Synaptic plasticity and the analysis of the field-EPSP as well as the population spike using separate recording electrodes in the dentate gyrus in freely moving rats

Frey, Sabine; Frey, Julietta U.

doi:10.1016/j.jneumeth.2009.07.024

Cited by 10 publications

(12 citation statements)

References 55 publications

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“…Recording of the fEPSPs alone does not allow characterization of postsynaptic spike generation in response to stimuli. For optimal characterization of stimulus parameters, we must record both fEPSPs and the population spike [52]. …”

Section: Discussionmentioning

confidence: 99%

Postnatal Systemic Inflammation Exacerbates Impairment of Hippocampal Synaptic Plasticity in an Animal Seizure Model

Chen

Kuo

Chu

et al. 2013

Neuroimmunomodulation

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Objective: To investigate the effects of systemic inflammation in the critical postnatal stages on neurophysiological actions of immune processes and neural plasticity in adult rats after kainic acid (KA)-induced seizures. Methods: To determine changes in hippocampal synaptic plasticity after postnatal central nervous system inflammatory responses and seizure attacks, we performed intraperitoneal injections of lipopolysaccharide (LPS) in postnatal Sprague Dawley rats on day 14 (P14) to induce central nervous system inflammation. We then used a KA tail vein injection on P35 to induce seizure attacks. We compared the variability in synaptic plasticity in the hippocampal Schaffer collateral-CA1 region of seizure animals with or without LPS-induced inflammation preconditioning. Results: P14 injection of LPS increased susceptibility to seizures, while treatment with KA on P35 induced seizures. Long-term potentiation (LTP) of the Schaffer collateral-CA1 region was impaired in seizure animals, and this effect was more pronounced in the P14 LPS injection group. Fluoro-Jade staining revealed an increase in degenerated hippocampal CA1 pyramidal cells in the P14 LPS injection group. Cytokine expression in the hippocampus in the pre-, peri- and postictus periods was greater in P14 LPS rats than in saline-treated rats. Conclusions: Intraperitoneal LPS injection on P14 induces higher cytokine secretion after KA-induced seizures, enhancing neuronal excitability, shortening seizure onset time and exacerbating neuronal degeneration and impairment of LTP formation in the hippocampal Schaffer collateral-CA1 region. Central nervous system inflammation during critical stages of childhood development could disrupt the balance needed for neurophysiological actions of immune processes, producing direct, pernicious effects on memory, neural plasticity and neurogenesis into adulthood.

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Section: Discussionmentioning

confidence: 99%

Postnatal Systemic Inflammation Exacerbates Impairment of Hippocampal Synaptic Plasticity in an Animal Seizure Model

Chen

Kuo

Chu

et al. 2013

Neuroimmunomodulation

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“…Recently, a similar approach was introduced by our lab which allows the stable recording of the both parameters in the dentate gyrus in freely behaving animals (Frey and Frey, 2009). This method allowed reliably longterm recordings of the field-EPSP as well the population spike in their places of generation.…”

Section: Discussionmentioning

confidence: 99%

“…Recording of just the field-EPSP does not allow to characterize postsynaptic spike generation per stimulus. For a better characterization of the used stimulus parameters and the inter-comparison (standardization) of used stimulation intensities between different animals, the recording of both, the EPSP as well as the spike is useful (see also Frey and Frey, 2009). As mentioned above, the stimulation strength for induction of different forms of long-lasting changes in synaptic plasticity is not only dependent on the applied pattern, its frequency and number of stimuli but also on the stimulus intensity Navakkode et al, 2007;Reymann and Frey, 2007;Sajikumar et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we established a method for simultaneously recording of both, the field-EPSP as well as the population spike using separate recording electrodes in the dentate gyrus in freely moving rats (Frey and Frey, 2009). This methods allowed stable recordings of both parameters over the entire experimental session and the characterization of both potentials during different forms of synaptic plasticity.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous recording of the field-EPSP as well as the population spike in the CA1 region in freely moving rats by using a fixed “double”-recording electrode

Scherf

Frey

2010

Journal of Neuroscience Methods

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“…Monitoring the evoked field potentials during implantation controlled the correct placement, especially with regard to electrode depth. By placement of the recording electrode into the granular cell layer, we can assume that a measured population spike reflects summed action potentials of granular cells, because the electrode is located close to the side of the action potential generation (Frey and Frey, 2009). Because granular cells are the principal cells in the dentate gyrus, observed variations in the population spike amplitude and latency indicate an altered output activity from the dentate gyrus.…”

Section: Animals and Surgical Proceduresmentioning

confidence: 99%

NMDA-Dependent Mechanisms Only Affect the BOLD Response in the Rat Dentate Gyrus by Modifying Local Signal Processing

Tiede

Krautwald

Fincke

et al. 2011

J Cereb Blood Flow Metab

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The role of N-methyl-D-aspartate (NMDA) receptor-mediated mechanisms in the formation of a blood oxygen level-dependent (BOLD) response was studied using electrical stimulation of the right perforant pathway. Stimulation of this fiber bundle triggered BOLD responses in the right hippocampal formation and in the left entorhinal cortex. The perforant pathway projects to and activates the dentate gyrus monosynaptically, activation in the contralateral entorhinal cortex is multisynaptic and requires forwarding and processing of signals. Application of the NMDA receptor antagonist MK801 during stimulation had no effect on BOLD responses in the right dentate gyrus, but reduced the BOLD responses in the left entorhinal cortex. In contrast, application of MK801 before the first stimulation train reduced the BOLD response in both regions. Electrophysiological recordings revealed that the initial stimulation trains changed the local processing of the incoming signals in the dentate gyrus. This altered electrophysiological response was not further changed by a subsequent application of MK801, which is in agreement with an unchanged BOLD response. When MK801 was present during the first stimulation train, a dissimilar electrophysiological response pattern was observed and corresponds to an altered BOLD response, indicating that NMDA-dependent mechanisms indirectly affect the BOLD response, mainly via modifying local signal processing and subsequent propagation.

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Synaptic plasticity and the analysis of the field-EPSP as well as the population spike using separate recording electrodes in the dentate gyrus in freely moving rats

Cited by 10 publications

References 55 publications

Postnatal Systemic Inflammation Exacerbates Impairment of Hippocampal Synaptic Plasticity in an Animal Seizure Model

Postnatal Systemic Inflammation Exacerbates Impairment of Hippocampal Synaptic Plasticity in an Animal Seizure Model

Simultaneous recording of the field-EPSP as well as the population spike in the CA1 region in freely moving rats by using a fixed “double”-recording electrode

NMDA-Dependent Mechanisms Only Affect the BOLD Response in the Rat Dentate Gyrus by Modifying Local Signal Processing

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