Synaptic Computation and Sensory Processing in Neocortical Layer 2/3

Petersen, Carl C. H.; Crochet, Sylvain

doi:10.1016/j.neuron.2013.03.020

Cited by 238 publications

(247 citation statements)

References 184 publications

(309 reference statements)

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“…The average number of neurons that could be detected simultaneously with a single octrode was 1.16 Ϯ 0.4 (range: 0 -8) in the thalamus and 0.6 Ϯ 0.3 (range: 0 -6) in the cortex. In our opinion, the putative reasons behind the lower cortical unit yield are the sparse firing of neurons located in the supragranular cortical layers (de Kock et al 2007;de Kock and Sakmann 2009;Petersen and Crochet 2013;Sakata and Harris 2009) and the smaller dorsoventral extension of the cortex compared with the thalamus, but we cannot exclude the possibility that the im- Fig. 2.…”

Section: Sua Recorded With the Edc Probementioning

confidence: 91%

Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe

Fiáth

Beregszászi

Horváth

et al. 2016

Journal of Neurophysiology

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Fiáth R, Beregszászi P, Horváth D, Wittner L, Aarts AA, Ruther P, Neves HP, Bokor H, Acsády L, Ulbert I. Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe. J Neurophysiol 116: 2312-2330. First published August 17, 2016 doi:10.1152/jn.00318.2016.-Recording simultaneous activity of a large number of neurons in distributed neuronal networks is crucial to understand higher order brain functions. We demonstrate the in vivo performance of a recently developed electrophysiological recording system comprising a two-dimensional, multi-shank, high-density silicon probe with integrated complementary metal-oxide semiconductor electronics. The system implements the concept of electronic depth control (EDC), which enables the electronic selection of a limited number of recording sites on each of the probe shafts. This innovative feature of the system permits simultaneous recording of local field potentials (LFP) and single-and multiple-unit activity (SUA and MUA, respectively) from multiple brain sites with high quality and without the actual physical movement of the probe. To evaluate the in vivo recording capabilities of the EDC probe, we recorded LFP, MUA, and SUA in acute experiments from cortical and thalamic brain areas of anesthetized rats and mice. The advantages of large-scale recording with the EDC probe are illustrated by investigating the spatiotemporal dynamics of pharmacologically induced thalamocortical slow-wave activity in rats and by the two-dimensional tonotopic mapping of the auditory thalamus. In mice, spatial distribution of thalamic responses to optogenetic stimulation of the neocortex was examined. Utilizing the benefits of the EDC system may result in a higher yield of useful data from a single experiment compared with traditional passive multielectrode arrays, and thus in the reduction of animals needed for a research study. electronic depth control; silicon probe; high-density recording; thalamocortical oscillation; optogenetics DESPITE THE RAPID ADVANCEMENT of brain imaging techniques offering both high spatial and temporal resolution, electrophysiological tools are still widely used methods to investigate the complex spatiotemporal activity patterns of neuronal circuits. Over the past few decades, single-wire electrodes used for in vivo extracellular recording of action potentials evolved into multielectrode arrays covering the range of up to 1,000 recording sites NEW & NOTEWORTHY Recording the electrical activity of a large number of neurons located in the thalamocortical

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Section: Sua Recorded With the Edc Probementioning

confidence: 91%

Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe

Fiáth

Beregszászi

Horváth

et al. 2016

Journal of Neurophysiology

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“…Cortical states are typically defined by the degree of shared fluctuations among cortical neural populations, measured by local field potential (LFP) frequency power (Harris and Thiele, 2011), and neural population correlations (Kohn et al, 2009). Cortical states exert profound effects on sensory responses (Haider and McCormick, 2009;Petersen and Crochet, 2013), but there remain unresolved questions about cortical states and their effects on sensory perception.…”

Section: Introductionmentioning

confidence: 99%

Cortical states control visual spatial perception

Speed

Rosario

Burgess

et al. 2018

Preprint

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SummaryMany factors modulate the state of cortical activity, but the importance of cortical states for sensory perception remains debated.We trained mice to detect spatially localized visual stimuli, and simultaneously measured local field potentials and excitatory and inhibitory neuron populations across layers of primary visual cortex (V1). Cortical states with low firing rates and correlations between excitatory neurons, and reduced oscillatory activity in Layer 4, accurately predicted single trials of visual spatial detection behavior. Our results show that cortical states exert strong effects at the initial stage of cortical processing in V1, and play a decisive role for visual spatial behavior in mice.

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“…However, this widely used synthetic indicator has insufficient sensitivity to detect Ca 2+ transients evoked by single action potentials under in vivo conditions [26]. This sensitivity is extremely important in studies of the topographic organization of the Au1, because many cortical neurons respond to sound stimulation with only one or two spikes, particularly in layer 2/3 [27,28]. In addition, Bandyopadhyay et al and Rothschild et al have imaged the mouse Au1 with fluo-4 [9,15], a synthetic dye with a higher detection sensitivity and a better signal-to-noise ratio (SNR) than OGB-1 [9,29].…”

Section: Introductionmentioning

confidence: 99%

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice

Zhang

Wang

et al. 2017

Biomed. Opt. Express

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Abstract:The organization in the primary auditory cortex (Au1) is critical to the basic function of auditory information processing and integration. However, recent mapping experiments using in vivo two-photon imaging with different Ca 2+ indicators have reached controversial conclusions on this topic, possibly because of the different sensitivities and properties of the indicators used. Therefore, it is essential to identify a reliable Ca 2+ indicator for use in in vivo functional imaging of the Au1, to understand its functional organization. Here, we demonstrate that a previously reported indicator, Cal-520, performs well in both anesthetized and awake conditions. Cal-520 shows a sufficient sensitivity for the detection of single action potentials, and a high signal-to-noise ratio. Cal-520 reliably reported on both spontaneous and sound-evoked neuronal activity in anesthetized and awake mice. After testing with pure tones at a range of frequencies, we confirmed the local heterogeneity of the functional organization of the mouse Au1. Therefore, Cal-520 is a reliable and useful Ca 2+ indicator for in vivo functional imaging of the Au1.

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Synaptic Computation and Sensory Processing in Neocortical Layer 2/3

Cited by 238 publications

References 184 publications

Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe

Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe

Cortical states control visual spatial perception

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice

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