i>Dysdercus</i> (Heteroptera: Pyrrhocoridae)

Bressa

Papeschi

Vítková

et al. 2009

Cytogenet Genome Res

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The neo-X and neo-Y sex chromosomes of Dysdercus albofasciatus represent a unique model for the study of early stages of sex chromosome evolution since they retained the ability to pair and recombine, in contrast to sex chromosomes in most Heteroptera. Here we examined structure, molecular differentiation, and meiotic behaviour of the D. albofasciatus neo-sex chromosomes. Two related species with the ancestral X0 system, D. chaquensis and D. ruficollis, were used for a comparison. In D. albofasciatus, 2 nucleolar organizer regions (NORs) were identified on the neo-X chromosome using fluorescence in situ hybridization (FISH) with an rDNA probe, whereas a single NOR was found on an autosomal pair in the other 2 species. Genomic in situ hybridization (GISH) differentiated a part of the original X in the neo-X chromosome but not the neo-Y chromosome. The same segment of the neo-X chromosome was identified by Zoo-FISH with a chromosome painting probe derived from the X chromosome of D. ruficollis, indicating that this part is conserved between the species. Immunostaining against the cohesin subunit SMC3 revealed that only terminal regions of the D. albofasciatus neo-Xneo-Y bivalent pair and form a synaptonemal complex, which is in keeping with the occurrence of terminal chiasmata, whereas the interstitial region forms a large loop indicating the absence of homology. These results support the hypothesis that the neo-X chromosome evolved by insertion of the original X chromosome into 1 NOR-bearing autosome in an ancestor carrying the X0 system. As a consequence, the homologue of this NOR-autosome became the neo-Y chromosome. A subsequent inversion followed by transposition of the NOR located on the neo-Y onto the neo-X chromosome resulted in the present neo-sex chromosome system in D. albofasciatus.

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“…Finally, the m-chromosomes are generally of the small size, and are usually unpaired and thus achiasmatic during early prophase I. However, previous reports in Coreidae describe the occurrence of regular synapsis of the m-chromosomes ( Toscani et al 2008 ). At late diakinesis they come close each other, and at metaphase I they are always associated end-to-end, i.e.…”

Section: Introductionmentioning

confidence: 96%

Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)

Poggio¹,

Bressa²,

Papeschi³

2011

CCG

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In the present work, we analysed the male meiosis, the content and distribution of heterochromatin and the number and location of nucleolus organizing regions in Microtomus lunifer (Berg, 1900) by means of standard technique, C- and fluorescent bandings, and fluorescent in situ hybridization with an 18S rDNA probe. This species is the second one cytogenetically analysed within the Hammacerinae. Its male diploid chromosome number is 31 (2n=28+X1X2Y), including a minute pair of m-chromosomes. The diploid autosomal number and the presence of m-chromosomes are similar to those reported in Microtomus conspicillaris (Drury, 1782) (2n=28+XY). However, Microtomus lunifer has a multiple sex chromosome system X1X2Y (male) that could have originated by fragmentation of the ancestral X chromosome. Taking into account that Microtomus conspicillaris and Microtomus lunifer are the only two species within Reduviidae that possess m-chromosomes, the presence of this pair could be a synapomorphy for the species of this genus. C- and fluorescent bandings showed that the amount of heterochromatin in Microtomus lunifer was small, and only a small CMA3 bright band was observed in the largest autosomal pair at one terminal region. FISH with the 18S rDNA probe demonstrated that ribosomal genes were terminally placed on the largest autosomal pair. Our present results led us to propose that the location of rDNA genes could be associated with variants of the sex chromosome systems in relation with a kind of the sex chromosome systems within this family. Furthermore, the terminal location of NOR in the largest autosomal pair allowed us to use it as a chromosome marker and, thus, to infer that the kinetic activity of both ends is not a random process, and there is an inversion of this activity.

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Synapsis with and without recombination in the male meiosis of the leaf-footed bug Holhymenia rubiginosa (Coreidae, Heteroptera)

Cited by 7 publications

References 20 publications

Heterochromatin heteromorphism in Holhymenia rubiginosa (Heteroptera: Coreidae)

Heterochromatin heteromorphism in Holhymenia rubiginosa (Heteroptera: Coreidae)

Sex Chromosome Evolution in Cotton Stainers of the Genus <i>Dysdercus</i> (Heteroptera: Pyrrhocoridae)

Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae)

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